Structure and functionality in flavivirus NS-proteins: Perspectives for drug design
Michela Bollati,Karin Alvarez,Rene Assenberg,Cécile Baronti,Bruno Canard,Shelley Cook,Bruno Coutard,Etienne Decroly,Xavier de Lamballerie,Ernest A. Gould,Gilda Grard,Jonathan M. Grimes,Rolf Hilgenfeld,Anna M. Jansson,Hélène Malet,Erika J. Mancini,Eloise Mastrangelo,Andrea Mattevi,Mario Milani,Gregory Moureau,Johan Neyts,Raymond J. Owens,Jingshan Ren,Barbara Selisko,Silvia Speroni,Holger Steuber,David I. Stuart,Torsten Unge,Martino Bolognesi +28 more
TLDR
Structural and functional aspects emerging from the characterization of two main components (NS3 and NS5 proteins) of the flavivirus replication complex are reviewed to shed light on the design and development of antiviral drug leads.About:
This article is published in Antiviral Research.The article was published on 2010-08-01 and is currently open access. It has received 296 citations till now. The article focuses on the topics: Flavivirus Infections & European union.read more
Citations
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Ivermectin is a potent inhibitor of flavivirus replication specifically targeting NS3 helicase activity: new prospects for an old drug
Eloise Mastrangelo,Margherita Pezzullo,Tine De Burghgraeve,Suzanne J.F. Kaptein,Boris Pastorino,Kai Dallmeier,Xavier de Lamballerie,Johan Neyts,Alicia M. Hanson,David N. Frick,Martino Bolognesi,Mario Milani +11 more
TL;DR: Ivermectin, a broadly used anti-helminthic drug, proved to be a highly potent inhibitor of YFV replication and inhibited, although less efficiently, the replication of several other flaviviruses, i.e. dengue fever, Japanese encephalitis and tick-borne encephalopathy viruses.
Journal ArticleDOI
In vitro reconstitution of SARS-coronavirus mRNA cap methylation
Mickaël Bouvet,Claire Debarnot,Isabelle Imbert,Barbara Selisko,Eric J. Snijder,Bruno Canard,Etienne Decroly +6 more
TL;DR: It is shown that mRNA cap methylation requires a third viral protein, nsp10, which acts as an essential trigger to complete RNA cap-1 formation, and sensitive in vitro inhibition assays of both activities show that aurintricarboxylic acid, active in SARS-CoV infected cells, targets both MTases with IC50 values in the micromolar range, providing a validated basis for anti-coronavirus drug design.
Journal ArticleDOI
Ten years of dengue drug discovery: Progress and prospects
Siew Pheng Lim,Qing Yin Wang,Christian G. Noble,Yen Liang Chen,Hongping Dong,Bin Zou,Fumiaki Yokokawa,Shahul Nilar,Paul Smith,David Beer,Julien Lescar,Pei Yong Shi +11 more
TL;DR: The knowledge accumulated during the past decade has provided a better rationale for ongoing dengue drug discovery and it is optimistic that this continuous, concerted effort will lead to an effective d Dengue therapy.
Journal ArticleDOI
Crystal Structure and Functional Analysis of the SARS-Coronavirus RNA Cap 2′-O-Methyltransferase nsp10/nsp16 Complex
Etienne Decroly,Claire Debarnot,François Ferron,Mickaël Bouvet,Bruno Coutard,Isabelle Imbert,Laure Gluais,Nicolas Papageorgiou,Andrew J. Sharff,Gérard Bricogne,Miguel Ortiz-Lombardía,Julien Lescar,Julien Lescar,Bruno Canard +13 more
TL;DR: Two other crystal structures are presented, the inhibitor Sinefungin bound in the S-adenosylmethionine binding pocket and the tighter complex nsp10(Y96F)/nsp16, providing the first structural insight into the regulation of RNA capping enzymes in (+)RNA viruses.
Journal ArticleDOI
The flavivirus NS2B-NS3 protease-helicase as a target for antiviral drug development.
TL;DR: The NS2B-NS3 protein is associated with the endoplasmic reticulum membrane via its close interaction with the central hydrophilic region of the integral membrane protein this article.
References
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