Retro-orbital injections in mice.
TLDR
The authors report that they have routinely used retro-orbital injection of the venous sinus in the adult mouse to administer volumes up to 150 μl without incident and this technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds.Abstract:
Intravenous vascular access is technically challenging in the adult mouse and even more challenging in neonatal mice. The authors describe the technique of retro-orbital injection of the venous sinus in the adult and neonatal mouse. This technique is a useful alternative to tail vein injection for the administration of non-tumorigenic compounds. The authors report that they have routinely used this technique in the adult mouse to administer volumes up to 150 μl without incident. Administration of retro-orbital injections is more challenging in neonatal mice but can reliably deliver volumes up to 10 μl.read more
Citations
More filters
Posted ContentDOI
Brain-wide measurement of protein turnover with high spatial and temporal resolution
Boaz Mohar,Jonathan B. Grimm,Ronak Patel,Timothy A. Brown,Paul W. Tillberg,Luke D. Lavis,Nelson Spruston,Karel Svoboda +7 more
TL;DR: In this paper , a pulse-chase method was proposed to measure protein turnover with high spatial and temporal resolution throughout the body, which can enable studies of adaptive and maladaptive plasticity in brain-wide neural circuits.
Journal ArticleDOI
Mesothelin/CD3 half-life extended bispecific T-cell engager molecule shows specific tumor uptake and distributes to mesothelin and CD3 expressing tissues.
Frans V. Suurs,Grit Lorenczewski,Julie M. Bailis,Sabine Stienen,Matthias Friedrich,Fei Lee,Bert van der Vegt,Elisabeth G.E. de Vries,Derk-Jan A. de Groot,Marjolijn N. Lub-de Hooge +9 more
TL;DR: In this paper, a fully mouse cross-reactive mesothelin (MSLN)-targeted BiTE molecule that is genetically fused to a Fc-domain for half-life extension was generated and evaluated by PET imaging in BALB/c mice.
Journal ArticleDOI
Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice
Yi Yao,Yun Liu,Akira Takashima +2 more
TL;DR: This protocol provides a fundamental framework for intravital imaging of primed neutrophil behavior and function, which can easily be expanded to examination of other immune cells in mouse inflammation models.
Journal ArticleDOI
Adult re-expression of IRSp53 rescues NMDA receptor function and social behavior in IRSp53-mutant mice
Young Woo Noh,Chaehyun Yook,Jaeseung Kang,Soo Yeon Lee,Yeong Gi Kim,Esther Yang,Hyun Kuk Kim,Eunjoon Kim +7 more
TL;DR: In this article , a brain-blood barrier (BBB)-penetrant adeno-associated virus (AAV) known as PHP.eB was used to drive adult IRSp53 re-expression in mice and normalized social deficits without affecting hyperactivity or anxiety-like behavior.
Journal ArticleDOI
Form-deprivation myopia downregulates calcium levels in retinal horizontal cells in mice.
Qihang Li,He Zhu,Miaomiao Fan,Jing Sun,Peter S. Reinach,Yuhan Wang,Jia Qu,Xiangtian Zhou,Fuxin Zhao +8 more
TL;DR: In this article , a calcium fluorescence indicator system was proposed to monitor horizontal cells' intracellular Ca2+ levels during form-deprivation myopia (FDM) in mice.
References
More filters
The Mouse in biomedical research
James G. Fox,Stephen W. Barthold,Muriel T. Davisson,Christian E. Newcomer,Fred W. Quimby,Abigail L Smith +5 more
TL;DR: The Mouse in biomedical research , The Mouse inomedical research , مرکز فناوری اطلاعات و £1,000,000; اوشاوρزی; کسورز رسانی ;
Journal ArticleDOI
Mammalian Mst1 and Mst2 kinases play essential roles in organ size control and tumor suppression
Hai Song,Kinglun Kingston Mak,Lilia Topol,Kangsun Yun,Jianxin Hu,Lisa Garrett,Yongbin Chen,Ogyi Park,Jia Chang,R. Mark Simpson,Cun-Yu Wang,Bin Gao,Jin Jiang,Yingzi Yang +13 more
TL;DR: It is found that Mst1 and Mst2, the two mouse homologs of the Drosophila Hpo, control the sizes of some, but not all organs, in mice, and MSt1 andMst2 act as tumor suppressors by restricting cell proliferation and survival.
Journal ArticleDOI
Optimal Germinal Center Responses Require a Multistage T Cell:B Cell Adhesion Process Involving Integrins, SLAM-Associated Protein, and CD84
Jennifer L. Cannons,Hai Qi,Kristina T. Lu,Mala Dutta,Mala Dutta,Julio Gomez-Rodriguez,Jun Cheng,Edward K. Wakeland,Ronald N. Germain,Pamela L. Schwartzberg +9 more
TL;DR: The results reveal insight into the dynamic regulation of T cell:B cell interactions and identify SLAM family members as critical components of sustained T cell-B cell adhesion required for productive humoral immunity.
Book
The laboratory mouse
TL;DR: This chapter discusses the development of Phenotyping Terminology and Tests, and some of the techniques used to develop and evaluate these techniques.