Mapping brain circuitry with a light microscope.
TLDR
An overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function is presented.Abstract:
The beginning of the 21st century has seen a renaissance in light microscopy and anatomical tract tracing that together are rapidly advancing our understanding of the form and function of neuronal circuits. The introduction of instruments for automated imaging of whole mouse brains, new cell type–specific and trans-synaptic tracers, and computational methods for handling the whole-brain data sets has opened the door to neuroanatomical studies at an unprecedented scale. We present an overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function.read more
Citations
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Journal ArticleDOI
Minimizing Probability Graph Connectivity Cost for Discontinuous Filamentary Structures Tracing in Neuron Image
TL;DR: Wang et al. as mentioned in this paper proposed a graph connectivity theoretical method for precise filamentary structure tracing in neuron image, where they build the initial subgraphs of signals via a region-to-region based tracing method on CNN predicted probability.
The BRAIN Initiative: developing technology to catalyse neuroscience discovery
Lyric A. Jorgenson,William T. Newsome,David J. Anderson,Cornelia I. Bargmann,Emery N. Brown,Emery N. Brown,Karl Deisseroth,John P. Donoghue,Kathy L. Hudson,Geoffrey S.F. Ling,Peter R. MacLeish,Eve Marder,Richard A. Normann,Joshua R. Sanes,Mark J. Schnitzer,Terrence J. Sejnowski,David W. Tank,Roger Y. Tsien,Kamil Ugurbil,John C. Wingfield +19 more
TL;DR: The scientific vision for this initiative set forth by the National Institutes of Health is reviewed and its implications for the future of neuroscience research are discussed, with particular emphasis on its potential impact on the mapping and study of neural circuits.
Journal ArticleDOI
Minimizing Probability Graph Connectivity Cost for Discontinuous Filamentary Structures Tracing in Neuron Image
TL;DR: Experimental results on challenging neuronal images proved that the proposed graph connectivity theoretical method outperformed existing methods and achieved similar results of manual tracing, even in some complex discontinuous issues.
Posted ContentDOI
Identifying weak signals in inhomogeneous neuronal images for large-scale tracing of neurites
Shuai Li,Tingwei Quan,Hang Zhou,Fang-Fang Yin,Anan Li,Fu L,Qingming Luo,Hui Gong,Shaoqun Zeng +8 more
TL;DR: An identification model is constructed that can trace neurites with extremely weak signals against an inhomogeneous background and has the potential to rapidly reconstruct sparsely distributed neurons at the scale of an entire brain.
Journal ArticleDOI
A Versatile Tiling Light Sheet Microscope for Cleared Tissues Imaging
Yanlu Chen,Xiaoliang Li,Dongdong Zhang,Chunhui Wang,Ruili Feng,Xuzhao Li,Yao Wen,Hao Xu,Xinyi Shirley Zhang,Xiao Yang,Yongyi Chen,Yi Feng,Bo O. Zhou,Bi-Chang Chen,Kai Lei,Shang Cai,Jie-Min Jia,Liang Gao +17 more
TL;DR: A tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of centimeter-scale cleared tissues with micron-scale to nanometer-scale spatial resolution and imaging speed is presented.
References
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The Mouse Brain in Stereotaxic Coordinates
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TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
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