Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Protein physics: Forever ageing
TL;DR: This article showed that the dynamics inside single proteins is not as simple as we thought, and that proteins are forever changing, and showed that protein dynamics inside a single molecule can change with time.
Journal ArticleDOI
Non-Markovian properties and multiscale hidden Markovian network buried in single molecule time series
Tahmina Sultana,Hiroaki Takagi,Miki Morimatsu,Hiroshi Teramoto,Chun-Biu Li,Yasushi Sako,Tamiki Komatsuzaki +6 more
TL;DR: A novel scheme to extract a multiscale state space network (SSN) from single-molecule time series is presented and an explicit analytical expression of the autocorrelation function in terms of the SSN is derived.
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Factors underlying asymmetric pore dynamics of disaggregase and microtubule-severing AAA+ machines.
TL;DR: In this article, the authors probed the asymmetric pore motions and intra-ring interactions that support them by performing extensive molecular dynamics simulations of single-ring severing proteins and the double-ring disaggregase ClpB.
Journal ArticleDOI
Langevin dynamics of correlated subdiffusion and normal diffusion.
TL;DR: The dissipative dynamics of a particle governed by a two-dimensional generalized Langevin equation with coupled fractional Gaussian noise and white noise in its respective coordinates is analyzed and the effects of fluctuation correlation coupling and interoscillator coupling are elucidated.
Shallow-trench-isolation bounded single-photon avalanche diodes in commercial deep submicron CMOS technologies
TL;DR: In this paper, the first single-photon detection device to be manufactured in a commercial deep- sub-micron CMOS technology was described, and an area-and power-efficient method was presented, analyzed, and experimentally examined.
References
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Journal ArticleDOI
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