A Quantitative Chaperone Interaction Network Reveals the Architecture of Cellular Protein Homeostasis Pathways
Mikko Taipale,George Tucker,Jian Peng,Irina Krykbaeva,Zhen Yuan Lin,Brett Larsen,Hyungwon Choi,Bonnie Berger,Anne-Claude Gingras,Anne-Claude Gingras,Susan Lindquist,Susan Lindquist +11 more
TLDR
It is established that NUDC family cochaperones specifically associate with structurally related but evolutionarily distinct β-propeller folds, providing a framework for deciphering the proteostasis network and its regulation in development and disease and expand the use of chaperones as sensors for drug-target engagement.About:
This article is published in Cell.The article was published on 2014-07-17 and is currently open access. It has received 328 citations till now. The article focuses on the topics: Co-chaperone & Proteostasis.read more
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High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities
Traver Hart,Megha Chandrashekhar,Michael Aregger,Zachary Steinhart,Kevin R. Brown,Graham MacLeod,Monika Mis,Michal Zimmermann,Amélie Fradet-Turcotte,Song Sun,Patricia Mero,Peter B. Dirks,Sachdev S. Sidhu,Frederick P. Roth,Olivia S. Rissland,Daniel Durocher,Daniel Durocher,Stephane Angers,Jason Moffat,Jason Moffat +19 more
TL;DR: It is demonstrated that context-dependent fitness genes accurately recapitulate pathway-specific genetic vulnerabilities induced by known oncogenes and reveal cell-type-specific dependencies for specific receptor tyrosine kinases, even in oncogenic KRAS backgrounds.
Journal ArticleDOI
The BioPlex Network: A Systematic Exploration of the Human Interactome
Edward L. Huttlin,Lily Ting,Raphael J. Bruckner,Fana Gebreab,Melanie P. Gygi,John Szpyt,Stanley Tam,Gabriela Zarraga,Greg Colby,Kurt Baltier,Rui Dong,Virginia Guarani,Laura Pontano Vaites,Alban Ordureau,Ramin Rad,Brian K. Erickson,Martin Wühr,Joel M. Chick,Bo Zhai,Deepak Kolippakkam,Julian Mintseris,Robert A. Obar,Robert A. Obar,Tim Harris,Spyros Artavanis-Tsakonas,Spyros Artavanis-Tsakonas,Mathew E. Sowa,Pietro De Camilli,Joao A. Paulo,J. Wade Harper,Steven P. Gygi +30 more
TL;DR: Using high-throughput affinity-purification mass spectrometry, BioPlex is used to identify interacting partners for 2,594 human proteins in HEK293T cells and reveals associations among thousands of protein domains, suggesting a basis for examining structurally related proteins.
Journal ArticleDOI
A global genetic interaction network maps a wiring diagram of cellular function
Michael Costanzo,Benjamin VanderSluis,Elizabeth N. Koch,Anastasia Baryshnikova,Carles Pons,Guihong Tan,Wen Wang,Matej Usaj,Julia Hanchard,Susan D. Lee,Vicent Pelechano,Erin B. Styles,Maximilian Billmann,Jolanda van Leeuwen,Nydia Van Dyk,Zhen Yuan Lin,Elena Kuzmin,Justin Nelson,Jeff S. Piotrowski,Tharan Srikumar,Sondra Bahr,Yiqun Chen,Raamesh Deshpande,Christoph F. Kurat,Sheena C. Li,Zhijian Li,Mojca Mattiazzi Usaj,Hiroki Okada,Natasha Pascoe,Bryan Joseph San Luis,Sara Sharifpoor,Emira Shuteriqi,Scott W. Simpkins,Jamie Snider,Harsha Garadi Suresh,Yizhao Tan,Hongwei Zhu,Noël Malod-Dognin,Vuk Janjić,Natasa Przulj,Natasa Przulj,Olga G. Troyanskaya,Igor Stagljar,Tian Xia,Tian Xia,Yoshikazu Ohya,Anne-Claude Gingras,Brian Raught,Michael Boutros,Lars M. Steinmetz,Lars M. Steinmetz,Claire Moore,Adam P. Rosebrock,Amy A. Caudy,Chad L. Myers,Brenda J. Andrews,Charles Boone +56 more
TL;DR: A global genetic interaction network highlights the functional organization of a cell and provides a resource for predicting gene and pathway function and how coherent sets of negative or positive genetic interactions connect protein complex and pathways to map a functional wiring diagram of the cell.
Journal ArticleDOI
The HSP90 chaperone machinery
TL;DR: Owing to the importance of HSP90 in the regulation of many cellular proteins, it has become a promising drug target for the treatment of several diseases, which include cancer and diseases associated with protein misfolding.
Journal ArticleDOI
In vivo aspects of protein folding and quality control
TL;DR: A new view of protein folding is emerging, whereby the energy landscapes that proteins navigate during folding in vivo may differ substantially from those observed during refolding in vitro.
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