Retro-orbital injections in mice.
TLDR
The authors report that they have routinely used retro-orbital injection of the venous sinus in the adult mouse to administer volumes up to 150 μl without incident and this technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds.Abstract:
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.read more
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Endothelial hyperactivation of mutant MAP3K3 induces cerebral cavernous malformation enhanced by PIK3CA GOF mutation
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Non-clinical Study of Biodistribution of Human Immature Dental Pulp Stem Cells (Nestacell® Product) Following Intravenous Administration in Mice
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References
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The Mouse in biomedical research
James G. Fox,Stephen W. Barthold,Muriel T. Davisson,Christian E. Newcomer,Fred W. Quimby,Abigail L Smith +5 more
TL;DR: The Mouse in biomedical research , The Mouse inomedical research , مرکز فناوری اطلاعات و £1,000,000; اوشاوρزی; کسورز رسانی ;
Journal ArticleDOI
Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
Hai Song,Kinglun Kingston Mak,Lilia Topol,Kangsun Yun,Jianxin Hu,Lisa Garrett,Yongbin Chen,Ogyi Park,Jia Chang,R. Mark Simpson,Cun-Yu Wang,Bin Gao,Jin Jiang,Yingzi Yang +13 more
TL;DR: It is found that Mst1 and Mst2, the two mouse homologs of the Drosophila Hpo, control the sizes of some, but not all organs, in mice, and MSt1 andMst2 act as tumor suppressors by restricting cell proliferation and survival.
Journal ArticleDOI
Optimal Germinal Center Responses Require a Multistage T Cell:B Cell Adhesion Process Involving Integrins, SLAM-Associated Protein, and CD84
Jennifer L. Cannons,Hai Qi,Kristina T. Lu,Mala Dutta,Mala Dutta,Julio Gomez-Rodriguez,Jun Cheng,Edward K. Wakeland,Ronald N. Germain,Pamela L. Schwartzberg +9 more
TL;DR: The results reveal insight into the dynamic regulation of T cell:B cell interactions and identify SLAM family members as critical components of sustained T cell-B cell adhesion required for productive humoral immunity.
Book
The laboratory mouse
TL;DR: This chapter discusses the development of Phenotyping Terminology and Tests, and some of the techniques used to develop and evaluate these techniques.