Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Progress and perspectives in single-molecule optical spectroscopy.
Subhasis Adhikari,Michel Orrit +1 more
TL;DR: In this article , the progress of single-molecule optical experiments is reviewed and some perspectives for the coming years are proposed. But the focus is on methodological advances in fluorescence, super-resolution, photothermal contrast, and interferometric scattering and briefly discuss some of the applications.
Journal ArticleDOI
Historical Perspectives, State of Art and Research Trends of SPAD Arrays and Their Applications (Part II: SPAD Arrays)
Iris Cusini,David P. Berretta,Enrico Conca,Alfonso Incoronato,Francesca Madonini,Arianna Adelaide Maurina,Chiara Nonne,Simone Riccardo,Federica Villa +8 more
TL;DR: This review proposes a complete overview of silicon SPADs characteristics and applications, and focuses on the development of SPAD arrays, presenting some of the most notable examples found in literature.
Journal ArticleDOI
Confinement-Free Wide-Field Ratiometric Tracking of Single Fluorescent Molecules.
Barak Gilboa,Bo Jing,Tao Ju Cui,Maabur Sow,Anne Plochowietz,Abhishek Mazumder,Achillefs N. Kapanidis +6 more
TL;DR: The ability of SWiFi for high-throughput, ratiometric measurements of fast-diffusing species renders it a valuable tool for the single-molecule research community by bridging between confocal and TIRF detection geometries in a simple and efficient way.
Journal ArticleDOI
Simulation studies of structural changes and relaxation processes in lysozyme under pressure
TL;DR: In this article, preliminary results of a molecular dynamics simulation study on the influence of non-denaturing hydrostatic pressure on the structure and the relaxation dynamics of lysozyme were presented.
Journal ArticleDOI
Computational biotransformation of polyethylene terephthalate by depolymerase: A QM/MM approach.
Mingna Zheng,Malcolm R. DeBaun,Yanwei Li,Weiliang Dong,Shanshan Feng,Qingzhu Zhang,Wenxing Wang +6 more
TL;DR: In this paper, a detailed analysis of the biotransformation processes of PET by one of the most effective enzymes, leaf-branch compost cutinase (LCC), was systematically explored with Molecular Dynamics and Quantum Mechanics/Molecular Mechanics approaches.
References
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TL;DR: In this paper, the electron transfer reactions between ions and molecules in solution have been the subject of considerable experimental study during the past three decades, including charge transfer, photoelectric emission spectra, chemiluminescent electron transfer, and electron transfer through frozen media.
Journal ArticleDOI
Formation of glasses from liquids and biopolymers.
TL;DR: The onset of a sharp change in ddT( is the Debye-Waller factor and T is temperature) in proteins, which is controversially indentified with the glass transition in liquids, is shown to be general for glass formers and observable in computer simulations of strong and fragile ionic liquids, where it proves to be close to the experimental glass transition temperature.
Journal ArticleDOI
The energy landscapes and motions of proteins.
TL;DR: The concepts that emerge from studies of the conformational substates and the motions between them permit a quantitative discussion of one simple reaction, the binding of small ligands such as carbon monoxide to myoglobin.
Journal ArticleDOI
Fluorescence spectroscopy of single biomolecules.
TL;DR: The progress in applying single-molecule detection and single-Molecule spectroscopy at room temperature by laser-induced fluorescence with the use of fluorophores that are site-specifically attached to macromolecules is reviewed.