Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
Giwan Seo,Geonhee Lee,Mi-Jeong Kim,Seung Hwa Baek,Minsuk Choi,Keun Bon Ku,Chang-Seop Lee,Sangmi Jun,Daeui Park,Hong Gi Kim,Seong-Jun Kim,Jeong O. Lee,Bum Tae Kim,Edmond Changkyun Park,Seung Il Kim +14 more
TLDR
The FET sensor fabricated here is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling and is a promising FET biosensor for SARS-CoV-2.Abstract:
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.read more
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Electrochemical immunosensor with Cu 2 O nanocube coating for detection of SARS-CoV-2 spike protein.
TL;DR: In this paper, a label-free electrochemical biosensor was presented as a powerful nanobiodevice for SARS-CoV-2 spike protein detection, which was applied on a screen-printed carbon electrode modified with Cu2O nanocubes (Cu2O NCs), which provided a large surface area in a very small space.
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Rapid Point-of-Care COVID-19 Diagnosis with a Gold-Nanoarchitecture-Assisted Laser-Scribed Graphene Biosensor.
Tutku Beduk,Duygu Beduk,Jose Ilton de Oliveira Filho,Figen Zihnioglu,Candan Çiçek,Rüçhan Sertöz,Bilgin Arda,Tuncay Göksel,Kutsal Turhan,Khaled Nabil Salama,Suna Timur +10 more
TL;DR: In this article, a laser-cut graphene (LSG)-based electrochemical sensing scheme for coronavirus disease 2019 (COVID-19) diagnosis combined with three-dimensional (3D) gold nanostructures was presented.
Journal ArticleDOI
High-Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild-Type and B.1.1.7 SARS-CoV-2 in Unprocessed Saliva.
Zijie Zhang,Richa Pandey,Jiuxing Li,Jimmy Gu,Dawn White,Hannah D. Stacey,Jann C. Ang,Catherine-Jean Steinberg,Alfredo Capretta,Carlos D. M. Filipe,Karen L. Mossman,Cynthia Balion,Matthew S. Miller,Bruno J. Salena,Deborah Yamamura,Leyla Soleymani,John D. Brennan,Yingfu Li +17 more
TL;DR: In this paper, a simple and rapid saliva-based SARS-CoV-2 antigen test that utilizes a newly developed dimeric DNA aptamer, denoted as DSA1N5, that specifically recognizes the spike proteins of the wildtype virus and its Alpha and Delta variants with dissociation constants of 120, 290 and 480â
pM, respectively, and binds pseudotyped lentiviruses expressing the wild type and alpha trimeric spike proteins with affinity constants of 2.1â´µpM and 2.3â´
Journal ArticleDOI
Electrochemical Biosensor with Enhanced Antifouling Capability for COVID-19 Nucleic Acid Detection in Complex Biological Media.
TL;DR: In this paper, an antifouling electrochemical biosensor was constructed based on electropolymerized polyaniline (PANI) nanowires and newly designed peptides for the detection of the COVID-19 N-gene.
Journal ArticleDOI
MXene-Graphene Field-Effect Transistor Sensing of Influenza Virus and SARS-CoV-2
Yanxiao Li,Zhekun Peng,Natalie J. Holl,Md. Rifat Hassan,John M. Pappas,Congjie Wei,Omid Hoseini Izadi,Yang Wang,Xiangyang Dong,Cheng Wang,Yue-Wern Huang,Dong-Hyun Kim,Chenglin Wu +12 more
TL;DR: In this paper, an MXene-graphene field effect transistor (FET) sensor for both influenza virus and 2019-nCoV sensing was developed and characterized, which combines the high chemical sensitivity of MXene and the continuity of large-area high-quality graphene to form an ultra-sensitive virus-sensing transduction material (VSTM).
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