Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
Giwan Seo,Geonhee Lee,Mi-Jeong Kim,Seung Hwa Baek,Minsuk Choi,Keun Bon Ku,Chang-Seop Lee,Sangmi Jun,Daeui Park,Hong Gi Kim,Seong-Jun Kim,Jeong O. Lee,Bum Tae Kim,Edmond Changkyun Park,Seung Il Kim +14 more
TLDR
The FET sensor fabricated here is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling and is a promising FET biosensor for SARS-CoV-2.Abstract:
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.read more
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Multichannel Immunosensor Platform for the Rapid Detection of SARS-CoV-2 and Influenza A(H1N1) Virus.
Jianyong Li,Rui Lin,Yi Yang,Rongtao Zhao,Shiping Song,Yi Zhou,Jiye Shi,Lihua Wang,Hongbin Song,Hao Rongzhang +9 more
TL;DR: In this paper, a multichannel electrochemical immunoassay (MEIA) platform was developed based on a disposable screen-printed carbon electrode (SPCE) array for the on-site detection of SARS-CoV-2 and A(H1N1).
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Poly-l-Lysine-Modified Graphene Field-Effect Transistor Biosensors for Ultrasensitive Breast Cancer miRNAs and SARS-CoV-2 RNA Detection
Jianwei Gao,Chunhua Wang,Chao Wang,Yujin Chu,Shun Wang,Mingyuan Sun,Hao Ji,Yakun Gao,Yanhao Wang,Yingkuan Han,Fangteng Song,Hong Zhou Liu,Yu Zhang,Lin Han +13 more
TL;DR: An ultrasensitive poly-l-lysine (PLL)-functionalized graphene field-effect transistor (PGFET) biosensor for breast cancer miRNAs and viral RNA detection and SARS-CoV-2 RNA detection was applied, providing a promising approach for rapid cancer diagnosis and virus screening.
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Insights from nanotechnology in COVID-19: prevention, detection, therapy and immunomodulation.
Priya Singh,Deepika Singh,Pratikshya Sa,Priyanka Mohapatra,Auromira Khuntia,Sanjeeb K. Sahoo +5 more
TL;DR: In this article, a review of nano-intervention is discussed for designing effective strategies in developing advanced personal protective equipment kits, disinfectants, rapid and cost-effective diagnostics and therapeutics against the infection.
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Graphene: A Disruptive Opportunity for COVID-19 and Future Pandemics?
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TL;DR: Summary of the latest research progress on detection methods of SARS-CoV-2, providing a reference to develop rapid and accurate diagnostic strategies for COVID-19.
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