Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Detection of protein conformational change by optical second-harmonic generation
TL;DR: Second-harmonic generation (SHG), a surface-selective technique, offers a new means to probe structural dynamics and should serve as the starting point for more detailed studies of the mechanism of conformational change in molecules, as well as related topics such as protein folding.
Proceedings ArticleDOI
Planar silicon SPADs with 200-μm diameter and 35-ps photon timing resolution
TL;DR: In this paper, the authors developed an improved planar silicon technology for overcoming the problem and providing a solid-state alternative to MCP-PMTs in demanding TCSPC applications.
Journal ArticleDOI
Photon-HDF5: An Open File Format for Timestamp-Based Single-Molecule Fluorescence Experiments
TL;DR: Photon-HDF5 is introduced, an open and efficient file format to simplify exchange and long-term accessibility of data from single-molecule fluorescence experiments based on photon-counting detectors such as single-photon avalanche diode, photomultiplier tube, or arrays of such detectors.
Journal ArticleDOI
Evolution equation of phenotype distribution: general formulation and application to error catastrophe.
Katsuhiko Sato,Kunihiko Kaneko +1 more
TL;DR: With this formalism, it is found that the critical mutation rate is sometimes increased by the phenotypic fluctuations, i.e., noise can enhance robustness of a fitted state to mutation.
Journal ArticleDOI
Neue Wege in der Einzelmolekül‐Fluoreszenzspektroskopie: Herausforderungen für die Chemie und Einfluss auf die Biologie
Philip Tinnefeld,Markus Sauer +1 more
TL;DR: In this article, the authors discuss the Potenzial der Einzelmolekul-Fluoreszenzspektroskopie bei der Losung molekularbiologischer Probleme and diskutieren die Chancen der optischen einzel molekultechniken in Verbindung with intelligenten Markierungsstrategien, um quantitative dreidimensionale Informationen uber die dynamische Organisation in lebenden Zellen zu erhalten.
References
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Journal ArticleDOI
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Journal ArticleDOI
The energy landscapes and motions of proteins.
TL;DR: The concepts that emerge from studies of the conformational substates and the motions between them permit a quantitative discussion of one simple reaction, the binding of small ligands such as carbon monoxide to myoglobin.
Journal ArticleDOI
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