Mapping brain circuitry with a light microscope.
TLDR
An overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function is presented.Abstract:
The beginning of the 21st century has seen a renaissance in light microscopy and anatomical tract tracing that together are rapidly advancing our understanding of the form and function of neuronal circuits. The introduction of instruments for automated imaging of whole mouse brains, new cell type–specific and trans-synaptic tracers, and computational methods for handling the whole-brain data sets has opened the door to neuroanatomical studies at an unprecedented scale. We present an overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function.read more
Citations
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CLARITY for mapping the nervous system
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Etsuo A. Susaki,Etsuo A. Susaki,Etsuo A. Susaki,Kazuki Tainaka,Kazuki Tainaka,Kazuki Tainaka,Dimitri Perrin,Hiroko Yukinaga,Akihiro Kuno,Hiroki R. Ueda,Hiroki R. Ueda,Hiroki R. Ueda +11 more
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Transparent, flexible, low-noise graphene electrodes for simultaneous electrophysiology and neuro-imaging
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A Whole-Brain Atlas of Inputs to Serotonergic Neurons of the Dorsal and Median Raphe Nuclei
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References
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Whole-brain functional imaging at cellular resolution using light-sheet microscopy
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TL;DR: GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3, which allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general.
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