Dynamics in the plasma membrane: how to combine fluidity and order
Didier Marguet,Didier Marguet,Pierre-François Lenne,Pierre-François Lenne,Hervé Rigneault,Hervé Rigneault,Hai-Tao He,Hai-Tao He +7 more
TLDR
The basic concepts of Brownian diffusion and lipid domain formation in model membranes are summarized and the development of ideas and tools in this field are tracked, outlining key results obtained on the dynamic processes at work in membrane structure and assembly.Abstract:
Cell membranes are fascinating supramolecular aggregates that not only form a barrier between compartments but also harbor many chemical reactions essential to the existence and functioning of a cell. Here, it is proposed to review the molecular dynamics and mosaic organization of the plasma membrane, which are thought to have important functional implications. We will first summarize the basic concepts of Brownian diffusion and lipid domain formation in model membranes and then track the development of ideas and tools in this field, outlining key results obtained on the dynamic processes at work in membrane structure and assembly. We will focus in particular on findings made using fluorescent labeling and imaging procedures to record these dynamic processes. We will also discuss a few examples showing the impact of lateral diffusion on cell signal transduction, and outline some future methodological challenges which must be met before we can answer some of the questions arising in this field of research.read more
Citations
More filters
Book ChapterDOI
Formalizing spherical membrane structures and membrane proteins populations
TL;DR: A formalization of membrane structure is presented by using a parametric 2-dimensional spherical surface, where membrane proteins reside and can move, according to prescribed operations, thus possibly allowing a global scale analysis of ion flows across a membrane.
Book ChapterDOI
Lateral Diffusion in Heterogeneous Cell Membranes
Didier Marguet,Laurence Salomé +1 more
TL;DR: The physical mechanisms contributing to the creation of inhomogeneity are reviewed and the current methodological approaches allowing us to measure diffusion in living cells are described.
Posted ContentDOI
A simple and powerful analysis of lateral subdiffusion using single particle tracking
TL;DR: The packing coefficient Pc is introduced, which gives an estimate of the degree of free movement that a molecule displays in a period of time independently of its global diffusivity, and can be used to calculate the effective kon and koff of scaffolding interactions such those that immobilize receptors at synapses.
Journal ArticleDOI
Mapping molecular diffusion in the plasma membrane by Multiple-Target Tracing (MTT).
Vincent Rouger,Vincent Rouger,Vincent Rouger,Nicolas Bertaux,Nicolas Bertaux,Nicolas Bertaux,Tomasz Trombik,Tomasz Trombik,Tomasz Trombik,Sébastien Mailfert,Sébastien Mailfert,Sébastien Mailfert,Cyrille Billaudeau,Cyrille Billaudeau,Cyrille Billaudeau,Didier Marguet,Didier Marguet,Didier Marguet,Arnauld Sergé,Arnauld Sergé,Arnauld Sergé +20 more
TL;DR: The goal is to obtain a comprehensive description of molecular processes occurring at cellular membranes in different biological functions by developing analytic tools dedicated to Single-Particle Tracking (SPT) at high density: Multiple-Target Tracing (MTT).
References
More filters
Journal ArticleDOI
The fluid mosaic model of the structure of cell membranes.
S. J. Singer,Garth L. Nicolson +1 more
TL;DR: Results strongly indicate that the bivalent antibodies produce an aggregation of the surface immunoglobulin molecules in the plane of the membrane, which can occur only if the immunoglOBulin molecules are free to diffuse in the membrane.
Journal ArticleDOI
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics
Xavier Michalet,Fabien Pinaud,Laurent A. Bentolila,James M. Tsay,Sören Doose,J. Jack Li,Gobalakrishnan Sundaresan,Anna M. Wu,Sanjiv S. Gambhir,Sanjiv S. Gambhir,Shimon Weiss +10 more
TL;DR: The new generations of qdots have far-reaching potential for the study of intracellular processes at the single-molecule level, high-resolution cellular imaging, long-term in vivo observation of cell trafficking, tumor targeting, and diagnostics.
Journal ArticleDOI
Quantum dot bioconjugates for imaging, labelling and sensing
TL;DR: This review looks at current methods for preparing QD bioconjugates as well as presenting an overview of applications, and concludes that the potential of QDs in biology has just begun to be realized and new avenues will arise as the ability to manipulate these materials improves.
Book
Random walks in biology
TL;DR: This book is a lucid, straightforward introduction to the concepts and techniques of statistical physics that students of biology, biochemistry, and biophysics must know.
Journal ArticleDOI
A guide to choosing fluorescent proteins.
TL;DR: A unified characterization of the best available FPs provides a useful guide in narrowing down the options for biological imaging tools.