REBASE—a database for DNA restriction and modification: enzymes, genes and genomes
TLDR
REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems that contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data.Abstract:
REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems. It contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data. All genomes that are completely sequenced are analyzed for RM system components, and with the advent of PacBio sequencing, the recognition sequences of DNA methyltransferases (MTases) are appearing rapidly. Thus, Type I and Type III systems can now be characterized in terms of recognition specificity merely by DNA sequencing. The contents of REBASE may be browsed from the web http://rebase.neb.com and selected compilations can be downloaded by FTP (ftp.neb.com). Monthly updates are also available via email.read more
Citations
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Journal ArticleDOI
Analysis of Resistance to Florfenicol and the Related Mechanism of Dissemination in Different Animal-Derived Bacteria.
Peizhen Li,Tingyuan Zhu,Danying Zhou,Wei Lu,Hongmao Liu,Zhewei Sun,Jun Ying,Junwan Lu,Xi Lin,Kewei Li,Jianchao Ying,Qiyu Bao,Teng Xu +12 more
TL;DR: It is suggested that the mobile genetic elements played an important role in the replication of resistance genes and the horizontal resistance gene transfer in bacteria isolated from four farms.
Journal ArticleDOI
Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers.
TL;DR: Evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation in this species is provided.
Journal ArticleDOI
Exploring the Remarkable Diversity of Culturable Escherichia coli Phages in the Danish Wastewater Environment.
TL;DR: The isolated phages are highly diverse, estimated to represent roughly one-third of the true diversity of culturable virulent dsDNA Escherichia phages in Danish wastewater, yet almost half (40%) are not represented in metagenomic databases, emphasising the importance of isolating phages to uncover diversity.
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Toward Understanding Phage:Host Interactions in the Rumen; Complete Genome Sequences of Lytic Phages Infecting Rumen Bacteria.
Rosalind A. Gilbert,William J. Kelly,Eric Altermann,Sinead C. Leahy,Catherine M. Minchin,Diane Ouwerkerk,Athol V. Klieve +6 more
TL;DR: The first complete genome sequences of lytic phage isolates specifically infecting three genera of rumen bacteria: Bacteroides, Ruminococcus, and Streptococcus are reported, providing new genetic information and insights into how lyticphages may interact with bacteria of the rumen microbiome.
Journal ArticleDOI
PCR-RFLP analyses of formalin-fixed fish eggs for the mapping of spawning areas in the Eastern Channel and Southern North Sea
TL;DR: In this study, formalin fixation, egg conservation and DNA extraction were optimized in order to develop a specific polumerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, which was developed to distinguish eggs of cod, haddock and whiting on the one hand, and between eggs of three species of rocklings on the other.
References
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