Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
Giwan Seo,Geonhee Lee,Mi-Jeong Kim,Seung Hwa Baek,Minsuk Choi,Keun Bon Ku,Chang-Seop Lee,Sangmi Jun,Daeui Park,Hong Gi Kim,Seong-Jun Kim,Jeong O. Lee,Bum Tae Kim,Edmond Changkyun Park,Seung Il Kim +14 more
TLDR
The FET sensor fabricated here is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling and is a promising FET biosensor for SARS-CoV-2.Abstract:
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.read more
Citations
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Facile biosensors for rapid detection of COVID-19.
TL;DR: This review summarises recent advances in strategies reported by international researchers and engineers concerning how to tackle COVID-19 via rapid testing, mainly through nucleic acid- and antibody testing.
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Viral Dynamics and Real-Time RT-PCR Ct Values Correlation with Disease Severity in COVID-19
Ali A. Rabaan,Raghavendra Tirupathi,Anupam A Sule,Jehad Aldali,Abbas Al Mutair,Saad Alhumaid,Muzaheed,Nitin Gupta,Thoyaja Koritala,Ramesh Adhikari,Muhammad Bilal,Manish Dhawan,Ruchi Tiwari,Saikat Mitra,Talha Bin Emran,Kuldeep Dhama +15 more
TL;DR: In this article, a review of the literature has been conducted to fill the knowledge gaps regarding the correlations between cycle threshold (Ct) values and severity/fatality rates of patients with COVID-19.
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Harikrishnan Jayamohan,Christopher J. Lambert,Himanshu J. Sant,Alex Jafek,Dhruv Patel,Haidong Feng,Michael Beeman,Tawsif Mahmood,Ugochukwu C. Nze,Bruce K. Gale +9 more
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Rapid Detection of SARS-CoV-2 Antigens Using High-Purity Semiconducting Single-Walled Carbon Nanotube-Based Field-Effect Transistors.
TL;DR: In this paper, the use of a high-purity semiconducting (sc) single-walled carbon nanotube (SWCNT)-based field effect transistor (FET) decorated with specific binding chemistry to assess the presence of SARS-CoV-2 antigens in clinical nasopharyngeal samples was reported.
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TL;DR: Advances in electrochemical biosensing platforms towards the detection of SARS-CoV-2 through studying similar viruses are reviewed.
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