Retro-orbital injections in mice.
TLDR
The authors report that they have routinely used retro-orbital injection of the venous sinus in the adult mouse to administer volumes up to 150 μl without incident and this technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds.Abstract:
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.read more
Citations
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Journal ArticleDOI
Assessment of MRI Contrast Agent Kinetics via Retro-Orbital Injection in Mice: Comparison with Tail Vein Injection.
TL;DR: The retro-orbital route can be used as a simple efficient alternative to tail vein injection for dynamic contrast-enhanced MRI of mice and afforded similar results in terms of the kinetics of the contrast agent.
Journal ArticleDOI
Systemic Administration of Adipose-Derived Stromal Cells Concurrent with Fat Grafting.
TL;DR: Intravenous injection of adipose-derived stromal cells resulted in significantly higher adipogenesis gene expression, retention of graft volume, and vascular density of the graft, which widened the surgical options for fat grafting and enhance the clinical value of cell-assisted lipotransfer.
Journal ArticleDOI
Lateral Flow Assessment and Unanticipated Toxicity of Kratom.
TL;DR: The LD50 values determined for Kratom, its two main psychoactive alkaloids, mitragynine and 7-hydroxymitagynine, are reported as comparable to heroin in mice when administered intravenously, which could be beneficial to law enforcement agencies and health professionals as the opioid epidemic in America continues to evolve.
Journal ArticleDOI
Imaging of cardiac perfusion of free-breathing small animals using dynamic phase-correlated micro-CT.
Stefan Sawall,Jan Kuntz,Michaela Socher,Michael Knaup,Andreas Hess,Sönke Bartling,Marc Kachelrieß +6 more
TL;DR: A low dose scan protocol and an associated reconstruction algorithm that allows for in vivo imaging of cardiac perfusion and associated processes that are retrospectively synchronized to the respiratory and cardiac motion of the animal and reduces metabolic inference to the animal allowing for longitudinal studies.
Journal ArticleDOI
Comparison of Ga-68-Labeled Fusarinine C-Based Multivalent RGD Conjugates and [(68)Ga]NODAGA-RGD-In Vivo Imaging Studies in Human Xenograft Tumors
Chuangyan Zhai,Gerben M. Franssen,Milos Petrik,Peter Laverman,Dominik Summer,Christine Rangger,Roland Haubner,Hubertus Haas,Clemens Decristoforo +8 more
TL;DR: In vitro and in vivo studies showed enhanced integrin αvβ3 binding affinity, receptor-selective tumor uptake, and rapid renal excretion resulting in good imaging properties, with improved properties compared to [68Ga]NODAGA-RGD, making it an alternative for imaging integrinαv β3 expression.
References
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The Mouse in biomedical research
James G. Fox,Stephen W. Barthold,Muriel T. Davisson,Christian E. Newcomer,Fred W. Quimby,Abigail L Smith +5 more
TL;DR: The Mouse in biomedical research , The Mouse inomedical research , مرکز فناوری اطلاعات و £1,000,000; اوشاوρزی; کسورز رسانی ;
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Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
Hai Song,Kinglun Kingston Mak,Lilia Topol,Kangsun Yun,Jianxin Hu,Lisa Garrett,Yongbin Chen,Ogyi Park,Jia Chang,R. Mark Simpson,Cun-Yu Wang,Bin Gao,Jin Jiang,Yingzi Yang +13 more
TL;DR: It is found that Mst1 and Mst2, the two mouse homologs of the Drosophila Hpo, control the sizes of some, but not all organs, in mice, and MSt1 andMst2 act as tumor suppressors by restricting cell proliferation and survival.
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Optimal Germinal Center Responses Require a Multistage T Cell:B Cell Adhesion Process Involving Integrins, SLAM-Associated Protein, and CD84
Jennifer L. Cannons,Hai Qi,Kristina T. Lu,Mala Dutta,Mala Dutta,Julio Gomez-Rodriguez,Jun Cheng,Edward K. Wakeland,Ronald N. Germain,Pamela L. Schwartzberg +9 more
TL;DR: The results reveal insight into the dynamic regulation of T cell:B cell interactions and identify SLAM family members as critical components of sustained T cell-B cell adhesion required for productive humoral immunity.
Book
The laboratory mouse
TL;DR: This chapter discusses the development of Phenotyping Terminology and Tests, and some of the techniques used to develop and evaluate these techniques.