REBASE—a database for DNA restriction and modification: enzymes, genes and genomes
TLDR
REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems that contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data.Abstract:
REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems. It contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data. All genomes that are completely sequenced are analyzed for RM system components, and with the advent of PacBio sequencing, the recognition sequences of DNA methyltransferases (MTases) are appearing rapidly. Thus, Type I and Type III systems can now be characterized in terms of recognition specificity merely by DNA sequencing. The contents of REBASE may be browsed from the web http://rebase.neb.com and selected compilations can be downloaded by FTP (ftp.neb.com). Monthly updates are also available via email.read more
Citations
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Naturally-Occurring Fusion Between the Regulatory and Catalytic Components of Type IIP Restriction-Modification Systems
TL;DR: In this paper, the authors present a Table of Table of Contents of the paper "Acknowledgements and acknowledgements of the authors of this paper: https://www.goprocessor.org/
Proceedings ArticleDOI
PCR-RFLP Primer Design Using Particle Swarm Optimization Combined with Chaotic Logistic Map
TL;DR: A comparison of the results obtained from PSO and CLMPSO primer design showed that CLM provided better primer sets than PSO primerDesign, and this CLM method reliably produces designs for PCR-RFLP primers which best fit the common primer constraints and also identifies available restriction enzymes.
Posted ContentDOI
SLICER: Seamless Loss of Integrated Cassettes Using Endonuclease Cleavage and Recombination in Deinococcus radiodurans
TL;DR: This work used SLICER to sequentially target four putative restriction-modification (R-M) system genes, recycling the same selective and screening markers for each subsequent deletion, resulting in a final D. radiodurans strain with 5 of the 6 putative R-M systems deleted.
Posted ContentDOI
Expression and Purification of BsaXI Restriction Endonuclease and Engineering New Specificity from BsaXI Specificity (S) Subunit
TL;DR: This work demonstrated that like Type I restriction systems, the S subunit of a Type IIB system could also be manipulated to create new specificities.
References
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