Detection of Staphylococcal Cassette Chromosome mec Type XI Carrying Highly Divergent mecA, mecI, mecR1, blaZ, and ccr Genes in Human Clinical Isolates of Clonal Complex 130 Methicillin-Resistant Staphylococcus aureus
Anna C. Shore,E.C. Deasy,Peter Slickers,Gráinne I. Brennan,Brian O'Connell,Stefan Monecke,Ralf Ehricht,David C. Coleman +7 more
TLDR
Two clonal complex 130 methicillin-resistant Staphylococcus aureus isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening, suggesting they may have originated in another taxon.Abstract:
Methicillin resistance in staphylococci is mediated by penicillin binding protein 2a (PBP 2a), encoded by mecA on mobile staphylococcal cassette chromosome mec (SCCmec) elements. In this study, two clonal complex 130 (CC130) methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients in Irish hospitals were identified that were phenotypically PBP 2a positive but lacked mecA by conventional PCR and by DNA microarray screening. The isolates were identified as methicillin-susceptible S. aureus using the GeneXpert real-time PCR assay. Whole-genome sequencing of one isolate (M10/0061) revealed a 30-kb SCCmec element encoding a class E mec complex with highly divergent blaZ-mecA-mecR1-mecI, a type 8 cassette chromosome recombinase (ccr) complex consisting of ccrA1-ccrB3, an arsenic resistance operon, and flanking direct repeats (DRs). The SCCmec element was almost identical to that of SCCmec type XI (SCCmec XI) identified by the Sanger Institute in sequence type 425 bovine MRSA strain LGA251 listed on the website of the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements. The open reading frames (ORFs) identified within SCCmec XI of M10/0061 exhibited 21 to 93% amino acid identity to ORFs in GenBank. A third DR was identified ca. 3 kb downstream of SCCmec XI, indicating the presence of a possible SCC remnant. SCCmec XI was also identified in the second CC130 MRSA isolate by PCR and sequencing. The CC130 MRSA isolates may be of animal origin as previously reported CC130 S. aureus strains were predominantly from bovine sources. The highly divergent nature of SCCmec XI relative to other SCCmec elements indicates that it may have originated in another taxon.read more
Citations
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Elementos de interés clínico en la microbiología molecular de Staphylococcus aureus
TL;DR: In this article, an actualización sobre Staphylococcus aureus, resistencia a la meticilina, genes, epidemiologia molecular, virulencia and combinación entre them is presented.
Dissertation
The evolution and transmission of HA-MRSA ST239 through hospitals in Turkey and intercontinental spread
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TL;DR: Acevedo et al. as discussed by the authors used a multiplex PCR for simultaneous detection of mecA and pvl genes in Staphylococcus spp. using 41 isolates of S. aureus.
Posted ContentDOI
An epidemic CC1-MRSA-IV clone yields false negative test results in molecular MRSA identification assays: a note of caution
Stefan Monecke,Stefan Monecke,Elisabeth König,Megan R. Earls,Eva Leitner,Elke Müller,Gabriel E. Wagner,David M. Poitz,Lutz Jatzwauk,Teodora Vremerǎ,Olivia Dorneanu,Alexandra Simbeck,Andreas Ambrosch,Ines Zollner-Schwetz,Robert Krause,Werner Ruppitsch,Wulf Schneider-Brachert,David C. Coleman,Ivo Steinmetz,Ralf Ehricht,Ralf Ehricht +20 more
TL;DR: Investigating why a clinical MRSA isolate yielded false negative test results with widely used, commercial orfX/SCCmec junction site PCR tests found that isolates of the epidemic European CC1-MRSA-IV clone, which likely originated in South-Eastern Europe and subsequently spread to Western Europe, generally exhibit this behaviour.
Book ChapterDOI
Molecular Detection of Staphylococcus aureus Colonization and Infection
TL;DR: Global efforts to reduce MRSA infection rates appear to have had some impact in both Europe and the United States, but improvement has not been uniform, with selected academic medical centers not realizing a reduction in disease and at least one large state reporting an increase in overall MRSA infections.
References
More filters
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Performance standards for antimicrobial susceptibility testing
TL;DR: The supplemental information presented in this document is intended for use with the antimicrobial susceptibility testing procedures published in the following Clinical and Laboratory Standards Institute (CLSI)–approved standards.
Journal ArticleDOI
EMBOSS: The European Molecular Biology Open Software Suite
TL;DR: The European Molecular Biology Open Software Suite is a mature package of software tools developed for the molecular biology community that includes a comprehensive set of applications for molecular sequence analysis and other tasks and integrates popular third-party software packages under a consistent interface.
Journal ArticleDOI
Prodigal: prokaryotic gene recognition and translation initiation site identification
Doug Hyatt,Doug Hyatt,Gwo Liang Chen,Philip F. LoCascio,Miriam Land,Frank W. Larimer,Frank W. Larimer,Loren Hauser +7 more
TL;DR: This work developed a new gene prediction algorithm called Prodigal (PROkaryotic DYnamic programming Gene-finding ALgorithm), which achieved good results compared to existing methods, and it is believed it will be a valuable asset to automated microbial annotation pipelines.
Journal ArticleDOI
Artemis: sequence visualization and annotation.
Kim Rutherford,Julian Parkhill,James Crook,Terry Horsnell,Peter M. Rice,Marie-Adèle Rajandream,Bart Barrell +6 more
TL;DR: Artemis is a DNA sequence visualization and annotation tool that allows the results of any analysis or sets of analyses to be viewed in the context of the sequence and its six-frame translation.
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