Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Plasmonics for near-field nano-imaging and superlensing
TL;DR: In this article, surface plasmons propagating along the metal surface can help to achieve superlensing, in which perfect imaging is possible through a flat thin metal film, but can also provide nano-imaging of practical samples by using a localized surface plasmon mode at the tip of a metallic nanoprobe.
Journal ArticleDOI
Analysis of Single-Molecule FRET Trajectories Using Hidden Markov Modeling
TL;DR: An analysis scheme is developed that casts single-molecule time-binned FRET trajectories as hidden Markov processes, allowing one to determine, based on probability alone, the most likely FRET-value distributions of states and their interconversion rates while simultaneously determining the mostlikely time sequence of underlying states for each trajectory.
Journal ArticleDOI
Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited
Brian P. English,Wei Min,Antoine M. van Oijen,Kang Taek Lee,Kang Taek Lee,Guobin Luo,Hongye Sun,Hongye Sun,Binny J. Cherayil,Binny J. Cherayil,Samuel C. Kou,X. Sunney Xie +11 more
TL;DR: It is proved that the Michaelis-Menten equation still holds even for a fluctuating single enzyme, but bears a different microscopic interpretation.
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Advances in Single-Molecule Fluorescence Methods for Molecular Biology
TL;DR: In this exciting era of transitioning from in vitro to in vivo and in situ conditions, it is anticipated that SM fluorescence methodology will become a common tool of molecular biology.
Journal ArticleDOI
Single-Molecule Fluorescence Studies of Protein Folding and Conformational Dynamics
TL;DR: A review of single-molecule methods in chemistry to provide a rapid description of the main technical approaches and focus on a few illustrative examples of their elucidative power, and describes applications of a unique set of methods to biochemical questions and, more specifically, the elucidation of protein structure, dynamics, and function.
References
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Electron transfers in chemistry and biology
Rudolph A. Marcus,Norman Sutin +1 more
TL;DR: In this paper, the electron transfer reactions between ions and molecules in solution have been the subject of considerable experimental study during the past three decades, including charge transfer, photoelectric emission spectra, chemiluminescent electron transfer, and electron transfer through frozen media.
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Formation of glasses from liquids and biopolymers.
TL;DR: The onset of a sharp change in ddT( is the Debye-Waller factor and T is temperature) in proteins, which is controversially indentified with the glass transition in liquids, is shown to be general for glass formers and observable in computer simulations of strong and fragile ionic liquids, where it proves to be close to the experimental glass transition temperature.
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The energy landscapes and motions of proteins.
TL;DR: The concepts that emerge from studies of the conformational substates and the motions between them permit a quantitative discussion of one simple reaction, the binding of small ligands such as carbon monoxide to myoglobin.
Journal ArticleDOI
Fluorescence spectroscopy of single biomolecules.
TL;DR: The progress in applying single-molecule detection and single-Molecule spectroscopy at room temperature by laser-induced fluorescence with the use of fluorophores that are site-specifically attached to macromolecules is reviewed.