Detection of Staphylococcal Cassette Chromosome mec Type XI Carrying Highly Divergent mecA, mecI, mecR1, blaZ, and ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-Resistant Staphylococcus aureus
Anna C. Shore,E.C. Deasy,Peter Slickers,Gráinne I. Brennan,Brian O'Connell,Stefan Monecke,Ralf Ehricht,David C. Coleman +7 more
TLDR
Two clonal complex 130 methicillin-resistant Staphylococcus aureus isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening, suggesting they may have originated in another taxon.Abstract:
Methicillin resistance in staphylococci is mediated by penicillin binding protein 2a (PBP 2a), encoded by mecA on mobile staphylococcal cassette chromosome mec (SCCmec) elements. In this study, two clonal complex 130 (CC130) methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening. The isolates were identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay. Whole-genome sequencing of one isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. The open reading frames (ORFs) identified within SCCmec XI of M10/0061 exhibited 21 to 93% amino acid identity to ORFs in GenBank. A third DR was identified ca. 3 kb downstream of SCCmec XI, indicating the presence of a possible SCC remnant. SCCmec XI was also identified in the second CC130 MRSA isolate by PCR and sequencing. The CC130 MRSA isolates may be of animal origin as previously reported CC130 S. aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon.read more
Citations
More filters
Journal ArticleDOI
Molecular mechanisms of antibiotic resistance.
TL;DR: Recent advances in understanding of the mechanisms by which bacteria are either intrinsically resistant or acquire resistance to antibiotics are reviewed, including the prevention of access to drug targets, changes in the structure and protection of antibiotic targets and the direct modification or inactivation of antibiotics.
Journal ArticleDOI
Coagulase-Negative Staphylococci
TL;DR: Therapeutically, CoNS are challenging due to the large proportion of methicillin-resistant strains and increasing numbers of isolates with less susceptibility to glycopeptides, and host susceptibility is much more important.
Journal ArticleDOI
Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology
Sahreena Lakhundi,Kunyan Zhang +1 more
TL;DR: The origin of MRSA is described, with emphasis on the diverse nature of staphylococcal cassette chromosome mec (SCCmec).
Journal ArticleDOI
A review of the influence of treatment strategies on antibiotic resistant bacteria and antibiotic resistance genes
TL;DR: This review begins with an introduction to the occurrence of ARB and ARG in different environmental systems such as natural environments and drinking water resources, and the mechanism of the effects of different disinfection processes in water and wastewater.
Journal ArticleDOI
The emergence of mecC methicillin-resistant Staphylococcus aureus.
TL;DR: A novel mecA homologue, mecC, confers methamphetamineicillin resistance to methicillin-resistant Staphylococcus aureus (MRSA) and may pose a zoonotic risk to humans.
References
More filters
Journal ArticleDOI
Community-associated MRSA SCCmec type IVd in Irish equids.
Yasunori Maeda,B. Cherie Millar,Anne Loughrey,Colin E. Goldsmith,Paul J. Rooney,John E. Moore,J. R. Rao,Thomas Buckley,Claire L. Egan,James S. G. Dooley,Colm J. Lowery,Motoo Matsuda +11 more
TL;DR: This report describes the presence of PVL toxin-negative MRSA organisms, belonging to the SCCmec IVd subclass in Irish equids, suggesting a community origin for these organisms, as opposed to a health care origin.
Related Papers (5)
Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study.
Laura García-Álvarez,Matthew T. G. Holden,Heather Lindsay,Cerian R. Webb,Derek F. J. Brown,Martin D. Curran,Enid Walpole,Karen Brooks,Derek Pickard,Christopher Teale,Julian Parkhill,Stephen D. Bentley,Giles Edwards,E Kirsty Girvan,Angela M. Kearns,Bruno Pichon,Robert Hill,Anders Rhod Larsen,Robert Skov,Sharon J. Peacock,Duncan J. Maskell,Mark A. Holmes +21 more
Classification of staphylococcal cassette chromosome mec (SCCmec) : guidelines for reporting novel SCCmec elements.
Teruyo Ito,Keiichi Hiramatsu,D. Oliviera,H. de Lencastre,Kunyan Zhang,Henrik Westh,Frances G. O'Brien,Philip M. Giffard,David C. Coleman,Fred C. Tenover,Susan Boyle-Vavra,Robert Skov,Mark C. Enright,Barry N. Kreiswirth,Kwan Soo Ko,Hajo Grundmann,Frédéric Laurent,Johanna U. Ericson Sollid,Angela M. Kearns,Angela M. Kearns,Richard V. Goering,Joseph F. John,Robert S. Daum,Bo Söderquist +23 more
A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus
Stefan Monecke,Geoffrey W. Coombs,Anna C. Shore,David C. Coleman,Patrick Eberechi Akpaka,Michael Borg,Henry Chow,Margaret Ip,Lutz Jatzwauk,Daniel Jonas,Kristina Kadlec,Angela M. Kearns,Frédéric Laurent,Frances G. O'Brien,Julie C. Pearson,Antje Ruppelt,Stefan Schwarz,E. Scicluna,Peter Slickers,Hui-Leen Tan,Stefan Weber,Ralf Ehricht +21 more