Mapping brain circuitry with a light microscope.
TLDR
An overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function is presented.Abstract:
The beginning of the 21st century has seen a renaissance in light microscopy and anatomical tract tracing that together are rapidly advancing our understanding of the form and function of neuronal circuits. The introduction of instruments for automated imaging of whole mouse brains, new cell type–specific and trans-synaptic tracers, and computational methods for handling the whole-brain data sets has opened the door to neuroanatomical studies at an unprecedented scale. We present an overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function.read more
Citations
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Journal ArticleDOI
CLARITY for mapping the nervous system
Kwanghun Chung,Karl Deisseroth +1 more
TL;DR: Hydrogel-based structures can be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue, creating a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules.
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Neural Networks of the Mouse Neocortex
Brian Zingg,Houri Hintiryan,Lin Gou,Monica Y. Song,Maxwell Bay,Michael S. Bienkowski,Nicholas N. Foster,Seita Yamashita,Ian Bowman,Arthur W. Toga,Hong-Wei Dong +10 more
TL;DR: Cortico-cortical connectivity map and connectivity matrices revealed that the entire cortex is organized into four somatic sensorimotor, two medial, and two lateral subnetworks that display unique topologies and can interact through select cortical areas.
Journal ArticleDOI
Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging.
Etsuo A. Susaki,Etsuo A. Susaki,Etsuo A. Susaki,Kazuki Tainaka,Kazuki Tainaka,Kazuki Tainaka,Dimitri Perrin,Hiroko Yukinaga,Akihiro Kuno,Hiroki R. Ueda,Hiroki R. Ueda,Hiroki R. Ueda +11 more
TL;DR: A protocol for advanced CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) is described in this paper, which enables simple and efficient organ clearing, rapid imaging by light-sheet microscopy and quantitative imaging analysis of multiple samples.
PatentDOI
Transparent, flexible, low-noise graphene electrodes for simultaneous electrophysiology and neuro-imaging
TL;DR: A transparent, flexible neural electrode technology based on graphene is reported, which enables simultaneous optical imaging and electrophysiological recording and may pave the way for high spatio-temporal resolution electro-optic mapping of the dynamic neuronal activity.
Journal ArticleDOI
A Whole-Brain Atlas of Inputs to Serotonergic Neurons of the Dorsal and Median Raphe Nuclei
Iskra Pollak Dorocic,Daniel Fürth,Yang Xuan,Yvonne Johansson,Laura Pozzi,Gilad Silberberg,Marie Carlén,Konstantinos Meletis +7 more
TL;DR: A comprehensive whole-brain atlas defining the monosynaptic inputs onto forebrain-projecting serotonergic neurons of dorsal versus median raphe based on a genetically restricted transsynaptic retrograde tracing strategy is generated.
References
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TL;DR: In this paper, the authors analyzed synaptic connections with several hundred simultaneous quadruple whole-cell recordings from layer 5 pyramidal neurons in the rat visual cortex and found that the distribution of synaptic connection strength differs significantly from the Poisson distribution and can be fitted by a lognormal distribution.
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Targeted whole−cell recordings in the mammalian brain in vivo
Troy W. Margrie,Axel H. Meyer,Antonio Caputi,Hannah Monyer,Mazahir T. Hasan,Andreas T. Schaefer,Winfried Denk,Michael Brecht +7 more
TL;DR: In this paper, a two-photon targeted patching (TPTP) method was proposed to guide in vivo whole-cell recordings to individual, genetically labeled cortical neurons, which was applied to obtain recordings from genetically manipulated, parvalbumin-EGFP-positive interneurons.
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