Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Kramers-like escape driven by fractional Gaussian noise.
TL;DR: This work investigates the escape from a potential well of a test particle driven by fractional Gaussian noise with Hurst exponent H and finds that the escape becomes increasingly faster for decreasing values of H, consistent with previous findings on the first passage behavior.
Journal ArticleDOI
Allosteric vs. spontaneous exit-site (E-site) tRNA dissociation early in protein synthesis
Chunlai Chen,Benjamin Stevens,Jaskiran Kaur,Zeev Smilansky,Barry S. Cooperman,Yale E. Goldman +5 more
TL;DR: The results suggest that the distribution of pretranslocation tRNA states and posttranslocation pathways are correlated within each elongation cycle via communication between distant subdomains in the ribosome, but that this correlation between lengthening cycle intermediates does not persist into succeeding cycles.
Journal ArticleDOI
Proteins in action: femtosecond to millisecond structural dynamics of a photoactive flavoprotein.
Richard Brust,Andras Lukacs,Andras Lukacs,Allison Haigney,Kiri Addison,Agnieszka A. Gil,Michael Towrie,Ian P. Clark,Gregory M. Greetham,Peter J. Tonge,Stephen R. Meech +10 more
TL;DR: A point mutation which suppresses biological function is shown to ‘short circuit’ this structural relaxation pathway, suppressing the changes which occur further away from the chromophore while accelerating dynamics close to it.
Journal ArticleDOI
Khinchin Theorem and Anomalous Diffusion
Luciano C. Lapas,Luciano C. Lapas,Rafael Morgado,Mendeli H. Vainstein,Mendeli H. Vainstein,J. Miguel Rubi,Fernando A. Oliveira +6 more
TL;DR: It is shown that for all ranges of normal and anomalous diffusion described by a generalized Langevin equation the Khinchin theorem holds.
Journal ArticleDOI
Superstatistical generalised Langevin equation: non-Gaussian viscoelastic anomalous diffusion
TL;DR: In this article, a stochastic model based on a generalised Langevin equation was proposed, in which non-Gaussian shapes of the probability density function and normal or anomalous diffusion have a common origin.
References
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Journal ArticleDOI
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Journal ArticleDOI
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