Retro-orbital injections in mice.
TLDR
The authors report that they have routinely used retro-orbital injection of the venous sinus in the adult mouse to administer volumes up to 150 μl without incident and this technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds.Abstract:
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.read more
Citations
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Hydrophobic Effect from Conjugated Chemicals or Drugs on In Vivo Biodistribution of RNA Nanoparticles
TL;DR: This study examined how the hydrophobicity of chemicals conjugated to RNA nanoparticles affect in vivo biodistribution and provided new insights for handling drug insolubility, therapeutic toxicity, and organ clearance in drug development.
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Sex-Dependent Macromolecule and Nanoparticle Delivery in Experimental Brain Injury
Vimala N. Bharadwaj,Connor Copeland,Ethan Mathew,Jason M. Newbern,Trent Anderson,Jonathan Lifshitz,Jonathan Lifshitz,Vikram D. Kodibagkar,Sarah E. Stabenfeldt +8 more
TL;DR: A sex-dependent temporal profile of blood/brain barrier disruption in a preclinical mouse model of traumatic brain injury (TBI) that contributes to starkly different macromolecule and nanoparticle delivery profiles post-TBI is identified.
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In vivo assessment of specific cytotoxic T lymphocyte killing
T. Clemente,Mariana R. Dominguez,Narciso Junior Vieira,Mauricio M. Rodrigues,Gustavo P. Amarante-Mendes +4 more
TL;DR: A method to evaluate in vivo specific cytotoxic T lymphocyte killing with model system mice immunized with human recombinant replication-deficient adenovirus 5 containing different transgenes as the trigger of a CTL-mediated immune response is described and discussed.
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Comparison of Cesium-137 and X-ray Irradiators by Using Bone Marrow Transplant Reconstitution in C57BL/6J Mice
Brian W. Gibson,Nathan C. Boles,George P. Souroullas,Alan Herron,Joe Kennard Fraley,Rebecca S Schwiebert,John J Sharp,Margaret A. Goodell +7 more
TL;DR: Although both sources ablated endogenous bone marrow sufficiently to enable stem cell engraftment, there are distinct physiologic responses that should be considered when choosing the optimal source for use in a study and that irradiation from the (137)Cs source was associated with lower overall morbidity due to opportunistic infection.
Journal ArticleDOI
Evaluation of Cobalt-Labeled Octreotide Analogs for Molecular Imaging and Auger Electron–Based Radionuclide Therapy
Helge Thisgaard,Helge Thisgaard,Birgitte Brinkmann Olsen,Johan Hygum Dam,Peter Bollen,Jan Mollenhauer,Poul Flemming Høilund-Carlsen +6 more
TL;DR: Evaluated cobalt-labeled octreotide analogs targeting the somatostatin receptor showed considerable therapeutic effects in vitro and, thus, could be an effective agent for eradicating disseminated tumor cells and micrometastases.
References
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The Mouse in biomedical research
James G. Fox,Stephen W. Barthold,Muriel T. Davisson,Christian E. Newcomer,Fred W. Quimby,Abigail L Smith +5 more
TL;DR: The Mouse in biomedical research , The Mouse inomedical research , مرکز فناوری اطلاعات و £1,000,000; اوشاوρزی; کسورز رسانی ;
Journal ArticleDOI
Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
Hai Song,Kinglun Kingston Mak,Lilia Topol,Kangsun Yun,Jianxin Hu,Lisa Garrett,Yongbin Chen,Ogyi Park,Jia Chang,R. Mark Simpson,Cun-Yu Wang,Bin Gao,Jin Jiang,Yingzi Yang +13 more
TL;DR: It is found that Mst1 and Mst2, the two mouse homologs of the Drosophila Hpo, control the sizes of some, but not all organs, in mice, and MSt1 andMst2 act as tumor suppressors by restricting cell proliferation and survival.
Journal ArticleDOI
Optimal Germinal Center Responses Require a Multistage T Cell:B Cell Adhesion Process Involving Integrins, SLAM-Associated Protein, and CD84
Jennifer L. Cannons,Hai Qi,Kristina T. Lu,Mala Dutta,Mala Dutta,Julio Gomez-Rodriguez,Jun Cheng,Edward K. Wakeland,Ronald N. Germain,Pamela L. Schwartzberg +9 more
TL;DR: The results reveal insight into the dynamic regulation of T cell:B cell interactions and identify SLAM family members as critical components of sustained T cell-B cell adhesion required for productive humoral immunity.
Book
The laboratory mouse
TL;DR: This chapter discusses the development of Phenotyping Terminology and Tests, and some of the techniques used to develop and evaluate these techniques.