Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Direct single-molecule counting for immunoassay applications.
TL;DR: The utility of using single-molecule counting on eluted detection conjugate, following the capture and sandwich formation portions of the assay having been completed on microparticles is demonstrated, creating the opportunity for a universal detection platform that can be used to perform a variety of diagnostic and blood screening assays.
DissertationDOI
An Investigation of the Diagnostic Potential of Autofluorescence Lifetime Spectroscopy and Imaging for Label-Free Contrast of Disease
TL;DR: This paper presents a meta-anatomy of the gastrointestinal tract, conventional endoscopy and advanced endoscopic techniques, and some of the techniques used in these techniques, as well as some new techniques and approaches, developed since the publication of this paper in 2016.
Journal ArticleDOI
Simulated data sets for single molecule kinetics: some limitations and complications of data analysis
TL;DR: Parallel experiments with Monte Carlo simulations of potential models hypothesized to describe the reaction kinetics reveal some of the challenges of interpreting finite single-molecule data sets by employing various statistical signatures that have been identified.
Journal ArticleDOI
Monte carlo simulations of protein assembly, disassembly, and linear motion on DNA.
TL;DR: The potential of the Monte Carlo simulations used for the analysis of a number of single-molecule experiments, for example, the binding of the DNA-repair proteins RecA and Rad51 to DNA, are discussed.
Journal ArticleDOI
Singlet oxygen luminescence detection with a fiber-coupled superconducting nanowire single-photon detector
Nathan R. Gemmell,Aongus McCarthy,Baochang Liu,Michael G. Tanner,Sander N. Dorenbos,Valery Zwiller,Michael S. Patterson,Gerald S. Buller,Brian C. Wilson,Robert H. Hadfield +9 more
TL;DR: In this article, a superconducting nanowire single-photon detector was employed to record 1O2 luminescence at 1270 nm wavelength from a model photosensitizer (Rose Bengal) in solution.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
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Electron transfers in chemistry and biology
Rudolph A. Marcus,Norman Sutin +1 more
TL;DR: In this paper, the electron transfer reactions between ions and molecules in solution have been the subject of considerable experimental study during the past three decades, including charge transfer, photoelectric emission spectra, chemiluminescent electron transfer, and electron transfer through frozen media.
Journal ArticleDOI
Formation of glasses from liquids and biopolymers.
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Journal ArticleDOI
The energy landscapes and motions of proteins.
TL;DR: The concepts that emerge from studies of the conformational substates and the motions between them permit a quantitative discussion of one simple reaction, the binding of small ligands such as carbon monoxide to myoglobin.
Journal ArticleDOI
Fluorescence spectroscopy of single biomolecules.
TL;DR: The progress in applying single-molecule detection and single-Molecule spectroscopy at room temperature by laser-induced fluorescence with the use of fluorophores that are site-specifically attached to macromolecules is reviewed.