Dynamics in the plasma membrane: how to combine fluidity and order
Didier Marguet,Didier Marguet,Pierre-François Lenne,Pierre-François Lenne,Hervé Rigneault,Hervé Rigneault,Hai-Tao He,Hai-Tao He +7 more
TLDR
The basic concepts of Brownian diffusion and lipid domain formation in model membranes are summarized and the development of ideas and tools in this field are tracked, outlining key results obtained on the dynamic processes at work in membrane structure and assembly.Abstract:
Cell membranes are fascinating supramolecular aggregates that not only form a barrier between compartments but also harbor many chemical reactions essential to the existence and functioning of a cell. Here, it is proposed to review the molecular dynamics and mosaic organization of the plasma membrane, which are thought to have important functional implications. We will first summarize the basic concepts of Brownian diffusion and lipid domain formation in model membranes and then track the development of ideas and tools in this field, outlining key results obtained on the dynamic processes at work in membrane structure and assembly. We will focus in particular on findings made using fluorescent labeling and imaging procedures to record these dynamic processes. We will also discuss a few examples showing the impact of lateral diffusion on cell signal transduction, and outline some future methodological challenges which must be met before we can answer some of the questions arising in this field of research.read more
Citations
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Journal ArticleDOI
Lipid rafts: at a crossroad between cell biology and physics.
TL;DR: The concept of lipid rafts as it has emerged from the study of synthetic membranes with the reality of lateral heterogeneity in biological membranes is compared.
Journal ArticleDOI
Coordination of Rho GTPase activities during cell protrusion
Matthias Machacek,Matthias Machacek,Louis Hodgson,Louis Hodgson,Christopher Welch,Hunter L. Elliott,Olivier Pertz,Olivier Pertz,Perihan Nalbant,Amy N. Abell,Gary L. Johnson,Klaus M. Hahn,Gaudenz Danuser +12 more
TL;DR: GTPase coordination in mouse embryonic fibroblasts is examined both through simultaneous visualization of two GTPase biosensors and using a ‘computational multiplexing’ approach capable of defining the relationships between multiple protein activities visualized in separate experiments, finding that RhoA is activated at the cell edge synchronous with edge advancement, whereas Cdc42 and Rac1 are activated 2 μm behind the edge with a delay of 40 s.
Journal ArticleDOI
Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes.
Arnauld Sergé,Nicolas Bertaux,Nicolas Bertaux,Nicolas Bertaux,Hervé Rigneault,Hervé Rigneault,Didier Marguet,Didier Marguet +7 more
TL;DR: An analytical single-particle tracking method and tool, multiple-target tracing (MTT), that takes advantage of the high spatial resolution provided by single-fluorophore sensitivity to generate dynamic maps at high densities of tracked particles, thereby providing global representation of molecular dynamics in cell membranes.
Journal ArticleDOI
Dynamic molecular confinement in the plasma membrane by microdomains and the cytoskeleton meshwork
Pierre-François Lenne,Pierre-François Lenne,L. Wawrezinieck,Fabien Conchonaud,Fabien Conchonaud,Olivier Wurtz,Olivier Wurtz,Annie Boned,Annie Boned,Xiao-Jun Guo,Hervé Rigneault,Hervé Rigneault,Hai-Tao He,Hai-Tao He,Didier Marguet,Didier Marguet +15 more
TL;DR: A free‐like diffusion was observed when both the lipid‐dependent and cytoskeleton‐based organizations were disrupted, which suggests that these are two main compartmentalizing forces at work in the plasma membrane.
Journal ArticleDOI
Organization and Ca2+ Regulation of Adenylyl Cyclases in cAMP Microdomains
TL;DR: The regulation of many of the ACs by the ubiquitous second messenger Ca(2+) provides an overarching mechanism for integrating the activities of these two major signaling systems, and cAMP will exhibit distinct kinetics in discrete cellular domains.
References
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Single-molecule microscopy reveals plasma membrane microdomains created by protein-protein networks that exclude or trap signaling molecules in T cells.
Adam D. Douglass,Ronald D. Vale +1 more
TL;DR: It is shown that the coreceptor CD2, the adaptor protein LAT, and tyrosine kinase Lck cocluster in discrete microdomains in the plasma membrane of signaling T cells, which require protein-protein interactions mediated through phosphorylation of LAT and are not maintained by interactions with actin or lipid rafts.