Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Proceedings ArticleDOI
Avalanche buildup and propagation effects on photon-timing jitter in Si-SPAD with non-uniform electric field
Antonino Ingargiola,Mattia Assanelli,Andrea Gallivanoni,Ivan Rech,Massimo Ghioni,Sergio Cova +5 more
TL;DR: In this article, the contribution of the avalanche buildup statistics and the lateral avalanche propagation to the photon-timing jitter in silicon SPAD devices was investigated, in which both local and non-local models for impact ionization were considered.
Journal ArticleDOI
Proteins fold by subdiffusion of the order parameter.
Amandeep K. Sangha,Tom Keyes +1 more
TL;DR: The fractal SE theory is completely characterized by short-time simulations, and its predictions are in quantitative agreement with simulated long-time folding dynamics, and may serve as an accelerated algorithm to study the folding of proteins too slow to be simulated directly.
Journal ArticleDOI
Relay stations for electron hole migration in peptides: possibility for formation of three-electron bonds along peptide chains.
TL;DR: It is inferred that a series of three-electron bonds may be formed during the electron hole migration along the peptide backbone in proteins and assist electron hole transport as relay stations, supporting the peptides chain as a conduction wire.
Journal ArticleDOI
Counting statistics of single molecule reaction events and reaction dynamics of a single molecule
TL;DR: In this paper, an exact form for counting statistics of single-molecule (SM) reaction events for a molecule with arbitrary hidden dynamical processes coupled to the reaction, examples of which can be found in many single biopolymer experiments.
Journal ArticleDOI
Aromatic Residues Regulating Electron Relay Ability of S-Containing Amino Acids by Formations of S∴π Multicenter Three-Electron Bonds in Proteins
TL;DR: In this paper, the side chains of four aromatic amino acids (Phe, His, Tyr, and Trp residues) may promote methionine and cystine residues to participate in the protein electron hole transport by the formation of special multicenter, three-electron bonds (S∴π) between the S-atoms and the aromatic rings.
References
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Journal ArticleDOI
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Journal ArticleDOI
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Journal ArticleDOI
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