Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies.
Alina Baum,Benjamin O. Fulton,Elzbieta Wloga,Richard Copin,Kristen E. Pascal,Vincenzo Russo,Stephanie Giordano,Kathryn Lanza,Nicole Negron,Min Ni,Yi Wei,Gurinder S. Atwal,Andrew J. Murphy,Neil Stahl,George D. Yancopoulos,Christos A. Kyratsous +15 more
TLDR
This work investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails.Abstract:
Antibodies targeting the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present a promising approach to combat the coronavirus disease 2019 (COVID-19) pandemic; however, concerns remain that mutations can yield antibody resistance. We investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared after in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization; such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Escape mutants were not generated after treatment with a noncompeting antibody cocktail.read more
Citations
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Architecture and antigenicity of the nipah virus attachment glycoprotein
Zhaoqian Wang,Moushimi Amaya,Amin Addetia,Ha V. Dang,Gabriella Reggiano,Lianying Yan,Andrew C. Hickey,Frank DiMaio,Christopher C. Broder,David Veesler +9 more
TL;DR: A cocktail of two nonoverlapping G-specific antibodies neutralizes NiV and HeV synergistically and limits the emergence of escape mutants, paving the way for implementing multipronged therapeutic strategies against these deadly pathogens.
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Genomic epidemiology of SARS-CoV-2 in Esteio, Rio Grande do Sul, Brazil.
Vinícius Bonetti Franceschi,Gabriel Dickin Caldana,Amanda de Menezes Mayer,Gabriela Bettella Cybis,Carla Andretta Moreira Neves,Patrícia Aline Gröhs Ferrareze,Meriane Demoliner,Paula Rodrigues de Almeida,Juliana Schons Gularte,Alana Witt Hansen,Matheus Nunes Weber,Juliane Deise Fleck,Ricardo Ariel Zimerman,Livia Kmetzsch,Fernando Rosado Spilki,Claudia Elizabeth Thompson,Claudia Elizabeth Thompson +16 more
TL;DR: In this article, the authors track molecular evolution and spread of SARS-CoV-2 in Esteio (Southern Brazil) using phylogenetics and phylodynamics inferences from 21 new genomes in global and regional context Importantly, the case fatality rate (CFR) is slightly higher compared to the Rio Grande do Sul (RS) state (256%) and the entire Brazil (274%)
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An internally validated prediction model for critical COVID-19 infection and intensive care unit admission in symptomatic pregnant women.
Zhiwei Huang,Erkan Kalafat,Smriti Prasad,Pinar Birol,Arzu Bilge Tekin,Atilla Kunt,Carolina Di Fabrizio,Cengiz Alatas,Ebru Celik,Helin Bagci,Julia Binder,Kirsty Le Doare,L A Magee,Memis Ali Mutlu,Murat Yassa,Niyazi Tug,Orhan Sahin,Panagiotis Krokos,Pat O'Brien,Peter von Dadelszen,Pilar Palmrich,George J. Papaioannou,Reyhan Ayaz,Shamez N Ladhani,Sophia Kalantaridou,Veli Mihmanli,Asma Khalil +26 more
TL;DR: In this article, the authors developed a prediction model to quantify the risk of progression to critical COVID-19 and intensive care unit admission in pregnant women with symptomatic infection, and the predictive accuracy was assessed by the area under the receiver operating characteristic curves.
Journal ArticleDOI
A pseudovirus system enables deep mutational scanning of the full SARS-CoV-2 spike
Bernadeta Dadonaite,Katharine H.D. Crawford,Caelan E. Radford,A. G. Farrell,Timothy C. Yu,William W. Hannon,Panpan Zhou,Raiees Andrabi,Dennis R. Burton,Lihong Liu,David D. Ho,Richard A. Neher,Jesse D. Bloom +12 more
TL;DR: In this paper , a deep mutational scanning platform based on non-replicative pseudotyped lentiviruses was proposed to directly quantifies how large numbers of spike mutations impact antibody neutralization and pseudovirus infection.
Posted ContentDOI
Multivalency transforms SARS-CoV-2 antibodies into broad and ultrapotent neutralizers
Edurne Rujas,Edurne Rujas,Iga Kucharska,Yong Zi Tan,Samir Benlekbir,Hong Cui,Tiantian Zhao,Gregory A. Wasney,Patrick Budylowski,Furkan Guvenc,Jocelyn C. Newton,Taylor Sicard,Anthony Semesi,Krithika Muthuraman,Amy Nouanesengsy,Katherine Prieto,Stephanie A. Bueler,Sawsan Youssef,Sindy Liao-Chan,Jacob Glanville,Natasha Christie-Holmes,Samira Mubareka,Samira Mubareka,Scott D. Gray-Owen,John L. Rubinstein,Bebhinn Treanor,Jean-Philippe Julien +26 more
TL;DR: A MULTi-specific, multi-Affinity antiBODY (Multabody) platform contributes a new class of medical countermeasures against COVID-19 and an efficient approach to rapidly deploy potent and broadly-acting therapeutics against infectious diseases of global health importance.
References
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TL;DR: Several monoclonal antibodies that target the S glycoprotein of SARS-CoV-2, which was identified from memory B cells of an individual who was infected with severe acute respiratory syndrome coronavirus (SARS- coV) in 2003, and one antibody (named S309) potently neutralization, which may limit the emergence of neutralization-escape mutants.
Journal ArticleDOI
Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells.
Y Cao,Bin Su,Xianghua Guo,Wenjie Sun,Yong-Qiang Deng,Linlin Bao,Qinyu Zhu,Xu Zhang,Yinghui Zheng,Chenyang Geng,Xiaoran Chai,Runsheng He,Xiaofeng Li,Qi Lv,Hua Zhu,Wei Deng,Yanfeng Xu,Yanjun Wang,Luxin Qiao,Yafang Tan,Liyang Song,Guopeng Wang,Xiao-Xia Du,Ning Gao,Jiangning Liu,Junyu Xiao,Xiao-Dong Su,Zongmin Du,Yingmei Feng,Chuan Qin,Cheng-Feng Qin,Ronghua Jin,X. Sunney Xie +32 more
TL;DR: It is shown that human neutralizing antibodies could be efficiently discovered by high-throughput single B-cell sequencing in response to pandemic infectious diseases.
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