Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
The restaurant at the end of the random walk: recent developments in the description of anomalous transport by fractional dynamics
Ralf Metzler,Joseph Klafter +1 more
TL;DR: Fractional dynamics has experienced a firm upswing during the past few years, having been forged into a mature framework in the theory of stochastic processes as mentioned in this paper, and a large number of research papers developing fractional dynamics further, or applying it to various systems have appeared since our first review article on the fractional Fokker-Planck equation.
Journal ArticleDOI
Dynamic personalities of proteins.
TL;DR: The dream is to 'watch' proteins in action in real time at atomic resolution, which requires addition of a fourth dimension, time, to structural biology so that the positions in space and time of all atoms in a protein can be described in detail.
Journal ArticleDOI
Fluorescence Lifetime Measurements and Biological Imaging
TL;DR: The lifetime of a photophysical process is the time required by a population of N electronically excited molecules to be reduced by a factor of e via the loss of energy through fluorescence and other non-radiative processes and the average length of time τ is called the mean lifetime, or simply lifetime.
Journal ArticleDOI
Anomalous diffusion models and their properties: non-stationarity, non-ergodicity, and ageing at the centenary of single particle tracking.
TL;DR: This Perspective is intended as a guidebook for both experimentalists and theorists working on systems, which exhibit anomalous diffusion, and pays special attention to the ergodicity breaking parameters for the different anomalous stochastic processes.
References
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Journal ArticleDOI
Electron Transfer In Proteins
TL;DR: Analyses of electronic-coupling strengths suggest that the efficiency of long-range ET depends on the protein secondary structure: beta sheets appear to mediate coupling more efficiently than alpha-helical structures, and hydrogen bonds play a critical role in both.
Book ChapterDOI
Electron transfer : from isolated molecules to biomolecules
Mordechai Bixon,Joshua Jortner +1 more
TL;DR: In this article, the authors proposed a model for medium reorganization and donor-acceptor coupling for long-range and multicenter Electron Transfer in the gas phase.
Journal ArticleDOI
A single-molecule study of RNA catalysis and folding.
Xiaowei Zhuang,Laura E. Bartley,Hazen P. Babcock,Rick Russell,Taekjip Ha,Daniel Herschlag,Steven Chu +6 more
TL;DR: Results establish single-molecule fluorescence as a powerful tool for examining RNA folding by observing previously established folding pathways and a pathway with an observed folding rate constant of 1 per second was discovered.
Journal ArticleDOI
Anomalous Diffusion and Relaxation Close to Thermal Equilibrium: A Fractional Fokker-Planck Equation Approach
TL;DR: In this paper, a fractional Fokker-Planck equation describing the stochastic evolution of a particle under the combined influence of an external, nonlinear force and a thermal heat bath is introduced.
Journal ArticleDOI
Optical studies of single molecules at room temperature
X. Sunney Xie,Jay K. Trautman +1 more
TL;DR: Recent developments in optical studies of single molecules at room temperature are reviewed, with an emphasis on the underlying principles and the potential of single-molecule experiments.