Retro-orbital injections in mice.
TLDR
The authors report that they have routinely used retro-orbital injection of the venous sinus in the adult mouse to administer volumes up to 150 μl without incident and this technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds.Abstract:
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.read more
Citations
More filters
Posted ContentDOI
Bright and photostable chemigenetic indicators for extended in vivo voltage imaging
Ahmed S. Abdelfattah,Takashi Kawashima,Amrita Singh,Ondrej Novak,Hui Liu,Yichun Shuai,Yi Chieh Huang,Jonathan B. Grimm,Ronak Patel,Johannes Friedrich,Brett D. Mensh,Liam Paninski,John J. Macklin,Kaspar Podgorski,Bei Jung Lin,Tsai Wen Chen,Glenn C. Turner,Zhe Liu,Minoru Koyama,Karel Svoboda,Misha B. Ahrens,Luke D. Lavis,Eric R. Schreiter +22 more
TL;DR: A novel GEVI is engineered, ‘Voltron’, that utilizes bright and photostable synthetic dyes instead of protein-based fluorophores, extending the combined duration of imaging and number of neurons imaged simultaneously by more than tenfold relative to existing GEVIs.
Journal ArticleDOI
Phosphorylation of the Amyloid-Beta Peptide Inhibits Zinc-Dependent Aggregation, Prevents Na,K-ATPase Inhibition, and Reduces Cerebral Plaque Deposition.
Evgeny P. Barykin,Irina Yu. Petrushanko,Sergey A. Kozin,Georgy B. Telegin,Alexander S. Chernov,Olga D. Lopina,Sergey P. Radko,Vladimir A. Mitkevich,Alexander A. Makarov +8 more
TL;DR: Several AD-associated pathogenic properties of Aβ are neutralized by its phosphorylation, including inhibition of Na+,K+-ATPase activity by Aβ, which is thought to be a major contributor to neuronal dysfunction in AD.
Journal ArticleDOI
Long-circulating Janus nanoparticles made by electrohydrodynamic co-jetting for systemic drug delivery applications.
Sahar Rahmani,Carlos H. Villa,Acacia F. Dishman,Marika Grabowski,Daniel C. Pan,Hakan Durmaz,Asish C. Misra,Laura Colón-Meléndez,Michael J. Solomon,Vladimir R. Muzykantov,Joerg Lahann +10 more
TL;DR: Electrohydrodynamic (EHD) co-jetting is used for the fabrication of uniform anisotropic nanoparticles with individual compartments and initial physicochemical and biological characterization is reported.
Journal ArticleDOI
The CYP2D6 animal model: how to induce autoimmune hepatitis in mice.
TL;DR: A straightforward mouse model that uses the major human autoantigen in type 2 autoimmune hepatitis (AIH-2), namely hCYP2D6, as a trigger and a detailed protocol on how autoimmune liver disease is induced in the CYP2D 6 model is provided and how the different aspects of liver damage can be assessed.
Journal ArticleDOI
Intravenous treatment with human recombinant ApoA-I Milano reduces beta amyloid cerebral deposition in the APP23-transgenic mouse model of Alzheimer's disease
Sofía Fernández de Retana,Alex Montañola,Paula Marazuela,Maialen De La Cuesta,Aina Batlle,Marc Fatar,Saskia Grudzenski,Joan Montaner,Mar Hernández-Guillamon +8 more
TL;DR: Reduced cerebral Aβ levels in mice that received rApoA-I-M are found, which suggest an applicability of this molecule as a therapeutic candidate for protecting the brain in AD.
References
More filters
The Mouse in biomedical research
James G. Fox,Stephen W. Barthold,Muriel T. Davisson,Christian E. Newcomer,Fred W. Quimby,Abigail L Smith +5 more
TL;DR: The Mouse in biomedical research , The Mouse inomedical research , مرکز فناوری اطلاعات و £1,000,000; اوشاوρزی; کسورز رسانی ;
Journal ArticleDOI
Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
Hai Song,Kinglun Kingston Mak,Lilia Topol,Kangsun Yun,Jianxin Hu,Lisa Garrett,Yongbin Chen,Ogyi Park,Jia Chang,R. Mark Simpson,Cun-Yu Wang,Bin Gao,Jin Jiang,Yingzi Yang +13 more
TL;DR: It is found that Mst1 and Mst2, the two mouse homologs of the Drosophila Hpo, control the sizes of some, but not all organs, in mice, and MSt1 andMst2 act as tumor suppressors by restricting cell proliferation and survival.
Journal ArticleDOI
Optimal Germinal Center Responses Require a Multistage T Cell:B Cell Adhesion Process Involving Integrins, SLAM-Associated Protein, and CD84
Jennifer L. Cannons,Hai Qi,Kristina T. Lu,Mala Dutta,Mala Dutta,Julio Gomez-Rodriguez,Jun Cheng,Edward K. Wakeland,Ronald N. Germain,Pamela L. Schwartzberg +9 more
TL;DR: The results reveal insight into the dynamic regulation of T cell:B cell interactions and identify SLAM family members as critical components of sustained T cell-B cell adhesion required for productive humoral immunity.
Book
The laboratory mouse
TL;DR: This chapter discusses the development of Phenotyping Terminology and Tests, and some of the techniques used to develop and evaluate these techniques.