Detection of Staphylococcal Cassette Chromosome mec Type XI Carrying Highly Divergent mecA, mecI, mecR1, blaZ, and ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-Resistant Staphylococcus aureus
Anna C. Shore,E.C. Deasy,Peter Slickers,Gráinne I. Brennan,Brian O'Connell,Stefan Monecke,Ralf Ehricht,David C. Coleman +7 more
TLDR
Two clonal complex 130 methicillin-resistant Staphylococcus aureus isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening, suggesting they may have originated in another taxon.Abstract:
Methicillin resistance in staphylococci is mediated by penicillin binding protein 2a (PBP 2a), encoded by mecA on mobile staphylococcal cassette chromosome mec (SCCmec) elements. In this study, two clonal complex 130 (CC130) methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening. The isolates were identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay. Whole-genome sequencing of one isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. The open reading frames (ORFs) identified within SCCmec XI of M10/0061 exhibited 21 to 93% amino acid identity to ORFs in GenBank. A third DR was identified ca. 3 kb downstream of SCCmec XI, indicating the presence of a possible SCC remnant. SCCmec XI was also identified in the second CC130 MRSA isolate by PCR and sequencing. The CC130 MRSA isolates may be of animal origin as previously reported CC130 S. aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon.read more
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Infecções por Staphylococcus aureus: mudança do perfil epidemiológico no Hospital Universitário Pedro Ernesto Staphiloccocus aureus infections: change in epidemiology at Pedro Ernesto University Hospital
Dissertation
Comparative molecular analysis of Staphylococcus aureus from intensive livestock farming with emphasis on LA-MRSA of poultry origin
TL;DR: Results showed that the current dissemination of LA-MRSA also included chickens and turkeys, and the available tools to track the spread of MRSA were considered and the current knowledge about these organisms from food producing animals and food with respect to the role ofMRSA to act as a food-borne pathogen were summarized.
DetectionDetection Assay Adapted for mecC Methicillin-Resistant Staphylococcus Evaluation of a Modular Multiplex-PCR
TL;DR: The assay’senzyme-linked immunosorbent assay (ELISA) approach, using aperoxidase conjugate that binds specifically on the labeled region of the hybridization product, was used as detection procedure.
Journal ArticleDOI
Presence of antibiotic-resistant bacteria in pigs for consumption - one health challenge - Literature review
Isabela Carvalho dos Santos,Lidiane Nunes Barbosa,Lisiane de Almeida Martins,Laisa Marina Rosa Rey,Kawany Gabrieli Zanetti Fazoli,Lucas Lima da Silva,Larissa Rafaela de Paula Ferreira,Ulisses de Pádua Pereira,Daniela Dib Gonçalves +8 more
TL;DR: In this article , a review aimed to highlight the importance of bacterial resistance within breeding stock, its possible origins, the importance Staphylococcus spp. within this topic, and its evolution in swine farming over the years.
Journal ArticleDOI
Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil
Regina Célia Ruiz,Valcilaine Teixeira Barbosa,Teresa de Lisieux Guedes Ferreira Lôbo,Rafael Aleixo dos Santos Silva,Rodrigo José Nunes Calumby,Suzanne Pinheiro Vieira,Diogo Gomes Brandão,Felipe Mendes Zorzi,Rossana Teotônio de Farias Moreira,Valter Alvino,Ticiano Gomes do Nascimento,Luciano Aparecido Meireles Grilo,Camila Braga Dornelas,Elsa Masae Mamizuka,Lara Mendes de Almeida +14 more
TL;DR: SCCmec type variants differing from those so far acknowledged by IWG-SCC were found, indicating new rearrangements in the genetic context of mecA in these canine MRSIG isolates.
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