Detection of Staphylococcal Cassette Chromosome mec Type XI Carrying Highly Divergent mecA, mecI, mecR1, blaZ, and ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-Resistant Staphylococcus aureus
Anna C. Shore,E.C. Deasy,Peter Slickers,Gráinne I. Brennan,Brian O'Connell,Stefan Monecke,Ralf Ehricht,David C. Coleman +7 more
TLDR
Two clonal complex 130 methicillin-resistant Staphylococcus aureus isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening, suggesting they may have originated in another taxon.Abstract:
Methicillin resistance in staphylococci is mediated by penicillin binding protein 2a (PBP 2a), encoded by mecA on mobile staphylococcal cassette chromosome mec (SCCmec) elements. In this study, two clonal complex 130 (CC130) methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening. The isolates were identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay. Whole-genome sequencing of one isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. The open reading frames (ORFs) identified within SCCmec XI of M10/0061 exhibited 21 to 93% amino acid identity to ORFs in GenBank. A third DR was identified ca. 3 kb downstream of SCCmec XI, indicating the presence of a possible SCC remnant. SCCmec XI was also identified in the second CC130 MRSA isolate by PCR and sequencing. The CC130 MRSA isolates may be of animal origin as previously reported CC130 S. aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon.read more
Citations
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Epidemiology of methicillin-resistant Staphylococcus aureus carrying the novel mecC gene in Denmark corroborates a zoonotic reservoir with transmission to humans
Andreas Petersen,Mikael Ruge Stegger,Ole Heltberg,Jens Jørgen Christensen,A Zeuthen,Lisbet Krause Knudsen,Tinna Ravnholt Urth,M. Sørum,L Schouls,Jesper Larsen,Robert Skov,Anders Rhod Larsen +11 more
TL;DR: Observations indicate that mecC-carrying MRSA can be exchanged between humans and ruminants, being older than other CA-MRSA patients.
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High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.
Joana Rolo,Maria Miragaia,Agata Turlej-Rogacka,Joanna Empel,Ons Bouchami,Nuno A. Faria,Ana Tavares,Waleria Hryniewicz,Ad C. Fluit,Hermínia de Lencastre,Hermínia de Lencastre +10 more
TL;DR: The results imply that CA-MRSA origin, epidemiology and population structure in Europe is very dissimilar from that of USA.
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Guidelines for Reporting Novel mecA Gene Homologues
Teruyo Ito,Keiichi Hiramatsu,Alexander Tomasz,Hermínia de Lencastre,Vincent Perreten,Matthew T. G. Holden,David C. Coleman,Richard V. Goering,Philip M. Giffard,Robert Skov,Kunyan Zhang,Henrik Westh,Frances G. O'Brien,Fred C. Tenover,Duarte C. Oliveira,Susan Boyle-Vavra,Frédéric Laurent,Angela M. Kearns,Barry N. Kreiswirth,Kwan Soo Ko,Hajo Grundmann,Johanna U. Ericson Sollid,Joseph F. John,Robert S. Daum,Bo Söderquist,Girbe Buist +25 more
TL;DR: This study highlights the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds of staphylococci.
Journal ArticleDOI
The newly described mecA homologue, mecALGA251, is present in methicillin-resistant Staphylococcus aureus isolates from a diverse range of host species
Gavin K. Paterson,Anders Rhod Larsen,A Robb,GE Edwards,T. W. Pennycott,G. Foster,Dorien Mot,Katleen Hermans,Kristof Baert,Sharon J. Peacock,Sharon J. Peacock,Julian Parkhill,Ruth N. Zadoks,Mark A. Holmes +13 more
TL;DR: The discovery of MRSA isolates possessing mecA(LGA251) from a diverse range of host species, including different taxonomic classes, has important implications for the diagnosis ofMRSA in these species and the understanding of the epidemiology of this novel mec aureus homologue.
Journal ArticleDOI
Bacteria from Animals as a Pool of Antimicrobial Resistance Genes.
Maria Angeles Argudín,Ariane Deplano,Alaeddine Meghraoui,Magali Dodémont,Amélie Heinrichs,Olivier Denis,Claire Nonhoff,Sandrine Roisin +7 more
TL;DR: The role of bacteria from animals as a pool of antimicrobial resistance genes for human bacteria is explored and the current knowledge of the use of antimicrobials in animal health is summarized.
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