Detection of Staphylococcal Cassette Chromosome mec Type XI Carrying Highly Divergent mecA, mecI, mecR1, blaZ, and ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-Resistant Staphylococcus aureus
Anna C. Shore,E.C. Deasy,Peter Slickers,Gráinne I. Brennan,Brian O'Connell,Stefan Monecke,Ralf Ehricht,David C. Coleman +7 more
TLDR
Two clonal complex 130 methicillin-resistant Staphylococcus aureus isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening, suggesting they may have originated in another taxon.Abstract:
Methicillin resistance in staphylococci is mediated by penicillin binding protein 2a (PBP 2a), encoded by mecA on mobile staphylococcal cassette chromosome mec (SCCmec) elements. In this study, two clonal complex 130 (CC130) methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening. The isolates were identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay. Whole-genome sequencing of one isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. The open reading frames (ORFs) identified within SCCmec XI of M10/0061 exhibited 21 to 93% amino acid identity to ORFs in GenBank. A third DR was identified ca. 3 kb downstream of SCCmec XI, indicating the presence of a possible SCC remnant. SCCmec XI was also identified in the second CC130 MRSA isolate by PCR and sequencing. The CC130 MRSA isolates may be of animal origin as previously reported CC130 S. aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon.read more
Citations
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Searching Biomarkers in the Sequenced Genomes of Staphylococcus for their Rapid Identification
TL;DR: The genomes of 31 strains of Staphylococcus representing 7 species were searched for the presence of common genes, and by employing additional RE and genes unique to a particular strain, it was possible to identify the rest 6 Staphyllococcus aureus strains.
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A novel multidrug-resistant PVL-negative CC1-MRSA-IV clone emerging in Ireland and Germany likely originated in South-Eastern Europe.
Megan R. Earls,Anna C. Shore,Gráinne I. Brennan,Alexandra Simbeck,Wulf Schneider-Brachert,Teodora Vremerǎ,Olivia Dorneanu,Peter Slickers,Ralf Ehricht,Stefan Monecke,Stefan Monecke,David C. Coleman +11 more
TL;DR: The existence of a European PVL-negative CC1-MRSA-IV clone is demonstrated that may have originated in South-Eastern Europe, before spreading to both Ireland and Germany, and which is distinctly different from the well-defined Western Australia MRSA-1 clone.
Book ChapterDOI
Molecular Epidemiology of Staphylococcus aureus Lineages in the Animal–Human Interface
TL;DR: This review will analyze the characteristics of these lineages in the animal–human interface, special attention will be given to lineage CC398 for which the evolution and potential public health concerns will be analyzed, and lineage CC130 specially distributed not only in wild animals and livestock but also emerging in humans.
Methicillin- and vancomycin-resistant Staphylococcus aureus in health care workers and medical devices Staphylococcus aureus resistentes a meticilina (MRSA) e vancomicina (VRSA) em profissionais da saúde e artigos médicos
Angela Breves,Catia Aparecida,Catia Chaia de Miranda,Claudia Flores,Ivano de Filippis,Maysa M. Clementino +5 more
TL;DR: In this paper, the authors investigated the colonization and the antimicrobial susceptibility profile of Staphylococcus spp. on surfaces of medical articles and in professionals from two basic health units in the city of Rio de Janeiro.
Journal ArticleDOI
Detection of a mecC-positive Staphylococcus saprophyticus from bovine mastitis in Argentina.
TL;DR: This study reports for the first time the mecC gene from a CNS in bovine mastitis in South America, seen as a potential public health threat with respect to antimicrobial resistance and the development of multiple resistance.
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