Retro-orbital injections in mice.
TLDR
The authors report that they have routinely used retro-orbital injection of the venous sinus in the adult mouse to administer volumes up to 150 μl without incident and this technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds.Abstract:
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.read more
Citations
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Journal ArticleDOI
Neonatal Gene Therapy for Hemophilia B by a Novel Adenovirus Vector Showing Reduced Leaky Expression of Viral Genes
TL;DR: A novel Ad vector is used, Ad-E4-122aT, which exhibits a reduction in the leaky expression of Ad genes in liver, in gene therapy studies for neonatal hemophilia B mice, and exhibited significantly higher transduction efficiencies in neonatal mice.
Journal ArticleDOI
The left lung is preferentially targeted during experimental paracoccidioidomycosis in C57BL/6 mice.
Fabrine Sales Massafera Tristão,Fernanda Agostini Rocha,Fabrício C. Dias,Marcos A. Rossi,João Santana da Silva +4 more
TL;DR: In this article, the authors investigated the differential involvement of the right and left lungs in experimental Paracoccidioidomycosis (PCM) infection by Pb18.
Book ChapterDOI
Visualization of Signaling Molecules During Neutrophil Recruitment in Transgenic Mice Expressing FRET Biosensors.
TL;DR: This chapter describes the protocol used to visualize the protein kinase activities in neutrophils of the inflamed intestine of transgenic mice expressing the FRET biosensors and shows activity changes of protein kinases in the neutrophil recruited to inflamed intestines.
Journal ArticleDOI
Murine isoforms of UDP-GlcNAc 2-epimerase/ManNAc kinase: Secondary structures, expression profiles, and response to ManNAc therapy.
Tal Yardeni,Tal Yardeni,Katherine Jacobs,Terren K. Niethamer,Carla Ciccone,Yair Anikster,Natalya Kurochkina,William A. Gahl,Marjan Huizing +8 more
TL;DR: Gne transcript expression, in particular mGne2, increased significantly, likely resulting in increased Gne enzymatic activities, and the existence and expression of GNE isoforms needs consideration when designing other therapeutic strategies for GNE myopathy.
Journal ArticleDOI
Sodium-Iodate Injection Can Replicate Retinal Degenerative Disease Stages in Pigmented Mice and Rats: Non-Invasive Follow-Up Using OCT and ERG
Céline Koster,Koen T. van den Hurk,Jacoline B. ten Brink,Colby F. Lewallen,Boris V. Stanzel,Kapil Bharti,Arthur A.B. Bergen +6 more
TL;DR: Dose-dependent structural and functional pathological effects on the retinal pigment epithelium (RPE) and retina in the pigmented mouse and rat strains used in this study may serve as useful tools for evaluating novel therapies for RPE-related retinal degenerations, such as AMD.
References
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The Mouse in biomedical research
James G. Fox,Stephen W. Barthold,Muriel T. Davisson,Christian E. Newcomer,Fred W. Quimby,Abigail L Smith +5 more
TL;DR: The Mouse in biomedical research , The Mouse inomedical research , مرکز فناوری اطلاعات و £1,000,000; اوشاوρزی; کسورز رسانی ;
Journal ArticleDOI
Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
Hai Song,Kinglun Kingston Mak,Lilia Topol,Kangsun Yun,Jianxin Hu,Lisa Garrett,Yongbin Chen,Ogyi Park,Jia Chang,R. Mark Simpson,Cun-Yu Wang,Bin Gao,Jin Jiang,Yingzi Yang +13 more
TL;DR: It is found that Mst1 and Mst2, the two mouse homologs of the Drosophila Hpo, control the sizes of some, but not all organs, in mice, and MSt1 andMst2 act as tumor suppressors by restricting cell proliferation and survival.
Journal ArticleDOI
Optimal Germinal Center Responses Require a Multistage T Cell:B Cell Adhesion Process Involving Integrins, SLAM-Associated Protein, and CD84
Jennifer L. Cannons,Hai Qi,Kristina T. Lu,Mala Dutta,Mala Dutta,Julio Gomez-Rodriguez,Jun Cheng,Edward K. Wakeland,Ronald N. Germain,Pamela L. Schwartzberg +9 more
TL;DR: The results reveal insight into the dynamic regulation of T cell:B cell interactions and identify SLAM family members as critical components of sustained T cell-B cell adhesion required for productive humoral immunity.
Book
The laboratory mouse
TL;DR: This chapter discusses the development of Phenotyping Terminology and Tests, and some of the techniques used to develop and evaluate these techniques.