Retro-orbital injections in mice.
TLDR
The authors report that they have routinely used retro-orbital injection of the venous sinus in the adult mouse to administer volumes up to 150 μl without incident and this technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds.Abstract:
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.read more
Citations
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In vivo assessment of bone marrow toxicity by gold nanoparticle-based bioconjugates in Crl:CD1(ICR) mice.
Cristian Berce,Ciprian Lucan,Bobe Petrushev,Sanda Boca,Mirela Miclean,Orsolya Sarpataki,Simion Astilean,Anca Dana Buzoianu,Ciprian Tomuleasa,Anca Bojan +9 more
TL;DR: Au was found to be hepatotoxic, urging the need for further studies in order to achieve better in vivo compliance and exploit the immense potential of GNPs in cancer pharmacology.
Journal ArticleDOI
IR-780-Albumin-Based Nanocarriers Promote Tumor Regression Not Only from Phototherapy but Also by a Nonirradiation Mechanism.
Gustavo Capistrano,Ailton A. Sousa-Junior,Roosevelt Alves da Silva,Francyelli Mello-Andrade,Emílio Ramos Cintra,Sônia F. O. Santos,Allancer D C Nunes,Raisa Melo Lima,Nicholas Zufelato,André Luiz Silva Oliveira,Maristela Pereira,Carlos Henrique de Castro,Eliana Martins Lima,Clever Gomes Cardoso,Elisângela de Paula Silveira-Lacerda,Sebastião Antonio Mendanha,Andris F. Bakuzis +16 more
TL;DR: Two albumin-based nanoparticles containing near-infrared IR-780 iodide multifunctional dyes, one of them possessing a magnetic core are developed and safety studies suggest potential for clinical translation.
Journal ArticleDOI
A signalling pathway for transcriptional regulation of sleep amount in mice
Rui Zhou,Guodong Wang,Qi Li,Fanxi Meng,Can Liu,Rui Gan,Dapeng Ju,Meimei Liao,Junjie Xu,Di Sang,Xue-Ling Gao,Shuang Zhou,Kejia Wu,Quanzhi Sun,Ying Guo,Chongyang Wu,Zhiyu Chen,Lin Chen,Bihan Shi,Haiyan Wang,Xia Wang,Huai Li,Tao Cai,Bin Li,Fengchao Wang,Hiromasa Funato,Masashi Yanagisawa,Eric E. Zhang,Qinghua Liu +28 more
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Optical clearing potential of immersion-based agents applied to thick mouse brain sections.
TL;DR: It is shown that immersion of brain sections in specific OCAs affects both their transparency and volume; the optical clearing effect occurs over the entire visible spectrum and is reversible; and that ScaleSQ had the highest optical clearing potential and increase in imaging depth of the four evaluated OCAs, albeit with the largest change in sample volume and a concomitant decrease in apparent microvascular density of the sample.
Journal ArticleDOI
Best Practices for Preclinical In Vivo Testing of Cancer Nanomedicines.
TL;DR: Best practices for the evaluation of nanomedicines in murine tumor models are discussed, including discussion of the types of data to collect during these studies, and a recommended set of experiments to perform are introduced.
References
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The Mouse in biomedical research
James G. Fox,Stephen W. Barthold,Muriel T. Davisson,Christian E. Newcomer,Fred W. Quimby,Abigail L Smith +5 more
TL;DR: The Mouse in biomedical research , The Mouse inomedical research , مرکز فناوری اطلاعات و £1,000,000; اوشاوρزی; کسورز رسانی ;
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Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
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Optimal Germinal Center Responses Require a Multistage T Cell:B Cell Adhesion Process Involving Integrins, SLAM-Associated Protein, and CD84
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Book
The laboratory mouse
TL;DR: This chapter discusses the development of Phenotyping Terminology and Tests, and some of the techniques used to develop and evaluate these techniques.