Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies.
Alina Baum,Benjamin O. Fulton,Elzbieta Wloga,Richard Copin,Kristen E. Pascal,Vincenzo Russo,Stephanie Giordano,Kathryn Lanza,Nicole Negron,Min Ni,Yi Wei,Gurinder S. Atwal,Andrew J. Murphy,Neil Stahl,George D. Yancopoulos,Christos A. Kyratsous +15 more
TLDR
This work investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails.Abstract:
Antibodies targeting the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present a promising approach to combat the coronavirus disease 2019 (COVID-19) pandemic; however, concerns remain that mutations can yield antibody resistance. We investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared after in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization; such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Escape mutants were not generated after treatment with a noncompeting antibody cocktail.read more
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A critical overview of current progress for COVID-19: development of vaccines, antiviral drugs, and therapeutic antibodies
Monika Kumari,Ruei-Min Lu,Mu-Chun Li,Jhih-Liang Huang,Fu-Fei Hsu,Shih-Han Ko,Feng-Yi Ke,Shih-Chieh Su,Kang-Hao Liang,Joyce Pei-Yi Yuan,H.L. Chiang,Cheng-Pu Sun,I-Jung Lee,Wen-Shan Li,Hsing Pang Hsieh,Mi-Hua Tao,Han-Chung Wu +16 more
TL;DR: In this paper , the authors provide a framework for understanding the ongoing development efforts for different types of vaccines and therapeutics, including small molecule and antibody drugs, and summarize the clinical trials supporting the development and distribution of vaccines, small molecule drugs and therapeutic antibodies with broad spectrum activity against SARS-CoV-2 strains.
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COVA1-18 neutralizing antibody protects against SARS-CoV-2 in three preclinical models
Pauline Maisonnasse,Yoann Aldon,Aurélien Marc,Romain Marlin,Nathalie Dereuddre-Bosquet,Natalia Kuzmina,Alec W. Freyn,Jonne L. Snitselaar,Antonio Gonçalves,Tom G. Caniels,Judith A. Burger,Meliawati Poniman,Ilja Bontjer,Virginie Chesnais,Ségolène Diry,Anton Iershov,Adam J. Ronk,Sonia Jangra,Raveen Rathnasinghe,Philip J. M. Brouwer,Tom P. L. Bijl,Jelle van Schooten,Mitch Brinkkemper,Hejun Liu,Meng Yuan,Chad E. Mire,Mariëlle J. van Breemen,Vanessa Contreras,Thibaut Naninck,Julien Lemaitre,Nidhal Kahlaoui,Francis Relouzat,Catherine Chapon,Raphael Ho Tsong Fang,Charlene McDanal,Mary Osei-Twum,Natalie St-Amant,Luc Gagnon,David C. Montefiori,Ian A. Wilson,Eric Ginoux,Godelieve J. de Bree,Adolfo García-Sastre,Michael Schotsaert,Lynda Coughlan,Lynda Coughlan,Alexander Bukreyev,Sylvie van der Werf,Sylvie van der Werf,Jeremie Guedj,Rogier W. Sanders,Rogier W. Sanders,Marit J. van Gils,Roger Le Grand +53 more
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An early warning system for emerging SARS-CoV-2 variants
Lorenzo Subissi,Anne von Gottberg,Lipi Thukral,Nathalie Worp,Bas B. Oude Munnink,Surabhi Rathore,Laith J. Abu-Raddad,Ximena Aguilera,E. A. Alm,Brett N. Archer,Homa Attar Cohen,Amal Barakat,Wendy S. Barclay,Jinal N. Bhiman,Leon Caly,Meera Chand,Mark Chen,Ann Cullinane,Tulio de Oliveira,Christian Drosten,Julian Druce,P Effler,Ihab El Masry,Adama Faye,Simani Gaseitsiwe,Elodie Ghedin,Rebecca L. Grant,Bart L. Haagmans,Belinda L. Herring,Shilpa S Iyer,Zyleen Kassamali,Manish Kakkar,Rebecca Kondor,Juliana Alves Leite,Yee Sin Leo,Gabriel M. Leung,Marco Marklewitz,Sikhulile Moyo,Jairo Mendez-Rico,Nada M. Melhem,Vincent J. Munster,Karen Martun Nahapetyan,Djin-Ye Oh,Boris I. Pavlin,Thomas P. Peacock,Malik Peiris,Zhibin Peng,Leo L. M. Poon,Andrew Rambaut,Jilian A. Sacks,Yinzhong Shen,Marilda M. Siqueira,Sofonias K. Tessema,Erik M. Volz,Volker Thiel,S. Van Der Werf,Sylvie Briand,Mark D. Perkins,Maria D. Van Kerkhove,Marion Koopmans,Anurag Agrawal +60 more
TL;DR: In this paper , global sequencing and surveillance capacity for SARS-CoV-2 must be strengthened and combined with multidisciplinary studies of infectivity, virulence and immune escape, in order to track the unpredictable evolution of the ongoing COVID-19 pandemic.
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SARS-CoV-2 Entry Related Viral and Host Genetic Variations: Implications on COVID-19 Severity, Immune Escape, and Infectivity.
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TL;DR: In this paper, a review summarizes the latest research to describe the impacts of genetic variation in the spike glycoprotein and critical host proteins and aims to provide better insights for understanding transmission and pathogenesis and more broadly for developing vaccine/antiviral drugs and precision medicine strategies, especially for high risk populations with genetic risk variants.
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Spread of Mink SARS-CoV-2 Variants in Humans: A Model of Sarbecovirus Interspecies Evolution.
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References
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TL;DR: The crystal structure of the C-terminal domain of SARS-CoV-2 (SARS- coV- 2-CTD) spike (S) protein in complex with human ACE2 (hACE2) is presented, which reveals a hACE2-binding mode similar overall to that observed for SARS -CoV.
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Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody.
Dora Pinto,Young-Jun Park,Martina Beltramello,Alexandra C. Walls,M. Alejandra Tortorici,M. Alejandra Tortorici,Siro Bianchi,Stefano Jaconi,Katja Culap,Fabrizia Zatta,Anna De Marco,Alessia Peter,Barbara Guarino,Roberto Spreafico,Elisabetta Cameroni,James Brett Case,Rita E. Chen,Colin Havenar-Daughton,Gyorgy Snell,Amalio Telenti,Herbert W. Virgin,Antonio Lanzavecchia,Michael S. Diamond,Katja Fink,David Veesler,Davide Corti +25 more
TL;DR: Several monoclonal antibodies that target the S glycoprotein of SARS-CoV-2, which was identified from memory B cells of an individual who was infected with severe acute respiratory syndrome coronavirus (SARS- coV) in 2003, and one antibody (named S309) potently neutralization, which may limit the emergence of neutralization-escape mutants.
Journal ArticleDOI
Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells.
Y Cao,Bin Su,Xianghua Guo,Wenjie Sun,Yong-Qiang Deng,Linlin Bao,Qinyu Zhu,Xu Zhang,Yinghui Zheng,Chenyang Geng,Xiaoran Chai,Runsheng He,Xiaofeng Li,Qi Lv,Hua Zhu,Wei Deng,Yanfeng Xu,Yanjun Wang,Luxin Qiao,Yafang Tan,Liyang Song,Guopeng Wang,Xiao-Xia Du,Ning Gao,Jiangning Liu,Junyu Xiao,Xiao-Dong Su,Zongmin Du,Yingmei Feng,Chuan Qin,Cheng-Feng Qin,Ronghua Jin,X. Sunney Xie +32 more
TL;DR: It is shown that human neutralizing antibodies could be efficiently discovered by high-throughput single B-cell sequencing in response to pandemic infectious diseases.
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