Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor.
Wulan Deng,Wulan Deng,Jongjoo Lee,Hongxin Wang,Jeffrey C. Miller,Andreas Reik,Philip D. Gregory,Ann Dean,Gerd A. Blobel,Gerd A. Blobel +9 more
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TLDR
These findings establish Ldb1 as a critical effector of GATA1-mediated loop formation and indicate that chromatin looping causally underlies gene regulation.About:
This article is published in Cell.The article was published on 2012-06-08 and is currently open access. It has received 647 citations till now. The article focuses on the topics: Locus control region & Chromatin Loop.read more
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Cas9 as a versatile tool for engineering biology
TL;DR: The Cas9 targeting methodology is described, current and prospective engineering advances are detail and potential applications ranging from basic science to the clinic are suggested.
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Exploring the three-dimensional organization of genomes: interpreting chromatin interaction data
TL;DR: Several types of statistical and computational approaches that have recently been developed to analyse chromatin interaction data are described.
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Organization and function of the 3D genome.
TL;DR: The insights into chromatin architecture that have been gained through recent technological developments in quantitative biology, genomics and cell and molecular biology approaches are discussed and how these new concepts have been used to address important biological questions in development and disease are explained.
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The Mediator complex: a central integrator of transcription
TL;DR: Although Mediator exists in all eukaryotes, a variety of Mediator functions seem to be specific to metazoans, which is indicative of more diverse regulatory requirements.
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Genome architecture: domain organization of interphase chromosomes
TL;DR: Together with microscopy and computational modeling, the results begin to yield a more coherent picture that integrates linear and three-dimensional views of chromosome organization in relation to gene regulation and other nuclear functions.
References
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Capturing Chromosome Conformation
TL;DR: Using the yeast Saccharomyces cerevisiae, this work could confirm known qualitative features of chromosome organization within the nucleus and dynamic changes in that organization during meiosis and found that chromatin is highly flexible throughout.
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Looping and interaction between hypersensitive sites in the active β-globin locus
TL;DR: It is proposed that clustering of regulatory elements is key to creating and maintaining active chromatin domains and regulating transcription.
Journal ArticleDOI
Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1
Larysa Pevny,M. Celeste Simon,Elizabeth J. Robertson,William H. Klein,Shih-Feng Tsai,Vivette D. D'Agati,Stuart H. Orkin,Frank Costantini +7 more
TL;DR: The disruption of the X-linked GATA-1 gene by homologous recombination in a male (XY) murine embryonic stem cell line and testing the Gata-1-deficient cells for their ability to contribute to different tissues in chimaeric mice demonstrates that GATA, the zinc-finger transcription factor, is required for the normal differentiation of erythroid cells, and that other GATAS cannot compensate for its absence.
Journal ArticleDOI
Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells
Shih-Feng Tsai,David I. K. Martin,David I. K. Martin,Leonard I. Zon,Leonard I. Zon,Alan D. D'Andrea,Alan D. D'Andrea,Gordon G. Wong,Stuart H. Orkin,Stuart H. Orkin +9 more
TL;DR: Genes expressed in erythroid cells contain binding sites for a cell-specific factor believed to be an important regulator for this haematopoietic lineage, and complementary DNA encoding the murine protein is identified using high-level transient expression in mammalian cells.
Journal ArticleDOI
Long-range chromatin regulatory interactions in vivo
TL;DR: The development of a widely applicable in situ technique to tag and recover chromatin in the immediate vicinity of an actively transcribed gene and provide the first direct evidence of long-range enhancer communication is reported.
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