Mapping brain circuitry with a light microscope.
TLDR
An overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function is presented.Abstract:
The beginning of the 21st century has seen a renaissance in light microscopy and anatomical tract tracing that together are rapidly advancing our understanding of the form and function of neuronal circuits. The introduction of instruments for automated imaging of whole mouse brains, new cell type–specific and trans-synaptic tracers, and computational methods for handling the whole-brain data sets has opened the door to neuroanatomical studies at an unprecedented scale. We present an overview of the present state and future opportunities in charting long-range and local connectivity in the entire mouse brain and in linking brain circuits to function.read more
Citations
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A new automated 3D detection of synaptic contacts reveals the formation of cortico-striatal synapses upon cocaine treatment in vivo
Nicolas Heck,Nicolas Heck,Nicolas Heck,Marc Dos Santos,Marc Dos Santos,Marc Dos Santos,Brahim Amairi,Brahim Amairi,Brahim Amairi,Marine Salery,Marine Salery,Marine Salery,Antoine Besnard,Etienne Herzog,Etienne Herzog,Thomas Boudier,Peter Vanhoutte,Peter Vanhoutte,Peter Vanhoutte,Jocelyne Caboche,Jocelyne Caboche,Jocelyne Caboche +21 more
TL;DR: It is demonstrated that new cortico-striatal connections are formed in the striatum upon chronic cocaine, and it is shown that the cortical input weight is preserved over other cerebral inputs and that the newly formed spines contact pre-existing axonal boutons.
Journal ArticleDOI
A comparison of manual neuronal reconstruction from biocytin histology or 2-photon imaging: morphometry and computer modeling
TL;DR: Quantitative morphological analysis of the same cells reconstructed using both methods reveals that whilst biocytin reconstructions facilitate tracing of more distal collaterals, both methods are comparable in representing the overall morphology, with FI reconstructions exhibiting smaller depolarizations.
Journal ArticleDOI
On the objectivity, reliability, and validity of deep learning enabled bioimage analyses.
Dennis Segebarth,Matthias Griebel,Nikolai Stein,Cora R. von Collenberg,Corinna Martin,Dominik Fiedler,Lucas B. Comeras,Anupam Sah,Victoria Schoeffler,Teresa M. Lüffe,Alexander Dürr,Rohini Gupta,Manju Sasi,Christina Lillesaar,Maren D. Lange,Ramon O. Tasan,Nicolas Singewald,Hans-Christian Pape,Christoph M. Flath,Robert Blum +19 more
TL;DR: It is shown that ground truth estimation from multiple human annotators helps to establish objectivity in fluorescent feature annotations and ensembles of multiple models trained on the estimated ground truth establish reliability and validity.
Journal ArticleDOI
Dopaminergic dysfunction in neurodevelopmental disorders: recent advances and synergistic technologies to aid basic research
TL;DR: Recent findings exploring dopaminergic function in NDD models are reviewed and the promise of new tools to probe NDD pathophysiology in these circuits are discussed.
Journal ArticleDOI
Toward Whole-Body Connectomics.
Chung-Chuan Lo,Ann-Shyn Chiang +1 more
TL;DR: The current state of connectomics study is discussed, focusing on novel optical approaches and related imaging technologies, and the challenges encountered by scientists who endeavor to map these whole-body connectomes in large animals.
References
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Book
The Mouse Brain in Stereotaxic Coordinates
TL;DR: The 3rd edition of this atlas is now in more practical 14"x11" format for convenient lab use and includes a CD of all plates and diagrams, as well as Adobe Illustrator files of the diagrams, and a variety of additional useful material.
Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
Distributed Hierarchical Processing in the Primate Cerebral Cortex
TL;DR: A summary of the layout of cortical areas associated with vision and with other modalities, a computerized database for storing and representing large amounts of information on connectivity patterns, and the application of these data to the analysis of hierarchical organization of the cerebral cortex are reported on.
Journal ArticleDOI
A robust and high-throughput Cre reporting and characterization system for the whole mouse brain
Linda Madisen,Theresa A. Zwingman,Susan M. Sunkin,Seung Wook Oh,Hatim A. Zariwala,Hong Gu,Lydia Ng,Richard D. Palmiter,Michael Hawrylycz,Allan R. Jones,Ed S. Lein,Hongkui Zeng +11 more
TL;DR: A set of Cre reporter mice with strong, ubiquitous expression of fluorescent proteins of different spectra is generated and enables direct visualization of fine dendritic structures and axonal projections of the labeled neurons, which is useful in mapping neuronal circuitry, imaging and tracking specific cell populations in vivo.
Journal ArticleDOI
Genome-wide atlas of gene expression in the adult mouse brain
Ed S. Lein,Michael Hawrylycz,Nancy Ao,Mikael Ayres,Amy Bensinger,Amy Bernard,Andrew F. Boe,Mark S. Boguski,Mark S. Boguski,Kevin S. Brockway,Emi J. Byrnes,Lin Chen,Li Chen,Tsuey-Ming Chen,Mei Chi Chin,Jimmy Chong,Brian E. Crook,Aneta Czaplinska,Chinh Dang,Suvro Datta,Nick Dee,Aimee L. Desaki,Tsega Desta,Ellen Diep,Tim A. Dolbeare,Matthew J. Donelan,Hong-Wei Dong,Jennifer G. Dougherty,Ben J. Duncan,Amanda Ebbert,Gregor Eichele,Lili K. Estin,Casey Faber,Benjamin A.C. Facer,Rick Fields,Shanna R. Fischer,Tim P. Fliss,Cliff Frensley,Sabrina N. Gates,Katie J. Glattfelder,Kevin R. Halverson,Matthew R. Hart,John G. Hohmann,Maureen P. Howell,Darren P. Jeung,Rebecca A. Johnson,Patrick T. Karr,Reena Kawal,Jolene Kidney,Rachel H. Knapik,Chihchau L. Kuan,James H. Lake,Annabel R. Laramee,Kirk D. Larsen,Christopher Lau,Tracy Lemon,Agnes J. Liang,Ying Liu,Lon T. Luong,Jesse Michaels,Judith J. Morgan,Rebecca J. Morgan,Marty Mortrud,Nerick Mosqueda,Lydia Ng,Randy Ng,Geralyn J. Orta,Caroline C. Overly,Tu H. Pak,Sheana Parry,Sayan Dev Pathak,Owen C. Pearson,Ralph B. Puchalski,Zackery L. Riley,Hannah R. Rockett,Stephen A. Rowland,Joshua J. Royall,Marcos J. Ruiz,Nadia R. Sarno,Katherine Schaffnit,Nadiya V. Shapovalova,Taz Sivisay,Clifford R. Slaughterbeck,Simon Smith,Kimberly A. Smith,Bryan I. Smith,Andy J. Sodt,Nick N. Stewart,Kenda-Ruth Stumpf,Susan M. Sunkin,Madhavi Sutram,Angelene Tam,Carey D. Teemer,Christina Thaller,Carol L. Thompson,Lee R. Varnam,Axel Visel,Axel Visel,Ray M. Whitlock,Paul Wohnoutka,Crissa K. Wolkey,Victoria Y. Wong,Matthew J.A. Wood,Murat B. Yaylaoglu,Rob Young,Brian L. Youngstrom,Xu Feng Yuan,Bin Zhang,Theresa A. Zwingman,Allan R. Jones +109 more
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
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