Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies.
Alina Baum,Benjamin O. Fulton,Elzbieta Wloga,Richard Copin,Kristen E. Pascal,Vincenzo Russo,Stephanie Giordano,Kathryn Lanza,Nicole Negron,Min Ni,Yi Wei,Gurinder S. Atwal,Andrew J. Murphy,Neil Stahl,George D. Yancopoulos,Christos A. Kyratsous +15 more
TLDR
This work investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails.Abstract:
Antibodies targeting the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present a promising approach to combat the coronavirus disease 2019 (COVID-19) pandemic; however, concerns remain that mutations can yield antibody resistance. We investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared after in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization; such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Escape mutants were not generated after treatment with a noncompeting antibody cocktail.read more
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SARS-CoV-2 variants – Evolution, spike protein, and vaccines
TL;DR: In this article , the authors describe the evolution of SARS-CoV-2, summarize current knowledge of epidemiological and clinical features of the variants, and discuss the response strategies in terms of vaccines to reduce the burden of COVID-19.
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High resolution profiling of pathways of escape for SARS-CoV-2 spike-binding antibodies
Meghan E. Garrett,Jared Galloway,Helen Y. Chu,Hannah L. Itell,Caitlin I. Stoddard,Caitlin R Wolf,Jennifer Logue,Dylan McDonald,Frederick A. Matsen,Julie Overbaugh +9 more
TL;DR: A granular view of potential antibody escape pathways is provided and it is suggested there will be individual variation in antibody-mediated virus evolution.
Journal ArticleDOI
Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate Immunity.
Ulfert Rand,Sascha Young Kupke,Hanna Shkarlet,Marc D. Hein,Tatjana Hirsch,Pavel Marichal-Gallardo,Luka Cicin-Sain,Udo Reichl,Udo Reichl,Dunja Bruder +9 more
TL;DR: In this article, the applicability of IAV DIPs as an antiviral for the treatment of COVID-19, was investigated by conducting in vitro co-infection experiments with cell culture-derived IAV defective interfering particles and the IFN-sensitive SARS-CoV-2 in human lung cells.
Posted ContentDOI
Chronic SARS-CoV-2 infection and viral evolution in a hypogammaglobulinaemic individual
Maia Kavanagh Williamson,Fergus Hamilton,Fergus Hamilton,Stephanie Hutchings,Hannah M Pymont,Mark Hackett,David T Arnold,Nick A Maskell,Alasdair P. MacGowan,Mahableshwar Albur,Megan Jenkins,Izak Heys,Francesca Knapper,Mustafa Elsayed,Rachel Milligan,Peter Muir,Barry Vipond,David A. Matthews,Ed Moran,Andrew D. Davidson +19 more
TL;DR: In this article, the authors reported on an immunocompromised individual who was persistently infected with SARS-CoV-2 for over 290 days, the longest persistent infection recorded in the literature to date.
References
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Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody.
Dora Pinto,Young-Jun Park,Martina Beltramello,Alexandra C. Walls,M. Alejandra Tortorici,M. Alejandra Tortorici,Siro Bianchi,Stefano Jaconi,Katja Culap,Fabrizia Zatta,Anna De Marco,Alessia Peter,Barbara Guarino,Roberto Spreafico,Elisabetta Cameroni,James Brett Case,Rita E. Chen,Colin Havenar-Daughton,Gyorgy Snell,Amalio Telenti,Herbert W. Virgin,Antonio Lanzavecchia,Michael S. Diamond,Katja Fink,David Veesler,Davide Corti +25 more
TL;DR: Several monoclonal antibodies that target the S glycoprotein of SARS-CoV-2, which was identified from memory B cells of an individual who was infected with severe acute respiratory syndrome coronavirus (SARS- coV) in 2003, and one antibody (named S309) potently neutralization, which may limit the emergence of neutralization-escape mutants.
Journal ArticleDOI
Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells.
Y Cao,Bin Su,Xianghua Guo,Wenjie Sun,Yong-Qiang Deng,Linlin Bao,Qinyu Zhu,Xu Zhang,Yinghui Zheng,Chenyang Geng,Xiaoran Chai,Runsheng He,Xiaofeng Li,Qi Lv,Hua Zhu,Wei Deng,Yanfeng Xu,Yanjun Wang,Luxin Qiao,Yafang Tan,Liyang Song,Guopeng Wang,Xiao-Xia Du,Ning Gao,Jiangning Liu,Junyu Xiao,Xiao-Dong Su,Zongmin Du,Yingmei Feng,Chuan Qin,Cheng-Feng Qin,Ronghua Jin,X. Sunney Xie +32 more
TL;DR: It is shown that human neutralizing antibodies could be efficiently discovered by high-throughput single B-cell sequencing in response to pandemic infectious diseases.
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