Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Posted ContentDOI
Deep learning enables rapid and robust analysis of fluorescence lifetime imaging in photon-starved conditions
Yuan-I. Chen,Yin-Jui Chang,Shih-Chu Liao,Trung D. Nguyen,Jianchen Yang,Yu-An Kuo,Soonwoo Hong,Yen-Liang Liu,H. Grady Rylander,Samantha R. Santacruz,Thomas E. Yankeelov,Hsin-Chih Yeh +11 more
TL;DR: A new deep learning-based method termed flimGANE (fluorescence lifetime imaging based on Generative Adversarial Network Estimation) that can rapidly generate accurate and high-quality FLIM images even in the photon-starved conditions is introduced.
Journal ArticleDOI
Anharmonic Molecular Motion Drives Resonance Energy Transfer in peri-Arylene Dyads.
Vladislav Sláma,Václav Perlík,Heinz Langhals,Andreas Walter,Tomáš Mančal,Jürgen Hauer,František Šanda +6 more
TL;DR: In contrast to the moderate Gaussian fluctuations of J for the parallel dyad, fluctuations for the orthogonal dyad are found to follow non-Gaussian statistics leading to significantly higher effective J in good agreement with experimental observations.
Proceedings ArticleDOI
Photon counting and timing detector modules for single-molecule spectroscopy and DNA analysis
TL;DR: In this article, a planar epitaxial single photon avalanche diode (SPAD) and monolithic active quenching and reset circuits (i-AQC) were used to achieve state-of-the-art performance.
Journal ArticleDOI
QM/MM Investigation of the Spectroscopic Properties of the Fluorophore of Bacterial Luciferase.
Germano Giuliani,Federico Melaccio,Samer Gozem,Andrea Cappelli,Massimo Olivucci,Massimo Olivucci +5 more
TL;DR: In this article, replica exchange molecular dynamics (REMD) and a hybrid ab initio multiconfigurational quantum mechanics/molecular mechanics (QM/MM) approach were employed to model the absorption and fluorescence properties of bacterial luciferin-luciferase.
Journal ArticleDOI
Stitching of tyrosine and 10H-acridin-9-one: turn-ON fluorescence in the narrow pH range 7.4–8.5 and intracellular labelling of cancer cells
TL;DR: The characteristic fluorescence change in the molecule in the pH 7.5 ± 1 range and its cell permeability allowed us to label cancer cells.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
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Journal ArticleDOI
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Journal ArticleDOI
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Journal ArticleDOI
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