Reclassification of Clostridium coccoides, Ruminococcus hansenii, Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus productus and Ruminococcus schinkii as Blautia coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov., Blautia producta comb. nov., Blautia schinkii comb. nov. and description of Blautia wexlerae sp. nov., isolated from human faeces.
TLDR
Phenotypic and phylogenetic studies were performed on 15 isolates of an unidentified Gram-positive, anaerobic, non-sporulating coccobacillus-shaped bacterium isolated from human faeces, and it is suggested that a novel genus, Blautia gen. nov., is proposed.Abstract:
Phenotypic and phylogenetic studies were performed on 15 isolates of an unidentified Gram-positive, anaerobic, non-sporulating coccobacillus-shaped bacterium isolated from human faeces. The novel organisms were catalase-negative, indole-negative and produced acetate and succinate as end products of metabolism. Comparative 16S rRNA gene sequencing demonstrated that the 15 isolates were highly related to each other and formed a hitherto unknown subline within the clostridial rRNA cluster XIVa. The novel isolates formed a robust phylogenetic group with a number of organisms which included Clostridium coccoides, Ruminococcus luti, Ruminococcus obeum and a number of other misclassified ruminococci. On the basis of these studies, a novel genus, Blautia gen. nov., is proposed. It is suggested that Clostridium coccoides, Ruminococcus hansenii, Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus productus, and Ruminococcus schinkii are transferred to this genus as Blautia coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov., Blautia producta comb. nov. and Blautia schinkii comb. nov. One of the new isolates, the hitherto unknown coccus-shaped bacterial strain WAL 14507T (=ATCC BAA-1564T=DSM 19850T) is proposed as representing the type strain of a novel species, Blautia wexlerae sp. nov.read more
Citations
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Global and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome
Mirjana Rajilić–Stojanović,Mirjana Rajilić–Stojanović,Elena Biagi,Hans G.H.J. Heilig,Kajsa Kajander,Riina A. Kekkonen,Sebastian Tims,Willem M. de Vos,Willem M. de Vos +8 more
TL;DR: Global and deep molecular analysis of fecal samples indicates that patients with IBS have a different composition of microbiota, and correlation analysis of the microbial groups indicated the involvement of several groups of Firmicutes and Proteobacteria in the pathogenesis of IBS.
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The first 1000 cultured species of the human gastrointestinal microbiota
TL;DR: This review provides a systematic overview and detailed references of the total of 1057 intestinal species of Eukarya, Archaea and Bacteria based on the phylogenetic framework of their small subunit ribosomal RNA gene sequences and unifies knowledge about the prevalence, abundance, stability, physiology, genetics and the association with human health of these gastrointestinal microorganisms.
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Phylogenetic distribution of three pathways for propionate production within the human gut microbiota
Nicole Reichardt,Sylvia H. Duncan,Pauline Young,Álvaro Belenguer,Carol McWilliam Leitch,Karen P. Scott,Harry J. Flint,Petra Louis +7 more
TL;DR: A better understanding of the microbial ecology of short-chain fatty acid formation may allow modulation of propionate formation by the human gut microbiota.
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Bile acid is a host factor that regulates the composition of the cecal microbiota in rats
K.B.M. Saiful Islam,Satoru Fukiya,Masahito Hagio,Nobuyuki Fujii,Satoshi Ishizuka,Tadasuke Ooka,Yoshitoshi Ogura,Tetsuya Hayashi,Atsushi Yokota +8 more
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TL;DR: Current knowledge of how macronutrient metabolism by the gut microbiome influences human health is summarized and knowledge gaps that could contribute to the understanding of overall human wellness will be identified.
References
More filters
Journal ArticleDOI
Improved tools for biological sequence comparison.
TL;DR: Three computer programs for comparisons of protein and DNA sequences can be used to search sequence data bases, evaluate similarity scores, and identify periodic structures based on local sequence similarity.
Journal ArticleDOI
ARB: a software environment for sequence data
Wolfgang Ludwig,Oliver Strunk,Ralf Westram,Lothar Richter,Harald Meier,Yadhukumar,Arno Buchner,Tina Lai,Susanne Steppi,Gangolf Jobb,Wolfram Förster,Igor Brettske,Stefan Gerber,Anton W. Ginhart,Oliver Gross,Silke Grumann,Stefan Hermann,Ralf Jost,Andreas König,Thomas Liss,Ralph Lüßmann,Michael May,Björn Nonhoff,Boris Reichel,Robert Strehlow,Alexandros Stamatakis,Norbert Stuckmann,Alexander Vilbig,Michael Lenke,Thomas Ludwig,Arndt Bode,Karl-Heinz Schleifer +31 more
TL;DR: The ARB program package comprises a variety of directly interacting software tools for sequence database maintenance and analysis which are controlled by a common graphical user interface.
Journal ArticleDOI
Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology
Erko Stackebrandt,B. M. Goebel +1 more
TL;DR: Amorphous metal alloys are employed in acoustic devices dependent upon the properties of low acoustic velocity and low attenuation, such as wire, strip and bulk delay lines.
Journal ArticleDOI
Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
TL;DR: The complete nucleotide sequence of the 16S RNA gene from the rrnB cistron of Escherichia coli has been determined by using three rapid DNA sequencing methods, and discrepancies may be explained by heterogeneity among 16S rRNA sequences from different cistrons.
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