Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
DNA-ligand Binding Mode Discrimination by Characterizing Fluorescence Resonance Energy Transfer Through Lifetime Measurements with Picosecond Resolution
TL;DR: A clear signature is found that distinguishes between the two binding mechanisms: minor groove binding induces an asymptotic decrease of the D fluorescence lifetime, while intercalation gives a monotonically increasing lifetime and the appearance of an additional short lifetime.
Journal ArticleDOI
The motions of an enzyme soloist
TL;DR: Yang et al. as mentioned in this paper showed that electron transfer from the fluorophore to a quencher induces fluctuations of the fluorescence lifetime along with the distance to the quenchers.
Posted ContentDOI
Aging power spectrum of membrane protein transport and other subordinated random walks
TL;DR: In this paper, the aging Wiener-Khinchin theorem is used to derive the power spectral density of fractional Brownian motion coexisting with a scale-free continuous time random walk.
Journal ArticleDOI
Molecular Gating of an Engineered Enzyme Captured in Real Time.
Piia Kokkonen,Jan Sykora,Zbynek Prokop,Avisek Ghose,David Bednar,Mariana Amaro,Koen Beerens,Šárka Bidmanová,Michaela Slanska,Jan Brezovsky,Jiri Damborsky,Martin Hof +11 more
TL;DR: The dynamics of a molecular gate artificially introduced into an access tunnel of the most efficient haloalkane dehalogenase is analyzed using pre-steady-state kinetics, single-molecule fluorescence spectroscopy, and molecular dynamics.
Journal ArticleDOI
Confinement and viscoelastic effects on chain closure dynamics
TL;DR: In this article, the scaling relationship between the mean closure time of a flexible chain and the length of its contour was investigated under two conditions: (a) confinement of the chain to a sphere of radius d and (b) modulation of its dynamics by colored Gaussian noise.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
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Rudolph A. Marcus,Norman Sutin +1 more
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Journal ArticleDOI
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Journal ArticleDOI
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TL;DR: The progress in applying single-molecule detection and single-Molecule spectroscopy at room temperature by laser-induced fluorescence with the use of fluorophores that are site-specifically attached to macromolecules is reviewed.