Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Multiscale relaxation dynamics and diffusion of myelin basic protein in solution studied by quasielastic neutron scattering
TL;DR: In this paper , high-resolution quasielastic neutron scattering spectra from Myelin Basic Protein (MBP) were analyzed at three different temperatures (283, 303, and 323 K) for a pure D2O buffer with 30% of deuterated trifluoroethanol (TFE).
Single-molecule fluorescence studies of Protein Folding and Molecular Chaperones
TL;DR: It is shown here that GroEL plays an active role in the folding of double-mutant maltose binding protein (DM-MBP), suggesting that proteins with a tendency to populate kinetically trapped intermediates require GroEL assistance for folding.
Journal ArticleDOI
Computational Insights into the Charge Relaying Properties of β-Turn Peptides in Protein Charge Transfers
TL;DR: In the β-hairpin structures, electron- or hole-binding abilities of both termini of theβ-turn motif degenerate compared with those of the two hook structures, due to the decreased macro-dipole polarity caused by the extending the two terminal strands.
Dissertation
Probing Molecular Stoichiometry by Photon Antibunching and Nanofluidics Assisted Imaging in Solution
Hao Cheng,Alexander R. Vaccaro +1 more
TL;DR: In this paper, the ability of photon antibunching to probe molecular stoichiometry in solution is explored and the underlying theoretical model is elucidated and subsequently applied to samples of different labeling stochometry.
Book ChapterDOI
Mechanostability of Virus Capsids and Their Proteins in Structure-Based Models
TL;DR: Monitoring of the subsequent refolding events in a mechanically controlled environment has also been accomplished by using single molecule optical and force spectroscopies.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Electron transfers in chemistry and biology
Rudolph A. Marcus,Norman Sutin +1 more
TL;DR: In this paper, the electron transfer reactions between ions and molecules in solution have been the subject of considerable experimental study during the past three decades, including charge transfer, photoelectric emission spectra, chemiluminescent electron transfer, and electron transfer through frozen media.
Journal ArticleDOI
Formation of glasses from liquids and biopolymers.
TL;DR: The onset of a sharp change in ddT( is the Debye-Waller factor and T is temperature) in proteins, which is controversially indentified with the glass transition in liquids, is shown to be general for glass formers and observable in computer simulations of strong and fragile ionic liquids, where it proves to be close to the experimental glass transition temperature.
Journal ArticleDOI
The energy landscapes and motions of proteins.
TL;DR: The concepts that emerge from studies of the conformational substates and the motions between them permit a quantitative discussion of one simple reaction, the binding of small ligands such as carbon monoxide to myoglobin.
Journal ArticleDOI
Fluorescence spectroscopy of single biomolecules.
TL;DR: The progress in applying single-molecule detection and single-Molecule spectroscopy at room temperature by laser-induced fluorescence with the use of fluorophores that are site-specifically attached to macromolecules is reviewed.