Showing papers by "University of Antananarivo published in 2017"

Agro-ecological functions of crop residues under conservation agriculture. A review

Abstract: Conservation agriculture, which is based on minimum tillage, permanent soil cover and crop rotations, has widely been promoted as a practice to maintain or improve soil quality and enhance crop productivity. To a large extent, the beneficial effects of conservation agriculture are expected to be provided by permanent soil cover with crop residues. Surface crop residues play an important role for crop growth through their benefits on soil-related structural components and processes in the agro-ecosystem, referred to in this study as agro-ecological functions. Through a meta-analysis of the literature, we have studied the relative effects of surface crop residue levels on the performance of a set of agro-ecological functions compared with a no-till bare soil, i.e., without surface residues. The selected agro-ecological functions were soil water evaporation control, soil water infiltration, soil water runoff control, soil loss control, soil nutrient availability, soil organic carbon (SOC) stocks and gains, weed control and soil meso- and macrofauna abundance. The potential effects of crop residue cover were quantified using boundary line models. Our main findings were (1) 8 t ha−1 of residues were needed to decrease soil water evaporation by about 30% compared to no-till bare soil. (2) To achieve the maximum effect on soil water infiltration, water runoff and soil loss control, residue amounts of at least 2 t ha−1 were required. (3) The effect of increasing the amounts of surface crop residues on soil nutrient supply (N, P and K) was relatively low; the boundary line models were not significant. (4) The average annual SOC gain increased with increasing amounts of residues, with a mean of 0.38 t C ha−1 year−1 with 4 to 5 t ha−1 of residues. (5) Weed emergence and biomass can be reduced by 50% compared to a no-till bare soil with residue amounts of 1 t ha−1 or more. (6) There was a weak response in soil meso- and macrofauna abundance to increasing amounts of surface crop residues. The maximum effect corresponded to an increase of 45% compared to a no-till bare soil and was reached from 10 t ha−1 of residues. Our findings suggest that optimal amounts of surface residues in the practice of conservation agriculture will largely depend on the type of constraints to crop production which can be addressed with mulching.

Genomic landscape of human diversity across Madagascar.

Abstract: Although situated ∼400 km from the east coast of Africa, Madagascar exhibits cultural, linguistic, and genetic traits from both Southeast Asia and Eastern Africa. The settlement history remains contentious; we therefore used a grid-based approach to sample at high resolution the genomic diversity (including maternal lineages, paternal lineages, and genome-wide data) across 257 villages and 2,704 Malagasy individuals. We find a common Bantu and Austronesian descent for all Malagasy individuals with a limited paternal contribution from Europe and the Middle East. Admixture and demographic growth happened recently, suggesting a rapid settlement of Madagascar during the last millennium. However, the distribution of African and Asian ancestry across the island reveals that the admixture was sex biased and happened heterogeneously across Madagascar, suggesting independent colonization of Madagascar from Africa and Asia rather than settlement by an already admixed population. In addition, there are geographic influences on the present genomic diversity, independent of the admixture, showing that a few centuries is sufficient to produce detectable genetic structure in human populations.

Host Ecology Rather Than Host Phylogeny Drives Amphibian Skin Microbial Community Structure in the Biodiversity Hotspot of Madagascar.

Abstract: Host-associated microbiotas of vertebrates are diverse and complex communities that contribute to host health. In particular, for amphibians, cutaneous microbial communities likely play a significant role in pathogen defense; however, our ecological understanding of these communities is still in its infancy. Here we take advantage of the fully endemic and locally species-rich amphibian fauna of Madagascar to investigate the factors structuring amphibian skin microbiota on a large scale. Using amplicon-based sequencing, we evaluate how multiple host species traits and site factors affect host bacterial diversity and community structure. Madagascar is home to over 400 native frog species, all of which are endemic to the island; more than 100 different species are known to occur in sympatry within multiple rainforest sites. We intensively sampled frog skin bacterial communities, from over 800 amphibians from 89 species across 30 sites in Madagascar during three field visits, and found that skin bacterial communities differed strongly from those of the surrounding environment. Richness of bacterial operational taxonomic units (OTUs) and phylogenetic diversity differed among host ecomorphs, with arboreal frogs exhibiting lower richness and diversity than terrestrial and aquatic frogs. Host ecomorphology was the strongest factor influencing microbial community structure, with host phylogeny and site parameters (latitude and elevation) explaining less but significant portions of the observed variation. Correlation analysis and topological congruency analyses revealed little to no phylosymbiosis for amphibian skin microbiota. Despite the observed geographic variation and low phylosymbiosis, we found particular OTUs that were differentially abundant between particular ecomorphs. For example, the genus Pigmentiphaga (Alcaligenaceae) was significantly enriched on arboreal frogs, Methylotenera (Methylophilaceae) was enriched on aquatic frogs, and Agrobacterium (Rhizobiaceae) was enriched on terrestrial frogs. The presence of shared bacterial OTUs across geographic regions for selected host genera suggests the presence of core microbial communities which in Madagascar, might be driven more strongly by a species’ preference for specific microhabitats than by the physical, physiological or biochemical properties of their skin. These results corroborate that both host and environmental factors are driving community assembly of amphibian cutaneous microbial communities, and provide an improved foundation for elucidating their role in disease resistance.

Pathways to transformational change in the face of climate impacts: an analytical framework

Abstract: Unprecedented impacts of climate change and climate variability in the twenty-first century are likely to require transformational social, organizational and human responses. Yet, little existing empirical work examines how decision-makers can facilitate such responses. This paper suggests that in order to assess whether responses to climate risks and threats are transformational, it is necessary to move away from a focus only on outcomes and scale and towards the multiple dimensions of social responses and the processes through which transformational changes are realized. In so doing, the paper seeks to move the discussion on transformational change towards the processes and sustainability of adaptation interventions, and the changes they trigger. Drawing on the literature on transformational change in organizational theory and social–ecological systems, the paper first develops a framework with which to examine and assess development and adaptation interventions. The framework is then applied to eight i...

The Mouse Lemur, a Genetic Model Organism for Primate Biology, Behavior, and Health

Abstract: Systematic genetic studies of a handful of diverse organisms over the past 50 years have transformed our understanding of biology. However, many aspects of primate biology, behavior, and disease are absent or poorly modeled in any of the current genetic model organisms including mice. We surveyed the animal kingdom to find other animals with advantages similar to mice that might better exemplify primate biology, and identified mouse lemurs (Microcebus spp.) as the outstanding candidate. Mouse lemurs are prosimian primates, roughly half the genetic distance between mice and humans. They are the smallest, fastest developing, and among the most prolific and abundant primates in the world, distributed throughout the island of Madagascar, many in separate breeding populations due to habitat destruction. Their physiology, behavior, and phylogeny have been studied for decades in laboratory colonies in Europe and in field studies in Malagasy rainforests, and a high quality reference genome sequence has recently been completed. To initiate a classical genetic approach, we developed a deep phenotyping protocol and have screened hundreds of laboratory and wild mouse lemurs for interesting phenotypes and begun mapping the underlying mutations, in collaboration with leading mouse lemur biologists. We also seek to establish a mouse lemur gene “knockout” library by sequencing the genomes of thousands of mouse lemurs to identify null alleles in most genes from the large pool of natural genetic variants. As part of this effort, we have begun a citizen science project in which students across Madagascar explore the remarkable biology around their schools, including longitudinal studies of the local mouse lemurs. We hope this work spawns a new model organism and cultivates a deep genetic understanding of primate biology and health. We also hope it establishes a new and ethical method of genetics that bridges biological, behavioral, medical, and conservation disciplines, while providing an example of how hands-on science education can help transform developing countries.

Reconstructing Asian faunal introductions to eastern Africa from multi-proxy biomolecular and archaeological datasets

Abstract: Human-mediated biological exchange has had global social and ecological impacts. In sub-Saharan Africa, several domestic and commensal animals were introduced from Asia in the pre-modern period; however, the timing and nature of these introductions remain contentious. One model supports introduction to the eastern African coast after the mid-first millennium CE, while another posits introduction dating back to 3000 BCE. These distinct scenarios have implications for understanding the emergence of long-distance maritime connectivity, and the ecological and economic impacts of introduced species. Resolution of this longstanding debate requires new efforts, given the lack of well-dated fauna from high-precision excavations, and ambiguous osteomorphological identifications. We analysed faunal remains from 22 eastern African sites spanning a wide geographic and chronological range, and applied biomolecular techniques to confirm identifications of two Asian taxa: domestic chicken (Gallus gallus) and black rat (Rattus rattus). Our approach included ancient DNA (aDNA) analysis aided by BLAST-based bioinformatics, Zooarchaeology by Mass Spectrometry (ZooMS) collagen fingerprinting, and direct AMS (accelerator mass spectrometry) radiocarbon dating. Our results support a late, mid-first millennium CE introduction of these species. We discuss the implications of our findings for models of biological exchange, and emphasize the applicability of our approach to tropical areas with poor bone preservation.

The sweet and the bitter: Intertwined positive and negative social impacts of a biodiversity offset

Abstract: Major developments, such as mines, will often have unavoidable environmental impacts. In such cases, investors, governments, or even a company's own standards increasingly require implementation of biodiversity offsets (investment in conservation with a measurable outcome) with the aim of achieving 'no net loss' or even a 'net gain' of biodiversity. Where conservation is achieved by changing the behaviour of people directly using natural resources, the offset might be expected to have social impacts but such impacts have received very little attention. Using the case study of Ambatovy, a major nickel mine in the eastern rainforests of Madagascar and a company at the vanguard of developing biodiversity offsets, we explore local perceptions of the magnitude and distribution of impacts of the biodiversity offset project on local wellbeing. We used both qualitative (key informant interviews and focus group discussions) and quantitative (household survey) methods. We found that the biodiversity offsets, which comprise both conservation restrictions and development activities, influenced wellbeing in a mixture of positive and negative ways. However, overall, respondents felt that they had suffered a net cost from the biodiversity offset. It is a matter of concern that benefits from development activities do not compensate for the costs of the conservation restrictions, that those who bear the costs are not the same people as those who benefit, and that there is a mismatch in timing between the immediate restrictions and the associated development activities which take some time to deliver benefits. These issues matter both from the perspective of environmental justice, and for the long-term sustainability of the biodiversity benefits the offset is supposed to deliver.

Mineralogical analysis of ferralitic soils in Madagascar using NIR spectroscopy

Abstract: The applicability of near-infrared reflectance spectroscopy (NIRS) as tool for estimating mineral soil constituents was assessed over a wide range of highly weathered soils in Madagascar. The predictions were based on two types of methods, chemometric methods using multivariate calibration models from partial least squares (PLS) regressions, and the use of spectral signatures of specific minerals. The predictions of mineralogical properties of soils using chemometric methods were poor, except for the quantification of iron oxides extracted with citrate-bicarbonate dithionite (CBD) (R2cv = 0.80). Soil minerals (kaolinite, gibbsite, goethite and hematite) were also estimated by NIRS but with less accuracy (R2cv = 0.50–0.80). The predictions of kaolinite and gibbsite contents were improved by the use of the peak intensity of the first derivative spectra, situated at around 2205 nm for kaolinite and 2265 nm for gibbsite. The results indicate that NIRS can be used as a rapid analytical technique to simultaneously estimate the main minerals of highly weathered ferralitic soils with acceptable accuracy.

Estimating Herd Immunity to Amphibian Chytridiomycosis in Madagascar Based on the Defensive Function of Amphibian Skin Bacteria

Abstract: For decades, Amphibians have been globally threatened by the still expanding infectious disease, chytridiomycosis. Madagascar is an amphibian biodiversity hotspot where Batrachochytrium dendrobatidis (Bd) has only recently been detected. While no Bd-associated population declines have been reported, the risk of declines is high when invasive virulent lineages become involved. Cutaneous bacteria contribute to host innate immunity by providing defense against pathogens for numerous animals, including amphibians. Little is known, however, about the cutaneous bacterial residents of Malagasy amphibians and the functional capacity they have against Bd. We cultured 3179 skin bacterial isolates from over 90 frog species across Madagascar, identified them via Sanger sequencing of approximately 700 bp of the 16S rRNA gene, and characterized their functional capacity against Bd. A subset of isolates was also tested against multiple Bd genotypes. In addition, we applied the concept of herd immunity to estimate Bd-associated risk for amphibian communities across Madagascar based on bacterial antifungal activity. We found that multiple bacterial isolates (39% of all isolates) cultured from the skin of Malagasy frogs were able to inhibit Bd. Mean inhibition was weakly correlated with bacterial phylogeny, and certain taxonomic groups appear to have a high proportion of inhibitory isolates, such as the Enterobacteriaceae, Pseudomonadaceae, and Xanthamonadaceae (84, 80, and 75% respectively). Functional capacity of bacteria against Bd varied among Bd genotypes; however, there were some bacteria that showed broad spectrum inhibition against all tested Bd genotypes, suggesting that these bacteria would be good candidates for probiotic therapies. We estimated Bd-associated risk for sampled amphibian communities based on the concept of herd immunity. Multiple amphibian communities, including those in the amphibian diversity hotspots, Andasibe and Ranomafana, were estimated to be below the 80% herd immunity threshold, suggesting they may be at higher risk to chytridiomycosis if a lethal Bd genotype emerges in Madagascar. While this predictive approach rests on multiple assumptions, and incorporates only one component of hosts' defense against Bd, their culturable cutaneous bacterial defense, it can serve as a foundation for continued research on Bd-associated risk for the endemic frogs of Madagascar.

Durability monitoring of long-lasting insecticidal (mosquito) nets (LLINs) in Madagascar: physical integrity and insecticidal activity.

Abstract: Long-lasting insecticidal mosquito nets (LLINs) are highly effective for malaria prevention. However, it is also clear that durability monitoring is essential to predict when, post-distribution, a net population, no longer meets minimum WHO standards and needs to be replaced. Following a national distribution campaign in 2013, we tracked two durability indicators, physical integrity and bio-efficacy at six and 12 months post-distribution. While the loss of net integrity during this period was in line with expectations for a one-year net life, bio-efficacy results suggested that nets were losing insecticidal effect faster than expected. The rate of bio-efficacy loss varied significantly between different net brands. We tested 600 randomly selected LLINs, 200 from each of three net brands. Each brand came from different eco-epidemiological zones reflecting the original distribution scheme. Fabric integrity (size and number of holes) was quantified using the proportional hole index (pHI). A subsample of the nets, 134 new nets, 150 at six months and 124 at 12 months, were then tested for bio-efficacy using the World Health Organization (WHO) recommended method. Three net types, Netprotect®, Royalsentry® and Yorkool®, were followed. After six months, 54%, 39% and 45%, respectively, showed visible loss of integrity. The median pHI by type was estimated to be one, zero and one respectively. The percentage of damaged nets increased after 12 months such that 83.5%, 74% and 68.5%, had holes. The median pHI for each brand of nets was 47.5, 47 and 23. No significant difference in the estimated pHI at either six or 12 months was observed. There was a statistically significant difference in the proportion of hole size category between the three brands (χ 2 = 15.761, df = 4, P = 0.003). In cone bio-assays, mortality of new Yorkool® nets was surprisingly low (48.6%), mortality was 90.2% and 91.3% for Netprotect® and Royalsentry® (F (2, 131) = 81.59, P < 0.0001), respectively. At 12 month use, all tested nets were below the WHO threshold for replacement. These findings suggest that there is a need for better net quality control before distribution. More frequent replacement of LLINs is probably not an option programmatically. Regardless of prior approval, LLIN durability monitoring for quality assessment as well as net loss following distribution is necessary to improve malaria control efforts.

Human translocation as an alternative hypothesis to explain the presence of giant tortoises on remote islands in the south‐western Indian Ocean

Abstract: Giant tortoises are known from several remote islands in the Indian Ocean (IO). Our present understanding of ocean circulation patterns, the age of the islands, and the life history traits of giant tortoises makes it difficult to comprehend how these animals arrived on such small, remote and geologically young (8–1.5 Ma) landmasses. For colonization to have occurred by dispersal, giant tortoises must either have originated in Madagascar or Africa and swum for hundreds of km against the ocean currents, or have launched themselves from the eastern IO margin and drifted with the currents over several thousands of km of open ocean. After these navigational feats, the tortoises would have needed to found new, viable populations on potentially inhospitable volcanic or coral outcrops. Geologically recent sea level changes are likely to have eliminated terrestrial life from islands like Aldabra, complicating the scenario. We reviewed information relating to IO geology, the evolution and ecology of giant tortoises, and the spread of humans within the region, and propose an alternative explanation: we posit that giant tortoises were introduced to the IO islands by early Austronesian sailors, possibly to establish provisioning stations for their journeys, just as European sailors did in more recent historical times.

The most used medicinal plants by communities in Mahaboboka, Amboronabo, Mikoboka, Southwestern Madagascar

Abstract: This paper reports a study undertaken in three remote communities (Mahaboboka, Amboronabo, Mikoboka), located in Sakaraha, Southwestern Madagascar. Not only villages are far away from sanitary infrastructures and doctors but drugs and consulting fees are unaffordable to villagers. They rely essentially on natural resources for health care as for most of rural areas in Madagascar. This paper aims to document medicinal plants used by communities in Sakaraha and to present the most important plant species used in traditional medicine. Semi – structured interview was conducted within 214 informants in 34 villages of the study area. Different ailments encountered in the site study were classified in various categories. For data analysis, frequency of citation (Fq), Informant Consensus Factor (Fic), Fidelity Level (FL) and Use Value (UV) were assessed to find agreement among informants about the use of plants as remedies. Mann-Whitney, Kruskall-Wallis and Spearman correlation tests were performed to determine use of medicinal plants following social status of informants. A total of 235 medicinal plant species belonging to 198 genera and 75 families were inventoried. The richest families in species used for medicinal purposes were: Fabaceae, Apocynaceae, Rubiaceae, Euphorbiaceae, Asteraceae, and Poaceae. Plant species cited by informants were used to treat 76 various ailments classified in 13 categories. Leaves and leafy twigs were the most used plant parts and decoction was the mostly cited way of preparation of these medicinal plants species. In average, local people cited 6.7 ± 6.03 medicinal taxa among them, Cedrelopsis grevei is the most cited medicinal plants (Fq. 0.28). With Cedrelopsis grevei (UV = 0.48), Henonia scoparia (UV = 0.43) are mostly used species. Leonotis nepetifolia (FL = 96%) and Strychnos henningsii (FL = 92%) are plant species claimed by high percentage of informants to treat the Digestive System Disorder. This study highlighted that medicinal plants used by people from three communities in the Southwestern Madagascar are diverse. These plants species ensure care to all family members including babies, children, mothers and adult people. Through this study, newly reported medicinal plants were identified for further work.

Crustal structure of southern Madagascar from receiver functions and ambient noise correlation: Implications for crustal evolution

Abstract: The Precambrian rocks of Madagascar were formed and/or modified during continental collision known as the Pan-African orogeny. Aborted Permo-Triassic Karoo rifting and the subsequent separation from Africa and India resulted in the formation of sedimentary basins in the west and volcanic activity predominantly along the margins. Many geological studies have documented the imprint of these processes, but little was known about the deeper structure. We therefore deployed seismic stations along an SE-NW trending profile spanning nearly all geological domains of southern Madagascar. Here, we focus on the crustal structure, which we determined based on joint analysis of receiver functions and surface waves derived from ambient noise measurements. For the sedimentary basin we document a thinning of the underlying crystalline basement by up to ∼60% to 13 km. The crustal velocity structure demonstrates that the thinning was accomplished by removal or exhumation of the lower crust. Both the Proterozoic and Archean crust have a 10 km thick upper crust and 10-12 km thick midcrust. However, in contrast to the typical structure of Proterozoic and Archean aged crust, the Archean lower crust is thicker and faster than the Proterozoic one, indicating possible magmatic intrusions; an underplated layer of 2-8 km thickness is present only below the Archean crust. The Proterozoic mafic lower crust might have been lost during continental collision by delamination or subduction, or thinned as a result of extensional collapse. Finally, the Cretaceous volcanics along the east coast are characterized by thin crust (30 km) and very large VP/VS ratios.

An intra-population analysis of the indris' song dissimilarity in the light of genetic distance.

Abstract: The increasing interest in the evolution of human language has led several fields of research to focus on primate vocal communication. The ‘singing primates’, which produce elaborated and complex sequences of vocalizations, are of particular interest for this topic. Indris (Indri indri) are the only singing lemurs and emit songs whose most distinctive portions are “descending phrases” consisting of 2-5 units. We examined how the structure of the indris’ phrases varied with genetic relatedness among individuals. We tested whether the acoustic structure could provide conspecifics with information about individual identity and group membership. When analyzing phrase dissimilarity and genetic distance of both sexes, we found significant results for males but not for females. We found that similarity of male song-phrases correlates with kin in both time and frequency parameters, while, for females, this information is encoded only in the frequency of a single type. Song phrases have consistent individual-specific features, but we did not find any potential for advertising group membership. We emphasize the fact that genetic and social factors may play a role in the acoustic plasticity of female indris. Altogether, these findings open a new perspective for future research on the possibility of vocal production learning in these primates.

Remote sensing combined with social-ecological data: The importance of diverse land uses for ecosystem service provision in north-eastern Madagascar

Abstract: Through ongoing deforestation in the tropics, forest-related ecosystem services are declining, while ecosystem services provided by agricultural land uses are on the increase. Land system science provides a framework for analysing the links between land use change and the resulting socio-environmental trade-offs. However, the evidence base to support the navigation of such trade-offs is often lacking, as information on land use cannot directly be obtained through remote sensing and census data is often unavailable at sufficient spatial resolution. The global biodiversity hotspot of north-eastern Madagascar exemplifies these challenges. Combining land use data obtained through remote sensing with social-ecological data from a regional level household survey, we attempt to make the links between land use and ecosystem service benefits explicit. Our study confirmed that remotely sensed information on landscapes reflects households’ involvement in rice production systems. We further characterized landscapes in terms of “ecosystem service bundles” linked to specific land uses, as well as in terms of ecosystem service benefits to households. The map of landscape types could help direct future conservation and development efforts towards places where there is potential for success.

Defining the next generation of Plasmodium vivax diagnostic tests for control and elimination: Target product profiles

Abstract: The global prevalence of malaria has decreased over the past fifteen years, but similar gains have not been realized against Plasmodium vivax because this species is less responsive to conventional malaria control interventions aimed principally at P. falciparum. Approximately half of all malaria cases outside of Africa are caused by P. vivax. This species places dormant forms in human liver that cause repeated clinical attacks without involving another mosquito bite. The diagnosis of acute patent P. vivax malaria relies primarily on light microscopy. Specific rapid diagnostic tests exist but typically perform relatively poorly compared to those for P. falciparum. Better diagnostic tests are needed for P. vivax. To guide their development, FIND, in collaboration with P. vivax experts, identified the specific diagnostic needs associated with this species and defined a series of three distinct target product profiles, each aimed at a particular diagnostic application: (i) point-of-care of acutely ill patients for clinical care purposes; (ii) point-of-care asymptomatic and otherwise sub-patent residents for public health purposes, e.g., mass screen and treat campaigns; and (iii) ultra-sensitive not point-of-care diagnosis for epidemiological research/surveillance purposes. This report presents and discusses the rationale for these P. vivax-specific diagnostic target product profiles. These contribute to the rational development of fit-for-purpose diagnostic tests suitable for the clinical management, control and elimination of P. vivax malaria.

Gecko phylogeography in the Western Indian Ocean region: the oldest clade of Ebenavia inunguis lives on the youngest island

Abstract: Aim We studied the gecko genus Ebenavia to reconstruct its colonization history, test for anthropogenic versus natural dispersal out of Madagascar, and correlate divergence date estimates of our phylogeny with geological age estimates of islands in the region. Location Madagascar and surrounding islands of the Western Indian Ocean (Comoros, Mayotte, Mauritius, Pemba). Methods We reconstructed the phylogeny of Ebenavia covering its entire geographical range using a molecular data set of three mitochondrial and two nuclear markers. We estimated divergence times based on calibrations using (1) previously calculated mutation rates of mitochondrial markers, (2) a combination of these rates with old or (3) young geological age estimates for some of the islands inhabited by the genus, and (4) an independent data set with fossil outgroup calibration points. Results Ebenavia inunguis, one of two recognized species of the genus, comprises multiple ancient evolutionary lineages. The earliest divergence within this complex (Miocene, 13–20 Ma; 95% credibility interval [CI]: 4–29 Ma) separates the population of the Comoros Islands, excluding Mayotte, from all other lineages. The age estimates for island lineages coincide with the geological age estimates of the islands except for Grand Comoro, where the age of the local clade (3–5 Ma; 95% CI: 2–7 Ma) significantly predates the estimated island age (0·5 Ma). A clade from north Madagascar + Mayotte + Pemba is estimated to have diverged from an eastern Malagasy clade in the Miocene. Main Conclusions Our results suggest that Grand Comoro Island is geologically older than previously estimated. The islands of the Comoros and Pemba were probably colonized via natural dispersal out of Madagascar (> 1000 km in the case of Pemba). Mauritius was most likely colonized only recently from eastern Madagascar via human translocation.

Interactions Between Carnivores in Madagascar and the Risk of Disease Transmission

Abstract: Introduced carnivores exert considerable pressure on native predators through predation, competition and disease transmission. Recent research shows that exotic carnivores negatively affect the distribution and abundance of the native and endangered carnivores of Madagascar. In this study, we provide information about the frequency and distribution of interactions between exotic (dogs and cats) and native carnivores (Eupleridae) in the Betampona Natural Reserve (BNR), Madagascar, using noninvasive camera trap surveys. Domestic dogs (Canis familiaris) were the most frequently detected carnivore species within the BNR, and we found that indirect interactions between exotic and native carnivores were frequent (n = 236). Indirect interactions were more likely to occur near the research station (incidence rate ratio = 0.91), which may constitute a disease transmission hot spot for carnivores at BNR. The intervals between capture of native and exotic carnivores suggest that there is potential for pathogen transmission between species in BNR. These capture intervals were significantly shorter near the edge of the reserve (P = 0.04). These data could be used to implement biosecurity measures to monitor interactions and prevent disease transmission between species at the domestic animal and wildlife interface.

Monitoring internet trade to inform species conservation actions

Abstract: Specimens, parts and products of threatened species are commonly traded on the internet. This could threaten the survival of some wild populations. We outline 2 methods to monitor internet sales of species to assess potential threats and inform conservation actions. Our first method combines systematic monitoring of online offers of plants for sale with expert consultation. Our second method utilises a computational model, trained to expert-classified records using probabilistic inference, to predict unknown properties of the traded taxa. We used these methods to monitor internet trade in 5 genera of succulent plant species endemic to Madagascar, some of which have recently been listed for trade regulation under the Convention on International Trade in Endangered Species (CITES). This revealed potential threats to wild populations: for instance, almost all species recorded were of high conservation concern, yet most offers for live plants were of apparently wild-collected specimens (85%). Our model predicted with 89% accuracy whether the plants were classified as propagated or wild collected by an expert, although accuracy dropped for data collected in the following summer. Our results highlight potential threats by internet trade to the survival of some CITES and non-CITES listed plant species from Madagascar. These should be addressed by further conservation actions and policy. More generally, our results reveal how standardised internet surveys can provide information on levels of trade in wild-collected threatened species that could impact on natural populations, and can provide data that can be incorporated into models to facilitate future monitoring and enforcement.