REBASE—a database for DNA restriction and modification: enzymes, genes and genomes
TLDR
REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems that contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data.Abstract:
REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems. It contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data. All genomes that are completely sequenced are analyzed for RM system components, and with the advent of PacBio sequencing, the recognition sequences of DNA methyltransferases (MTases) are appearing rapidly. Thus, Type I and Type III systems can now be characterized in terms of recognition specificity merely by DNA sequencing. The contents of REBASE may be browsed from the web http://rebase.neb.com and selected compilations can be downloaded by FTP (ftp.neb.com). Monthly updates are also available via email.read more
Citations
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Journal ArticleDOI
Type I restriction enzymes and their relatives
TL;DR: Parts of the biochemistry, biology and regulation of Type I REases, and of the mechanisms that bacteriophages and plasmids have evolved to evade them are discussed.
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Book ChapterDOI
Natural history of eukaryotic DNA methylation systems.
TL;DR: Analysis of the domain architectures of these domains and the DNA methylases suggests that early in eukaryotic evolution they developed a close functional link with SET-domain methylases and Jumonji-related demethylases that operate on peptides in chromatin proteins.
Book ChapterDOI
Protein bioinformatics databases and resources.
TL;DR: A comprehensive review of major protein bioinformatics databases is presented (with categorization and description) in this chapter to help researchers quickly find the appropriate protein-related informatics resources.
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A versatile and efficient high-throughput cloning tool for structural biology.
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TL;DR: Fragment exchange (FX) cloning that facilitates the high-throughput generation of expression constructs is described, based on a class IIS restriction enzyme and negative selection markers, that considerably speeds up the generation ofexpression constructs compared to traditional methods and thus facilitates a broader expression screening.
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