Retro-orbital injections in mice.
TLDR
The authors report that they have routinely used retro-orbital injection of the venous sinus in the adult mouse to administer volumes up to 150 μl without incident and this technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds.Abstract:
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.read more
Citations
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Development of Diverse Size and Shape RNA Nanoparticles and Investigation of their Physicochemical Properties for Optimized Drug Delivery
TL;DR: Construction of diverse size and shape RNA nanoparticles with tunable physicochemical properties, investigation of the effect that size, shape, and nanoparticle properties have on in vivo biodistribution, and development of drug encapsulation and release mechanism utilizing RNA nanotechnology.
Journal ArticleDOI
Orbital Hemorrhagic Necrosis, Globe Rupture, and Death From Intraorbital Injection of 1% Sodium Deoxycholate in a Murine Model.
Nathan W. Blessing,Nathan W. Blessing,Ying Chen,Catherine J. Choi,Jugchawin Kanokkantapong,Wensi Tao,Daniel Pelaez,Bradford W. Lee +7 more
TL;DR: Intraorbital and periocular injection of DCA can cause devastating complications in a murine model, and significant caution is advised for off-label use in the periacular region.
Journal ArticleDOI
A Rodent Model of Mild Neonatal Hypoxic Ischemic Encephalopathy.
TL;DR: In this paper, a 9 min hypoxic cardiac arrest (CA) was induced by stopping mechanical ventilation of intubated, ventilated and curarized rats followed by a cardiopulmonary resuscitation.
Journal ArticleDOI
Optimization of In Vivo Studies by Combining Planar Dynamic and Tomographic Imaging: Workflow Evaluation on a Superparamagnetic Nanoparticles System.
Maritina Rouchota,Alessio Adamiano,Michele Iafisco,Eirini Fragogeorgi,Irineos Pilatis,Gilles Doumont,Sébastien Boutry,Daniele Catalucci,Argyro Zacharioudaki,George C. Kagadis +9 more
TL;DR: In this article, the authors used an iron-based nanoformulation, already established as an MRI/SPECT probe, to investigate its potential for nuclear planar and tomographic imaging of several target tissues following its distribution via different administration routes.
Posted ContentDOI
Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
Xinhong Chen,Damien Wolfe,Dhanesh Sivadasan Bindu,Mengying Zhang,Naz Taskin,David Goertsen,Timothy F. Shay,Erin E. Sullivan,Sheng-Fu Huang,Sripriya Ravindra Kumar,Cynthia Mary Arokiaraj,Viktor M. Plattner,Lillian Campos,John K. Mich,Deja Monet,Victoria Ngo,Xiaozhe Ding,Victoria Omstead,Natalie Weed,Yemeserach Bishaw,Bryan B. Gore,Ed S. Lein,Athena Akrami,Cory T. Miller,Boaz P. Levi,Annika Keller,Jonathan T. Ting,Andrew S. Fox,Cagla Eroglu,Viviana Gradinaru +29 more
TL;DR: In this paper , the authors have evolved adeno-associated virus (AAV9) capsids into vectors that transduce brain endothelial cells specifically and efficiently following systemic administration in wild-type mice with diverse genetic backgrounds and rats.
References
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The Mouse in biomedical research
James G. Fox,Stephen W. Barthold,Muriel T. Davisson,Christian E. Newcomer,Fred W. Quimby,Abigail L Smith +5 more
TL;DR: The Mouse in biomedical research , The Mouse inomedical research , مرکز فناوری اطلاعات و £1,000,000; اوشاوρزی; کسورز رسانی ;
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Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
Hai Song,Kinglun Kingston Mak,Lilia Topol,Kangsun Yun,Jianxin Hu,Lisa Garrett,Yongbin Chen,Ogyi Park,Jia Chang,R. Mark Simpson,Cun-Yu Wang,Bin Gao,Jin Jiang,Yingzi Yang +13 more
TL;DR: It is found that Mst1 and Mst2, the two mouse homologs of the Drosophila Hpo, control the sizes of some, but not all organs, in mice, and MSt1 andMst2 act as tumor suppressors by restricting cell proliferation and survival.
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Optimal Germinal Center Responses Require a Multistage T Cell:B Cell Adhesion Process Involving Integrins, SLAM-Associated Protein, and CD84
Jennifer L. Cannons,Hai Qi,Kristina T. Lu,Mala Dutta,Mala Dutta,Julio Gomez-Rodriguez,Jun Cheng,Edward K. Wakeland,Ronald N. Germain,Pamela L. Schwartzberg +9 more
TL;DR: The results reveal insight into the dynamic regulation of T cell:B cell interactions and identify SLAM family members as critical components of sustained T cell-B cell adhesion required for productive humoral immunity.
Book
The laboratory mouse
TL;DR: This chapter discusses the development of Phenotyping Terminology and Tests, and some of the techniques used to develop and evaluate these techniques.