Journal ArticleDOI
Development of specific dengue virus 2′-O- and N7-methyltransferase assays for antiviral drug screening
Karine Barral,Corinne Sallamand,Christiane Petzold,Bruno Coutard,Axelle Collet,Yann Thillier,Jan Zimmermann,Jean-Jacques Vasseur,Bruno Canard,Jacques Rohayem,Françoise Debart,Etienne Decroly +11 more
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TLDR
The validated method to generate capped RNA, GpppA(2'OMe)-RNA₇₄, by ligation of two RNA fragments is described, allowing the validation of a rapid and sensitive method easily adaptable for high-throughput inhibitor screening in anti-flaviviral drug development.About:
This article is published in Antiviral Research.The article was published on 2013-09-01. It has received 38 citations till now. The article focuses on the topics: RNA & Dengue virus.read more
Citations
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Journal ArticleDOI
Reversible methylation of m6Am in the 5′ cap controls mRNA stability
Jan Mauer,Xiaobing Luo,Alexandre Blanjoie,Xinfu Jiao,Anya V. Grozhik,Deepak P. Patil,Bastian Linder,Brian F. Pickering,Jean-Jacques Vasseur,Qiuying Chen,Steven S. Gross,Olivier Elemento,Françoise Debart,Megerditch Kiledjian,Samie R. Jaffrey +14 more
TL;DR: Using a transcriptome-wide map of m6Am, it is found that m 6Am-initiated transcripts are markedly more stable than mRNAs that begin with other nucleotides and that m6 am is selectively demethylated by fat mass and obesity-associated protein (FTO).
Journal ArticleDOI
The dengue virus NS5 protein as a target for drug discovery
TL;DR: This review summaries the current knowledge as well as prospective views on both MTase and RdRp activities of dengue virus NS5, which are well characterized, structurally and functionally, and inhibitors of both functions have been identified.
Journal ArticleDOI
Identification of the m6Am Methyltransferase PCIF1 Reveals the Location and Functions of m6Am in the Transcriptome.
Konstantinos Boulias,Konstantinos Boulias,Diana Toczydlowska-Socha,Diana Toczydlowska-Socha,Ben R Hawley,Noa Liberman,Noa Liberman,Ken Takashima,Ken Takashima,Sara Zaccara,Théo Guez,Jean-Jacques Vasseur,Françoise Debart,L. Aravind,Samie R. Jaffrey,Eric L. Greer,Eric L. Greer +16 more
TL;DR: It is found that PCIF1 binds and is dependent on the m7G cap, and the accurate mapping technique will facilitate future studies to characterize m6Am's function.
Journal ArticleDOI
Zika Virus Methyltransferase: Structure and Functions for Drug Design Perspectives.
Bruno Coutard,Karine Barral,Julie Lichière,Barbara Selisko,Baptiste Martin,Wahiba Aouadi,Miguel Ortiz Lombardia,Françoise Debart,Jean-Jacques Vasseur,Jean-Claude Guillemot,Bruno Canard,Etienne Decroly +11 more
TL;DR: It is demonstrated that the ZIKV methyltransferase methylates the mRNA cap and adenosines located in RNA sequences, and conformational specificities highlight the role of a conserved Ser/Arg motif, which participates in RNA and SAM recognition during the reaction turnover.
Journal ArticleDOI
Dengue drug discovery: Progress, challenges and outlook
TL;DR: Characteristics of an ideal DENV antiviral molecule, given the specific immunopathology provoked by this acute viral infection, are described and challenges and opportunities in lead identification activities as well as the path to clinical development of dengue drugs are discussed.
References
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Journal ArticleDOI
Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves.
TL;DR: A general computerized method is developed to describe the dose-response curves in terms of basal and maximal responses, ED50, and curve shape or steepness, which permits rigorous statistical analysis, provides a basis for pooling of information from separate experiments, and allows one to test which characteristics are shared by various curves.
Journal ArticleDOI
Protective efficacy of the recombinant, live-attenuated, CYD tetravalent dengue vaccine in Thai schoolchildren: a randomised, controlled phase 2b trial
Arunee Sabchareon,Derek Wallace,Chukiat Sirivichayakul,Kriengsak Limkittikul,Pornthep Chanthavanich,Saravudh Suvannadabba,Vithaya Jiwariyavej,Wut Dulyachai,Krisana Pengsaa,T. Anh Wartel,Annick Moureau,Melanie Saville,Alain Bouckenooghe,Simonetta Viviani,Nadia Tornieporth,Jean Lang +15 more
TL;DR: These data show for the first time that a safe vaccine against dengue is possible, and Dengue vaccine was well tolerated, with no safety signals after 2 years of follow-up after the first dose.
Journal ArticleDOI
An RNA cap (nucleoside‐2′‐O‐)‐methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization
TL;DR: The results provide a structural basis for the rational design of drugs against the emerging flaviviruses and suggest that the latter is a specific cap‐binding site.
Journal ArticleDOI
Conventional and unconventional mechanisms for capping viral mRNA
TL;DR: The strategies used by viruses of eukaryotic cells to produce functional mRNA 5′-caps and escape innate immunity are reviewed.
Journal ArticleDOI
Structure and function of flavivirus NS5 methyltransferase.
Yangsheng Zhou,Debashish Ray,Yiwei Zhao,Hongping Dong,Suping Ren,Suping Ren,Zhong Li,Yi Guo,Kristen A. Bernard,Kristen A. Bernard,Pei Yong Shi,Pei Yong Shi,Hongmin Li,Hongmin Li +13 more
TL;DR: The results demonstrate that the N-7 methylation activity is essential for the WNV life cycle and, thus, methyltransferase represents a novel target for flavivirus therapy.
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