Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor.
Giwan Seo,Geonhee Lee,Mi-Jeong Kim,Seung Hwa Baek,Minsuk Choi,Keun Bon Ku,Chang-Seop Lee,Sangmi Jun,Daeui Park,Hong Gi Kim,Seong-Jun Kim,Jeong O. Lee,Bum Tae Kim,Edmond Changkyun Park,Seung Il Kim +14 more
TLDR
The FET sensor fabricated here is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling and is a promising FET biosensor for SARS-CoV-2.Abstract:
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, previously called 2019-nCoV). Based on the rapid increase in the rate of human infection, the World Health Organization (WHO) has classified the COVID-19 outbreak as a pandemic. Because no specific drugs or vaccines for COVID-19 are yet available, early diagnosis and management are crucial for containing the outbreak. Here, we report a field-effect transistor (FET)-based biosensing device for detecting SARS-CoV-2 in clinical samples. The sensor was produced by coating graphene sheets of the FET with a specific antibody against SARS-CoV-2 spike protein. The performance of the sensor was determined using antigen protein, cultured virus, and nasopharyngeal swab specimens from COVID-19 patients. Our FET device could detect the SARS-CoV-2 spike protein at concentrations of 1 fg/mL in phosphate-buffered saline and 100 fg/mL clinical transport medium. In addition, the FET sensor successfully detected SARS-CoV-2 in culture medium (limit of detection [LOD]: 1.6 × 101 pfu/mL) and clinical samples (LOD: 2.42 × 102 copies/mL). Thus, we have successfully fabricated a promising FET biosensor for SARS-CoV-2; our device is a highly sensitive immunological diagnostic method for COVID-19 that requires no sample pretreatment or labeling.read more
Citations
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Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection.
Jacqueline Dinnes,Jacqueline Dinnes,Jonathan J Deeks,Ada Adriano,Sarah Berhane,Clare Davenport,Sabine Dittrich,Devy Emperador,Yemisi Takwoingi,Jane Cunningham,Sophie Beese,Janine Dretzke,Lavinia Ferrante di Ruffano,Isobel Marion Harris,Malcolm J Price,Sian Taylor-Phillips,Lotty Hooft,Mariska M.G. Leeflang,René Spijker,Ann Van den Bruel +19 more
TL;DR: Assessment of the diagnostic accuracy of point‐of‐care antigen and molecular‐based tests to determine if a person presenting in the community or in primary or secondary care has current SARS‐CoV‐2 infection found no studies at low risk of bias for all quality domains and concerns about applicability of results across all studies.
Journal ArticleDOI
Antibody tests for identification of current and past infection with SARS‐CoV‐2
Jonathan J Deeks,Jonathan J Deeks,Jacqueline Dinnes,Jacqueline Dinnes,Yemisi Takwoingi,Yemisi Takwoingi,Clare Davenport,Clare Davenport,René Spijker,René Spijker,Sian Taylor-Phillips,Sian Taylor-Phillips,Ada Adriano,Sophie Beese,Janine Dretzke,Lavinia Ferrante di Ruffano,Isobel Marion Harris,Malcolm J Price,Malcolm J Price,Sabine Dittrich,Devy Emperador,Lotty Hooft,Mariska M.G. Leeflang,Ann Van den Bruel +23 more
TL;DR: To assess the diagnostic accuracy of antibody tests to determine if a person presenting in the community or in primary or secondary care has SARS-CoV-2 infection, or has previously had SARS, and the accuracy of antibodies for use in seroprevalence surveys is assessed.
Journal ArticleDOI
Toward Nanotechnology-Enabled Approaches against the COVID-19 Pandemic.
Carsten Weiss,Marie Carrière,Laura Fusco,Laura Fusco,Ilaria Capua,Jose Angel Regla-Nava,Matteo Pasquali,James A. Scott,Flavia Vitale,Flavia Vitale,Mehmet Ünal,Cecilia Mattevi,Davide Bedognetti,Arben Merkoçi,Arben Merkoçi,Ennio Tasciotti,Ennio Tasciotti,Açelya Yilmazer,Yury Gogotsi,Francesco Stellacci,Lucia Gemma Delogu +20 more
TL;DR: Nanoimmunity by design can help to design materials for immune modulation, either stimulating or suppressing the immune response, which would find applications in the context of vaccine development for SARS-CoV-2 or in counteracting the cytokine storm, respectively.
Journal ArticleDOI
Diagnostics for SARS-CoV-2 infections.
Bhavesh D. Kevadiya,Jatin Machhi,Jonathan Herskovitz,Maxim D. Oleynikov,Wilson R. Blomberg,Neha Bajwa,Dhruvkumar Soni,Srijanee Das,Mahmudul Hasan,Milankumar Patel,Ahmed M. Senan,Santhi Gorantla,JoEllyn M McMillan,Benson J Edagwa,Robert Eisenberg,Channabasavaiah B. Gurumurthy,St Patrick Reid,Chamindie Punyadeera,Linda Chang,Howard E. Gendelman +19 more
TL;DR: A review of available and in-development diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) including nanomaterial-based tools is presented in this paper.
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Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2): a global pandemic and treatment strategies.
TL;DR: The existing treatment is essentially supportive and role of antiviral agents is yet to be established as there is no vaccination or therapy available, and this review focuses on epidemiology, symptoms, transmission, pathogenesis, ongoing available treatments and future perspectives of SARS-CoV-2.
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