Mapping brain circuitry with a light microscope.
TLDR
An overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function is presented.Abstract:
The beginning of the 21st century has seen a renaissance in light microscopy and anatomical tract tracing that together are rapidly advancing our understanding of the form and function of neuronal circuits. The introduction of instruments for automated imaging of whole mouse brains, new cell type–specific and trans-synaptic tracers, and computational methods for handling the whole-brain data sets has opened the door to neuroanatomical studies at an unprecedented scale. We present an overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function.read more
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aMAP is a validated pipeline for registration and segmentation of high-resolution mouse brain data.
Christian J. Niedworok,Christian J. Niedworok,Alexander Brown,Alexander Brown,M. Jorge Cardoso,Pavel Osten,Sebastien Ourselin,Marc Modat,Troy W. Margrie,Troy W. Margrie +9 more
TL;DR: This study empirically evaluates aMAP as a method for registration and subsequent segmentation by validating it against the performance of expert human raters to establish a benchmark standard for mapping the molecular function and cellular connectivity of the rodent brain.
Journal ArticleDOI
Motor cortex — to act or not to act?
TL;DR: Classic and recent findings are highlighted that emphasize that motor cortex neurons have a role in both volitional control and initiation of movements and the ability to suppress undesired movements.
Journal ArticleDOI
Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
Marzena Stefaniuk,Emilio J. Gualda,Monika Pawłowska,Diana Legutko,Paweł Matryba,Paulina Koza,Witold Konopka,Dorota Owczarek,Marcin Wawrzyniak,Pablo Loza-Alvarez,Leszek Kaczmarek +10 more
TL;DR: This work reports on creating novel transgenic rat harboring fluorescent reporter GFP under control of neuronal gene promoter, and presents data on clearing the rat brain, showing that FluoClearBABB was found superior over passive CLARITY and CUBIC methods.
Journal ArticleDOI
Cell-type-specific and projection-specific brain-wide reconstruction of single neurons
Rui Lin,Ruiyu Wang,Jing Yuan,Qiru Feng,Youtong Zhou,Shaoqun Zeng,Miao Ren,Siqi Jiang,Hong Ni,Can Zhou,Hui Gong,Minmin Luo +11 more
TL;DR: A dual-adeno-associated-virus expression system that enables strong and sparse labeling of individual neurons with cell-type and projection specificity and further extended the labeling method for rapid reconstruction in cleared thick brain sections and simultaneous dual-color labeling.
Journal ArticleDOI
Bringing CLARITY to the human brain: visualization of Lewy pathology in three dimensions.
Alan King Lun Liu,Alan King Lun Liu,M. E. D. Hurry,O.T. W. Ng,John DeFelice,Hei Ming Lai,Hei Ming Lai,Ronald K. B. Pearce,G. T‐C. Wong,R. C‐C. Chang,S.M. Gentleman +10 more
TL;DR: This study aimed to compare methodological differences in the application of CLARITY between rodent and large human post mortem brain samples, and investigate if this technique could be used to visualize Lewy pathology in a post mortems Parkinson's brain.
References
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Book
The Mouse Brain in Stereotaxic Coordinates
TL;DR: The 3rd edition of this atlas is now in more practical 14"x11" format for convenient lab use and includes a CD of all plates and diagrams, as well as Adobe Illustrator files of the diagrams, and a variety of additional useful material.
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TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
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A robust and high-throughput Cre reporting and characterization system for the whole mouse brain
Linda Madisen,Theresa A. Zwingman,Susan M. Sunkin,Seung Wook Oh,Hatim A. Zariwala,Hong Gu,Lydia Ng,Richard D. Palmiter,Michael Hawrylycz,Allan R. Jones,Ed S. Lein,Hongkui Zeng +11 more
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Genome-wide atlas of gene expression in the adult mouse brain
Ed S. Lein,Michael Hawrylycz,Nancy Ao,Mikael Ayres,Amy Bensinger,Amy Bernard,Andrew F. Boe,Mark S. Boguski,Mark S. Boguski,Kevin S. Brockway,Emi J. Byrnes,Lin Chen,Li Chen,Tsuey-Ming Chen,Mei Chi Chin,Jimmy Chong,Brian E. Crook,Aneta Czaplinska,Chinh Dang,Suvro Datta,Nick Dee,Aimee L. Desaki,Tsega Desta,Ellen Diep,Tim A. Dolbeare,Matthew J. Donelan,Hong-Wei Dong,Jennifer G. Dougherty,Ben J. Duncan,Amanda Ebbert,Gregor Eichele,Lili K. Estin,Casey Faber,Benjamin A.C. Facer,Rick Fields,Shanna R. Fischer,Tim P. Fliss,Cliff Frensley,Sabrina N. Gates,Katie J. Glattfelder,Kevin R. Halverson,Matthew R. Hart,John G. Hohmann,Maureen P. Howell,Darren P. Jeung,Rebecca A. Johnson,Patrick T. Karr,Reena Kawal,Jolene Kidney,Rachel H. Knapik,Chihchau L. Kuan,James H. Lake,Annabel R. Laramee,Kirk D. Larsen,Christopher Lau,Tracy Lemon,Agnes J. Liang,Ying Liu,Lon T. Luong,Jesse Michaels,Judith J. Morgan,Rebecca J. Morgan,Marty Mortrud,Nerick Mosqueda,Lydia Ng,Randy Ng,Geralyn J. Orta,Caroline C. Overly,Tu H. Pak,Sheana Parry,Sayan Dev Pathak,Owen C. Pearson,Ralph B. Puchalski,Zackery L. Riley,Hannah R. Rockett,Stephen A. Rowland,Joshua J. Royall,Marcos J. Ruiz,Nadia R. Sarno,Katherine Schaffnit,Nadiya V. Shapovalova,Taz Sivisay,Clifford R. Slaughterbeck,Simon Smith,Kimberly A. Smith,Bryan I. Smith,Andy J. Sodt,Nick N. Stewart,Kenda-Ruth Stumpf,Susan M. Sunkin,Madhavi Sutram,Angelene Tam,Carey D. Teemer,Christina Thaller,Carol L. Thompson,Lee R. Varnam,Axel Visel,Axel Visel,Ray M. Whitlock,Paul Wohnoutka,Crissa K. Wolkey,Victoria Y. Wong,Matthew J.A. Wood,Murat B. Yaylaoglu,Rob Young,Brian L. Youngstrom,Xu Feng Yuan,Bin Zhang,Theresa A. Zwingman,Allan R. Jones +109 more
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
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