Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Exact analytical evaluation of time dependent transmission coefficient from the method of reactive flux for an inverted parabolic barrier.
TL;DR: An elegant way of combining the normal mode analysis and the method of reactive flux to evaluate the time dependent transmission coefficient for a classical particle coupled to a set of harmonic oscillators, surmounting a one dimensional barrier is demonstrated.
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Describing the dynamics of processes consisting simultaneously of Poissonian and non-Poissonian kinetics
Stephan Eule,Rudolf Friedrich +1 more
TL;DR: In this article, the authors provide a concise treatment of such random systems which are affected by regular and non-Poissonian kinetics at the same time, and provide a controlled approximation scheme for this equation.
Proceedings ArticleDOI
High-performance silicon single-photon avalanche diode array
TL;DR: In this paper, the authors present two possible approaches to make SPAD array more and more competitive: employing a standard industrial CMOS technology or developing a dedicated technology. And they discuss several issues: dark counts, quantum efficiency, crosstalk, timing performance.
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Origin of 1/f noise in hydration dynamics on lipid membrane surfaces
TL;DR: The results suggest that the 1/f noise attributed to the correlated renewal process may contribute to the stability of the hydration layers and lipid membranes.
Journal ArticleDOI
Electron transfer reaction in a single protein molecule observed by total internal reflection fluorescence microscopy.
TL;DR: The protein ET reaction was found to be explained by the distribution of the rate constants, and it is suggested that the origin of this rate distribution is the distance change associated with the structural fluctuation in the protein molecule.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
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Rudolph A. Marcus,Norman Sutin +1 more
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Journal ArticleDOI
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Journal ArticleDOI
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