University of Western Australia

About: University of Western Australia is a education organization based out in Perth, Western Australia, Australia. It is known for research contribution in the topics: Population & Poison control. The organization has 29613 authors who have published 87405 publications receiving 3064466 citations. The organization is also known as: UWA & University of WA.

Porcine small intestine submucosa (SIS) is not an acellular collagenous matrix and contains porcine DNA: Possible implications in human implantation

Abstract: Porcine small intestinal submucosa (SIS) has been recommended as a cell-free, biocompatible biomaterial for the repair of rotator cuff tendon tear. However, we have observed noninfectious edema and severe pain in patients who have undergone SIS implantation for tendon repair. The aim of this study was to conduct an independent assessment of the safety and efficacy of Restore SIS membrane. The Restore orthobiologic implant was examined by histology and the nested PCR technique using porcine immunoreceptor DAP12 gene to examine if SIS membrane contained porcine cells or DNA, respectively. The material was also implanted into mice and rabbits for the evaluation of biological reaction and inflammatory response. Restore SIS was found to contain multiple layers of porcine cells. Chloroacetate esterase staining showed that some of these cells were mast cells. Nested PCR of the DAP12 gene demonstrated that Restore SIS contained porcine DNA material. Subcutaneous implantation of Restore SIS membrane in mice, and in rabbits for rotator cuff tendon repair, showed that the membrane caused an inflammatory reaction characterized by massive lymphocyte infiltration. In conclusion, Restore SIS is not an acellular collagenous matrix, and contains porcine DNA. Our results contradict the current view that Restore SIS is a cell-free biomaterial, and that no inflammatory response is elicited by its implantation. We suggest that further studies should be conducted to evaluate the clinical safety and efficacy of SIS implant biomaterials.

Managing the whole landscape: historical, hybrid, and novel ecosystems

Abstract: The reality confronting ecosystem managers today is one of heterogeneous, rapidly transforming landscapes, particularly in the areas more affected by urban and agricultural development. A landscape management framework that incorporates all systems, across the spectrum of degrees of alteration, provides a fuller set of options for how and when to intervene, uses limited resources more effectively, and increases the chances of achieving management goals. That many ecosystems have departed so substantially from their historical trajectory that they defy conventional restoration is not in dispute. Acknowledging novel ecosystems need not constitute a threat to existing policy and management approaches. Rather, the development of an integrated approach to management interventions can provide options that are in tune with the current reality of rapid ecosystem change.

Differential Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Vascular Reactivity of the Forearm Microcirculation in Hyperlipidemic, Overweight Men

Abstract: Background—Recent evidence supports differential effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), the 2 major ω3 fatty acids of marine origin, on blood pressure in humans and vascular reactivity in adult spontaneously hypertensive rats. We investigated possible differences in the effects of purified EPA or DHA on forearm vascular reactivity in overweight hyperlipidemic men that might contribute to the blood pressure–lowering effects of fish oils. Methods and Results—With a double-blind, placebo-controlled trial of parallel design, 59 overweight, mildly hyperlipidemic men were randomized to receive 4 g/d purified EPA, DHA, or olive oil (placebo) capsules while continuing their usual diets for 6 weeks. Forearm blood flow (FBF) was measured with venous occlusion, strain-gauge plethysmography during the sequential intra-arterial administration of acetylcholine (7.5, 15, and 30 μg/min), sodium nitroprusside (1.5, 3, and 10 μg/min), norepinephrine (10, 20, and 40 ng/min), a single-dose inf...

The crustal continuum model for late-Archaean lode-gold deposits of the Yilgarn Block, Western Australia

Abstract: Most Archaean gold ores belong to a coherent genetic group of structurally controlled lode-deposits that are characteristically enriched in Au with variable enrichments in Ag, As, W, Sb, Bi, Te, B and Pb, but rarely Cu or Zn, and are surrounded by wallrock alteration haloes enriched in K, LILE and CO2, with variable Na and/or Ca addition. Evidence from the Yilgarn Block of Western Australia, combined with similar evidence from Canada and elsewhere, indicates that such deposits represent a crustal continuum that formed under a variety of crustal regimes over at least a 15 km crustal profile at PT conditions ranging from 180°C at <1 kb to 700°C at 5 kb. Individual deposits, separated by tens to hundreds of kilometres, collectively show transitional variations in structural style of mineralisation, vein textures, and mineralogy of wallrock alteration that relate to the PT conditions of their formation at varying crustal depths. Specific transitions within the total spectrum may be shown also by deposits within gold camps, although nowhere is the entire continuum of deposits recorded from a single gold camp or even greenstone belt. Recognition of the crustal continuum of deposits implicates the existence of giant late-Archaean hydrothermal systems with a deep source for the primary ore fluid. A number of deep fluid and solute reservoirs are possible, including the basal segments of greenstone belts, deep-level intrusive granitoids, mid-to lower-crustal granitoidgneisses, mantle lithosphere, or even subducted oceanic lithosphere, given the probable convergent-margin setting of the host greenstone terranes. Individual stable and radiogenic isotope ratios of fluid and solute components implicate fluid evolution from, or equilibrium with, a number of these reservoirs, stressing the potential complexity of pathways for fluid advection to depositional sites. Lead and strontium isotope ratios of ore-associated minerals provide the most persuasive evidence for fluid advection through deep-level intrusive granitoids or granitoid-gneiss crust, whereas preliminary oxygen isotope data show that mixing of deeply sourced fluid and surface waters only occurred at the highest crustal levels recorded by the lode gold deposits.