Dynamics in the plasma membrane: how to combine fluidity and order
Didier Marguet,Didier Marguet,Pierre-François Lenne,Pierre-François Lenne,Hervé Rigneault,Hervé Rigneault,Hai-Tao He,Hai-Tao He +7 more
TLDR
The basic concepts of Brownian diffusion and lipid domain formation in model membranes are summarized and the development of ideas and tools in this field are tracked, outlining key results obtained on the dynamic processes at work in membrane structure and assembly.Abstract:
Cell membranes are fascinating supramolecular aggregates that not only form a barrier between compartments but also harbor many chemical reactions essential to the existence and functioning of a cell. Here, it is proposed to review the molecular dynamics and mosaic organization of the plasma membrane, which are thought to have important functional implications. We will first summarize the basic concepts of Brownian diffusion and lipid domain formation in model membranes and then track the development of ideas and tools in this field, outlining key results obtained on the dynamic processes at work in membrane structure and assembly. We will focus in particular on findings made using fluorescent labeling and imaging procedures to record these dynamic processes. We will also discuss a few examples showing the impact of lateral diffusion on cell signal transduction, and outline some future methodological challenges which must be met before we can answer some of the questions arising in this field of research.read more
Citations
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Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules
TL;DR: This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies, by comparing diffusion coefficients obtained by FRAP and several other alternative methods.
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Versatile Analysis of Single-Molecule Tracking Data by Comprehensive Testing against Monte Carlo Simulations
TL;DR: An approach for the analysis of single-molecule trajectories which is based on a comprehensive comparison of an experimental data set with multiple Monte Carlo simulations of the diffusion process allows quantitative data analysis, particularly whenever analytical treatment of a model is infeasible.
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Lipid rafts and raft-mediated supramolecular entities in the regulation of CD95 death receptor apoptotic signaling.
TL;DR: The involvement of lipid rafts in the regulation of CD95-mediated apoptosis that may provide a new avenue for cancer therapy is discussed.
Journal ArticleDOI
Nanoscale membrane organization: where biochemistry meets advanced microscopy
Alessandra Cambi,Diane S. Lidke +1 more
TL;DR: The biochemical principles regulating membrane nanodomain formation and protein function, ranging from the lipid nanoenvironment to the cortical cytoskeleton are discussed, and recent advances in fluorescence microscopy are discussed that are making it possible to quantify protein organization and biochemical events at the nanoscale in the living cell membrane.
Journal ArticleDOI
Dynamics of single potassium channel proteins in the plasma membrane of migrating cells.
TL;DR: The results, referring to the role of label size and properties of the extracellular environment, suggest that the pericellular glycocalyx has an important impact on labels used for single molecule tracking
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